Alcoholic  extract  of  Tarantula  cubensis  (Theranekron®)  induce  autophagy  on  gastric cancer cells

Yenigün, Vildan Betül; Azzawri, Ali Ahmed; Acar, Mustafa Said; Kaplan, Muhammed Burak; Uçar, Vasfiye Betül; Taştekin, Didem; Acar, Hasan

doi:10.30714/j-ebr.2021267971

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…However, cell death due to TCAE is not specific to cancer cells, as it was also observed in healthy cells. It has been suggested that cell death may be related to the Ethanol used in the extraction process [38]. It was found in the study that TCAE inhibited tumor development and proliferation and stimulated non-mutagenic tumor suppressor genes [39].…”

Section: Crc + Tcae Crc + Capecitabine Crc + Tcae + Capecitabinementioning

confidence: 99%

Effect of Tarantula cubensis alcohol extract and Capecitabin combine in Colorectal Cancer rats

2023

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Colon cancer (CRC) is one of the most common types of cancer in the world. In this study, the effects of Tarantula cubensis alcoholic extract (TCAE) and the Capecitabine in CRC were investigated. Wistar albino rats were divided into eight groups with 12 animals in each group: untreated healthy and CRC groups, healthy and CRC groups treated with TCAE or Capecitabine, and healthy and CRC groups treated with both TCAE and Capecitabine. Azoxymethane was used in all CRC groups. TCAE and Capecitabine were administered to the relevant groups starting in the 15th week. All rats were euthanized after 18 weeks, and tissue samples were collected. The mRNA levels of Bcl–2, Bax, and Cas–3 in the harvested tissues were determined using real–time PCR and histopathologically abnormal crypt foci (ACF) scores were determined. It was found that TCAE modulated the decreased Bax/Bcl–2 expression rate in the CC group, but had the opposite effect in healthy animals, which was significantly reduced compared to the healthy groups (P<0.05). In addition, this rate was significantly lower in Capecitabine administered groups compared to other groups, and a paradoxical effect was observed (P<0.05). No significant change was observed in Cas–3 expression levels in all groups (P>0.05). Importantly, single and combined use of TCAE and Capecitabine in rats with CRC significantly reduced ACF scores (P<0.05). It can be stated that TCAE can specifically modulate the decreased Bax/Bcl–2 ratio in animals with cancer, and the therapeutic efficacy of Capecitabine is achieved at a dose of 40 mg·kg-1.

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Section: Crc + Tcae Crc + Capecitabine Crc + Tcae + Capecitabinementioning

confidence: 99%

Effect of Tarantula cubensis alcohol extract and Capecitabin combine in Colorectal Cancer rats

2023

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Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells

İlhan

2021

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Tarantula cubensis is known as Cuban tarantula having a venom that contains a diverse mixture of potent compounds with various biological activities. These peptides have been shown to have antitumor activities, therefore features of spider-venom peptides prompted scientists to test them as a potential anticancer drug. The purpose of the study was to investigate the potential cytotoxic effect of Tarantula cubensis venom (Logoplex®) on human cancer cells including prostate (PC-3), lung (H69), breast (MDA-MB-231), and ovarian (OVCAR-3). Moreover, non-tumorigenic MCF-10A cells were used to evaluate the possible cancer cell-specific effect of the extract. The increasing concentrations of Logoplex® were applied for 24, 48 and 72 h. MTT assay was used to assess cell viability. Concentration-response curves and the IC50 values were determined via Graphpad Prism software. Logoplex® caused a time-and concentration-dependent cytotoxic effect in MDA-MB-231, PC-3, OVCAR-3 and MCF-10A cells and the highest cytotoxicity was achieved at 72h. However, in H69 cells, there was a concentration-dependent cytotoxic effect and the highest cytotoxicity was achieved at 24h. IC50 values of Logoplex® in MDA-MB-231, OVCAR-3, PC-3, H69 and MCF-10A cells were determined as 159.3±2.1, 48.9±1.8, 40.2±1.2, 498.3±1.2 and 217.8±2.0 µg/mL, respectively. Logoplex® showed a lower cytotoxic effect against normal cells than the cancer cells suggesting a cancer cell-specific effect. According to the preliminary results of this study, although Logoplex® is a veterinary drug, its cytotoxic effect on human cancer cells suggests that it should be re-evaluated as a potential cytotoxic agent. Analyses to identify functional compounds of Tarantula cubensis venom, and future studies addressing its mechanism of action on cancer cells are recommended.

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Protective Effect of Nerium oleander Distillate and Tarantula cubensis Alcoholic Extract on Cancer Biomarkers in Colon and Liver Tissues of Rats with Experimental Colon Cancer

Dik

Coşkun

2022

ACAMC

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Background: Colon cancers are among the three major cancer types that result in death. The research for effective treatment continues. Objective: The aim of this study is to determine the effects of Tarantula cubensis alcoholic extract (TCAE) and Nerium oleander (NO) distillate on the levels of midkine, TGF-β, VEGF, AFP, COX-2, IGF and caspase 3 in liver and colon tissues of experimentally induced colon cancer in rats. Method: The liver and colon tissues of the rats were divided into Control, Colon Cancer (AZM), AZM+TCAE and AZM+NO groups and they were homogenized. The levels of midkine, TGF-β, VEGF, AFP, COX-2, IGF and caspase 3 in the colon and liver tissues were measured by ELISA kits. Results: All parameters levels of colon and liver tissues in the AZM group were generally higher (p<0.05) than the Control group. TCAE and NO prevented (p<0.05) the increases in midkine, TGF-β, VEGF, AFP, COX-2, IGF and caspase-3 levels in the colon. NO prevented increase of all parameters except for IGF level, while TCAE prevented (p<0.05) the increase of all values apart from COX-2 and IGF levels in the liver. Conclusion: NO and TCAE may prevented at the specified marker levels of colon in the AZM induced colon cancer. The increases the level of parameters in the liver are not as severe as in the colon, due to the 18-week study period may not be sufficient for liver metastasis formationIn the future molecular studies should be done to determine the mechanisms and pathways of them more clearly.

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Alcoholic extract of Tarantula cubensis (Theranekron®) induce autophagy on gastric cancer cells

Cited by 3 publications

References 18 publications

Effect of Tarantula cubensis alcohol extract and Capecitabin combine in Colorectal Cancer rats

Effect of Tarantula cubensis alcohol extract and Capecitabin combine in Colorectal Cancer rats

Can a Veterinary Drug be Repurposed for Human Cancers?: Cytotoxic Effect of Tarantula cubensis Venom on Human Cancer Cells

Protective Effect of Nerium oleander Distillate and Tarantula cubensis Alcoholic Extract on Cancer Biomarkers in Colon and Liver Tissues of Rats with Experimental Colon Cancer

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