Alanine Dosimetry Accurately Determines Radiation Dose in Nonhuman Primates

Application of Multivariate Modeling for Radiation Injury Assessment: A Proof of Concept

“…The focus of our study was to develop a multivariate algorithm for calculating the appropriate RC severity for H-ARS with a rhesus monkey TBI model using a 6.5 Gy radiation dose, which based on the literature was predicted to cause RC3 [ 34 ]. In place of the METREPOL methodology, time after irradiation and time-dependent blood variable levels would instead be entered into this multivariate algorithm to estimate an H-ARS RC severity.…”

“…Results from these residual analyses were crucial for determining whether the basic assumptions of linear-least-squares modeling were satisfied by both the CBC and the CBC-SCHEM models. Finally, to determine whether potential problems due to autocorrelation among the independent variables existed, the eigenvalues of the independent variables were computed and evaluated according to criteria developed by Chatterjee and Price [ 12 , 34 ].…”

Real‐time telemetric monitoring in whole‐body 60Co gamma‐photon irradiated rhesus macaques (Macaca mulatta)

“…Once the animal and restraint box were secured, 6.5‐Gy 60 Co γ‐photon radiation was administered at 0.4 Gy/minute, measured at the midpoint of the abdomen. Dosimetry was performed using a water‐filled plastic cylinder matched to the body mass and girth of each animal as previously described [14]. Radiation dose and dose rate were measured using an alanine pellet system that was calibrated to standards from the National Institute of Standards and Technology in Gaithersburg, MD, USA, and from the National Physics Laboratory in the UK.…”