Akurasi Perhitungan Bakteri pada Daging Sapi Menggunakan Metode Hitung Cawan

Azizah, Azizah; Soesetyaningsih, Endang

doi:10.19184/bst.v8i3.16828

Cited by 8 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the national development policies in the context of realizing animal food sovereignty is directed at increasing beef production [1]. Beef is one of the foodstuffs which has high protein and is the best medium for the growth of microorganisms [2]. This study is in accordance with the opinion of [3] which states that meat is a source of protein which is also a high-value commodity.…”

Section: Introductionsupporting

confidence: 68%

Identification Of Pathogenic Bacteria In Fermented Bekamal Using B14A Isolate

Prastujati

Amaniyah

Priyadi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

The content of protein and nutrients in beef makes microorganisms able to live and breed well, it is easy for pathogenic microorganisms to grow and make beef quickly damaged, so processing technology is needed to extend the shelf life such as fermentation techniques. Bekamal is a fermented beef product from the Banyuwangi district. Bacteria in the bekamal fermentation process are lactic acid bacteria. B14A isolate is assumed as lactic acid bacteria that isolated from bekamal which will be used as a starter for meat fermentation in this study. The purpose of this study is to determine the content of pathogenic microorganisms in fermented bekamal meat using B14A isolate. The samples used in this study were fresh beef and bekamal which was fermented by B14A isolate in 0, 7, 14, and 21 days and the commercial one for 1 month, 2 months, and 3 months. Parameters analyzed include the content of Escherichia coli, Staphylococcus aureus, and Salmonella sp. The method in this study was tested using Qualitative Descriptive Analysis with 8 samples and each consisting of 3 replications. The results showed that all samples of fresh beef were contaminated with three types of pathogens and all samples of bekamal were negative for all three types of pathogens.

show abstract

Section: Introductionsupporting

confidence: 68%

Identification Of Pathogenic Bacteria In Fermented Bekamal Using B14A Isolate

Prastujati

Amaniyah

Priyadi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Koloni per mL = jumlah koloni pada cawan x 1 faktor pengenceran (1) Gambar 4 merupakan hasil perhitungan jumlah koloni bakteri yang terdapat pada air limbah melalui proses seeding dengan pengolahan secara aerob maupun anaerob yang telah dilakukan selama 42 hari. Perhitungan koloni dilakukan menggunakan "Standar Plate Count" dengan cara menghitung dari cawan serta memilih jumlah koloni yang ada di sampel yang layak untuk diaplikasikan [15]. Gambar 4 menunjukkan hasil seeding aerob pada hari ke-7 hingga hari ke-21 aktivitas bakteri/mikroorganisme sangat meningkat.…”

Section: Aklimatisasiunclassified

Proses Seeding Dan Aklimatisasi Aerob – Anaerob Untuk Pengolahan Air Limbah Industri Gondorukem

Amalia,

Prayitno

2024

Distilat: J. Tekn. Sep

View full text Add to dashboard Cite

Air limbah yang dihasilkan oleh industri gondorukem mengandung bahan – bahan organik BOD (Biological Oxygen Demand) dan COD (Chemical Oxygen Demand) yang cukup tinggi sehingga berpotensi menimbulkan pencemaran terhadap lingkungan perairan. Salah satu metode pengolahan air limbah industri gondorukem adalah menggunakan proses biologi (anaerob – aerob) yang melibatkan bakteri anaerob – aerob. Efisiensi pengolahan sangat dipengaruhi oleh jumlah, metabolisme, dan aktivitas dari bakteri tersebut. Selanjutnya metabolisme mikroorganisme sangat ditentukan oleh proses pembenihan (seeding) dan aklimatisasi. Tujuan dari penelitian ini adalah untuk mengetahui laju pertumbuhan mikroorganisme yang terdapat dalam air limbah industri gondorukem. Penelitian dilakukan proses seeding terlebih dahulu yang kemudian dilanjutkan dengan proses aklimatisasi. Sedangkan parameter yang diuji adalah pH, suhu (temperature), dan jumlah mikroorganisme. Hasil penelitian menunjukan bahwa aktivitas mikroorganisme pada aerob telah dapat berkembang biak dengan baik dalam hari ke 14 dengan capaian sebesar 9,1 x 107 dan hari ke 21 sebesar 0,9 x 108. Setelah hari ke 28, aktivitas mikroorganisme menurun mencapai 8,5 x 106. Pada aktivitas mikroorganisme anaerob berkembang baik pada hari ke 28 dengan capaian sebesar 3,1 x 108 lalu menurun di hari ke 35 sebesar 7,7 x 106.

show abstract

“…If the number of colonies is more than 300 CFU/gr it is categorized as too many to count (TBUD). Thus, only plates that grew between 30-300 colonies were counted as the number of bacterial colonies inoculated (Azizah & Soesetyaningsih, 2020).…”

Section: Counting Of Bacteriamentioning

confidence: 99%

Date Palm (Phoenix dactylifera L.) Flour as an Alternative Culture Media for the Growth of Escherichia coli and Bacillus cereus

Rini

Saidi²,

Rohmah³

2023

J.Ilm.Kedokt. Wijaya Kusuma

View full text Add to dashboard Cite

The high cost of bacterial growth culture media can result in obstacles in the process of practicum or research in the field of microbiology. Date palm (Phoenix dactylifera L.) is the oldest fruit plant that grows in the Arabian Peninsula, North Africa and the Middle East. Dates are a source of high energy food with sugar content of 72% - 88%. This research was conducted to test the ability of date palm flour to grow Escherichia coli and Bacillus cereus bacteria as an alternative medium to replace NA media. In this study using total number of bacteria/ TPC (Total Plate Count) method to calculated the number of colonies of bacteria. The results of this study the number of colonies of Escherichia coli bacteria was more than the number of colonies of Bacillus cereus, that is 54 x 105CFU/g on concentration media 8 gr. When compared with NA media, date palm flour media can be used as an inexpensive alternative culture medium for bacteria.

show abstract

Akurasi Perhitungan Bakteri pada Daging Sapi Menggunakan Metode Hitung Cawan

Cited by 8 publications

References 9 publications

Identification Of Pathogenic Bacteria In Fermented Bekamal Using B14A Isolate

Identification Of Pathogenic Bacteria In Fermented Bekamal Using B14A Isolate

Proses Seeding Dan Aklimatisasi Aerob – Anaerob Untuk Pengolahan Air Limbah Industri Gondorukem

Date Palm (Phoenix dactylifera L.) Flour as an Alternative Culture Media for the Growth of Escherichia coli and Bacillus cereus

Contact Info

Product

Resources

About