Alcanivorax pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium

Abstract

A taxonomic study was carried out on a novel bacterial strain, designated W11-5 T , which was isolated from a pyrene-degrading consortium enriched from deep-sea sediment of the Pacific Ocean. The isolate was Gram-reaction-negative and oxidase-and catalase-positive. Growth was observed in 0.5-12 % (w/v) NaCl and at 10-42 6C. On the basis of 16S rRNA gene sequence analysis, strain W11-5 T was shown to belong to the genus Alcanivorax with a close relation to A. dieselolei B-5 T (93.9 % 16S rRNA sequence similarity), A. balearicus MACL04 T (93.1 %), A. hongdengensis A-11-3 T (93.1 %), A. borkumensis SK2 T (93.0 %), A. venustensis ISO4 T (93.0 %) and A. jadensis T9 T (92.9 %). Similarities between the gyrB gene sequences of W11-5 T and other species of the genus Alcanivorax were between 76.8 and 80.8 %. The principal fatty acids were C 12 : 0 3-OH (8.0 %), C 16 : 0 (29.1 %) and C 18 : 1 v7c (27.4 %). The G+C content of the chromosomal DNA was 60.8 mol%. Based on its morphology, physiology and fatty acid composition as well as the results of 16S rRNA and gyrB gene sequence analyses, strain W11-5 T (5MCCC 1A00474 T 5CCTCC AB 208236 T 5LMG 25514 T ) represents a novel species of the genus Alcanivorax, for which the name Alcanivorax pacificus sp. nov. is proposed.

Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium

“…In this study, we describe a novel strain, pht-3B T , which was isolated from a pyrene-degrading consortium from an enriched deep-sea sediment sample collected from the western Pacific Ocean (Wang et al, 2008) and shown to be phylogenetically related to members of the genus Nitratireductor. At the time of writing, four novel species from this pyrenedegrading consortium have been described: Roseovarius pacificus , Oceanicola pacificus (Yuan et al, 2009), Bowmanella pacifica (Lai et al, 2009) and Alcanivorax pacificus (Lai et al, 2011a).…”

Alcanivorax nanhaiticus sp. nov., isolated from deep sea sediment

“…(Fernandez-Martínez et al, 2003); 3, A. hongdengensis A-11-3 T (Wu et al, 2009); 4, A. borkumensis SK2 T (Yakimov et al, 1998). Data for catalase, oxidase, API 20NE and API ZYM for the three reference type strains were obtained from our previous study (Lai et al, 2011). The data for strain 19-m-6 T were obtained under the same conditions as used during that study (Lai et al, 2011).…”

“…Data for catalase, oxidase, API 20NE and API ZYM for the three reference type strains were obtained from our previous study (Lai et al, 2011). The data for strain 19-m-6 T were obtained under the same conditions as used during that study (Lai et al, 2011). +, Positive; W, weakly positive; À, negative.…”

“…The genus Alcanivorax, which includes the most important alkane-degrading bacteria in the marine environment, was proposed by Yakimov et al (1998) and, at the time of writing, it comprises ten recognized species, namely Alcanivorax borkumensis (Yakimov et al, 1998), A. jadensis (FernandezMartinez et al, 2003), A. venustensis (Fernandez-Martinez et al, 2003), A. dieselolei (Liu & Shao, 2005a), A. balearicus (Rivas et al, 2007), A. hongdengensis (Wu et al, 2009), A. pacificus (Lai et al, 2011), A. marinus (Lai et al, 2013), A. xenomutans (Rahul et al, 2014) and A. gelatiniphagus (Kwon et al, 2015), all of which were isolated from marine environments. Members of the genus Alcanivorax are Gram-stain-negative, aerobic, motile or non-motile rods that are chemo-organotrophic, tolerate high salt concentrations and obligate hydrocarbonoclastic bacteria (Kwon et al, 2015;Yakimov et al, 1998).…”

“…These results are given in the species description and Table 1. Cells were grown aerobically on modified marine agar (BD) including CH 3 COONa (1.0 g l À1 ) and trisodium citrate (0.5 g l À1 ) with pH 7.5 at 28 C for 72 h, and fatty acids were extracted, saponified and esterified; this was followed by GC analysis of the fatty acid methyl esters according to the instructions of the MIDI system (Sherlock Version 6.0B; TSBA6 Method) (Sasser, 1990). Strain 19-m-6 T was tested under the same conditions as for other type strains of the genus Alcanivorax used during our previous study (Lai et al, 2011). The predominant fatty acids (>10 %) of strain 19-m-6 T were C 16 : 0 (26.0 %), C 18 : 1 !7c (30.8 %) and summed feature 3 (C 16 : 1 !6c and/or C 16 : 1 !7c) (14.6 %) ( Table S1).…”