Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease

Jelski, Wojciech; Wolszczak-Biedrzycka, Blanka; Zasimowicz-Majewska, Elżbieta; Orywal, Karolina; Łapiński, Tadeusz Wojciech; Szmitkowski, Maciej

doi:10.21873/anticanres.12688

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…Jelski et al ( 2008 ) found that class I ADH activity was considerably higher in patients with liver metastases than in subjects with primary liver cancer. In our previous study, the serum activity of total ADH, class I ADH and class II ADH was higher in patients with both alcoholic and non-alcoholic fatty liver diseases than in the control group (Jelski et al 2018a ; Wolszczak-Biedrzycka et al 2022 ).…”

Section: Discussionmentioning

confidence: 89%

“…Numerous studies have demonstrated changes in the activity of alcohol dehydrogenase (ADH), its isoenzymes and aldehyde dehydrogenase (ALDH) in the blood serum of patients with various liver diseases, including hepatitis C, liver tumors, fatty liver disease, and AIH (Chrostek and Szmitkowski 2000 ; Jelski et al 2008 , 2017 , 2018a , 2018b ). These liver diseases showed statistically higher activity of class I ADH and total ADH in the serum of patients than in healthy subjects and the results suggest a potential clinical role for ADH (especially class I ADH) as marker for liver disorders.…”

Section: Introductionmentioning

confidence: 99%

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An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

Wolszczak-Biedrzycka

Bieńkowska

Zasimowicz³

et al. 2022

Arch. Immunol. Ther. Exp.

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Primary biliary cholangitis (PBC; previously known as primary biliary cirrhosis) is a chronic inflammation-induced cholestatic process in the liver. Antimitochondrial antibodies (AMAs) are observed in around 90% of patients, which suggests that PBC is an autoimmune disease. Alcohol dehydrogenase (ADH), ADH isoenzymes and aldehyde dehydrogenase (ALDH) are localized in the liver, and they are useful markers of liver dysfunction. In this study, the activity of total ADH, ADH isoenzymes and ALDH was evaluated in the blood serum of patients with PBC. The experimental group comprised 50 PBC patients, both male and female, aged 28–67. The control group consisted of 50 healthy subjects, both male and female, aged 25–65. The serum activity of class I ADH, class II ADH and ALDH was measured by spectrofluorophotometry, whereas total ADH and class III ADH activity was determined by photometry methods. The activity of class I ADH and total ADH was significantly higher in the experimental group than in the control group (p < 0.001). An increase in class I ADH and total ADH activity indicates that the isoenzyme class I ADH is released by compromised liver cells and can be useful diagnostic markers of PBC.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

Wolszczak-Biedrzycka

Bieńkowska

Zasimowicz³

et al. 2022

Arch. Immunol. Ther. Exp.

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“…Aldehyde dehydrogenase, which catalyze the oxidation of aldehydes to their corresponding carboxylic acids, play a major role in alcohol metabolism. Nonetheless, the activity of alcohol dehydrogenase in nonalcoholic fatty liver disease can also be increased (Jelski et al, 2018b). And previous studies have demonstrated the strong interactions between hepatitis virus and alcohol (McCartney et al, 2008).…”

Section: Discussionmentioning

confidence: 96%

Human Hepatic Cancer Stem Cells (HCSCs) Markers Correlated With Immune Infiltrates Reveal Prognostic Significance of Hepatocellular Carcinoma

Sang

et al. 2020

Front. Genet.

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Background: Several markers have been reported to be specific for hepatic cancer stem cells (HCSCs), which is usually thought to be highly associated with poor clinical outcomes. Tumor-infiltrating immune cells act as an important factor for oncogenesis. Little is known about the correlation of HCSC markers to prognosis and immune infiltrates.Methods: Expression of HCSC markers was analyzed through Oncomine database, Gene Expression Profiling Interactive Analysis (GEPIA) and Integrative Molecular Database of Hepatocellular Carcinoma (HCCDB), respectively. The prognostic effect of HCSC markers was evaluated using Kaplan-Meier plotter in association with different tumor stages, risk factors, and gender. The correlation of HCSC markers to tumor-infiltrating immune cells was tested by Tumor Immune Estimation Resource (TIMER). HCSC markers related gene sets were investigated by GEPIA, with their biological functions being analyzed by Cytoscape software.Results: The expression level of 10 HCSC markers in HCC was higher than that in normal tissues in at least one database. Among them, high expression of CD24, SOX9, and SOX12 was positively correlated with poor prognosis (CD24: OS P = 0.0012, PFS P = 7.9E-05. SOX9: OS P = 0.012. SOX12: OS P = 0.0004, PFS P = 0.0013, respectively). However, the expression of CD13, CD34 and ALDH1A1 was associated with prolonged OS and PFS. SOX12 was significantly upregulated in poor prognosis of HCC patients with different conditions. Besides, total nine HCSC markers were identified to be positively associated with immune infiltration, including SOX12. Furthermore, Toll-like receptor signaling pathway was found to be one major pathway of these HCSC markers related gene networks. Conclusion:Our results suggest that seven upregulated HCSC markers (CD90, EpCAM, CD133, CD24, SOX9, CK19, and SOX12) are related with poor prognosis and immune infiltration in HCC. In addition, we find that high SOX12 expression remarkably affect prognosis in male HCC patients but not in female. HCC patients under viral infection or

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“…In our previous investigations we have found that the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were signi cantly higher in the serum of patients with different liver diseases (e.g. hepatitis C, autoimmune hepatitis, fatty liver disease) than in healthy subjects [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

The Comparison of Total Bile Acid Concentration and Alcohol Dehydrogenase Activity as Markers of Intrahepatic Cholestasis of Pregnancy

Piechota

Jelski

Orywal

et al. 2021

Preprint

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Background: Intrahepatic cholestasis of pregnancy (ICP) is the liver disorder in the second or early third trimester of pregnancy. It is characterized by pruritus with increased serum total bile acids concentration (TBA) and increased liver enzymes. It is important to recognize the disease in its early stage. We aimed to investigate the serum alcohol dehydrogenase (ADH) activity and compare it with the concentration of total bile acid (TBA) in women with ICP..Methods: Serum samples were taken from 80 pregnancies with ICP in the second or third trimester of pregnancy and from 80 healthy pregnant women. For measurement of ADH we used the spectrofluorometric and photometric methods. Results: The results shows a statistically significant increase in the activity of ADH I and ADH total (about 60% and 41%, respectively). Activity of ADH I well correlated with aminotransferases (alanine ALT and aspartate AST) and TBA concentration. The total ADH activity was also positively correlated with ALT, AST and TBA. Conclusion: We can state that the activity of class I ADH isoenzyme in the sera of patients with ICP is increased and seems to be a good indicator of liver cell destruction during ICP and is comparable with the value of other markers.

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Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease

Abstract: The activity of isozymes of class I and II alcohol dehydrogenase in patients with NAFLD is enhanced and appears to be due to the release of these isoenzymes from damaged hepatocytes.

Cited by 7 publications

References 18 publications

An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

Human Hepatic Cancer Stem Cells (HCSCs) Markers Correlated With Immune Infiltrates Reveal Prognostic Significance of Hepatocellular Carcinoma

The Comparison of Total Bile Acid Concentration and Alcohol Dehydrogenase Activity as Markers of Intrahepatic Cholestasis of Pregnancy

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