Aktivitas Imunomodulator Ekstrak Metanol dan Fraksi buah Talok (Muntingia calabura L.) pada Sel RAW 264.7

Sujono, Tanti Azizah; Kusumowati, Ika Trisharyanti Dian; Munawaroh, Rima

doi:10.20961/jpscr.v6i2.47009

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…Currently, the screening method for metabolites of medicinal plants by binding them to target cells is widely used because it is more effective and efficient. [16][17][18] Macrophages are the main cells in the immune system that regulate the body's defense mechanism against disease. 8 Microorganism infection through the CD14 receptor will activate macrophage cells to release several inflammatory mediators, including pro-inflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Metabolites from Sambiloto (Andrographis paniculata) Ethanol Extract for Pro-Inflammatory Cytokines Inhibitory through In Silico and In Vitro Approaches

Lukito,

Iswantini,

Antariksa

et al. 2024

Pharmacogn J.

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Objective: Andrographis paniculata has long been a traditional medicinal plant in Indonesia. This study is intended to evaluate the anti-pro-inflammatory cytokines of 98% ethanol extract of A. paniculata by in vitro and in silico approaches. Inhibition of pro-inflammatory cytokines is also one of the therapies in treating COVID-19. Methods: The molecular docking approach was utilized as a first screening to evaluate the potential for suppression of macrophage cell activation; an ADMET prediction test was performed to determine the pharmacological, pharmacokinetic, and toxicity as a therapeutic target. TNF-α, IL-1ß, and IL-6 levels were measured using an ELISA method to investigate anti-cytokine pro-inflammatory activity in LPS-induced RAW 264.7 macrophage cells. LC-MS/MS was used to identify additional metabolite compounds. Results: Ethanol extract containing particular metabolites 14-Deoxyandrographoside and 14-Deoxy-17-hydroxyandrographolide inhibited TNF-α and IL-1β by 100% and IL-6 by 85.59%, respectively. While compared to the Dexamethasone molecule as a positive control, preliminary screening and ADMET prediction for the metabolite compound 14-Deoxyandrographoside exhibited relatively high binding stability to the CD14 receptor by -7.5 kcal/mol and was safe against various ADMET indications. Conclusions: This study reveals that the compound 14-Deoxyandrographoside in pure ethanol extract is a potential anticytokine agent candidate for treating pro-inflammatory cytokines, including COVID-19 infection.

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