Alginate as an immobilization material for MAb production via encapsulated hybridoma cells

Selimoglu, S. Mert; Elibol, Murat

doi:10.3109/07388550903451652

Cited by 36 publications

(26 citation statements)

References 71 publications

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“…Alginate is the most commonly used crosslinking material to prepare the immobilized microbial cells, which are achieved by crosslinking the carboxyl group of α-l-guluronic acid with a cationic cross-linker such as calcium chloride, forming an egg-box model (Fig. 8g) (Katam and Bhattacharyya 2019;Selimoglu and Elibol 2010;Wang et al 2019a). The beads that are generated from the alginate have multi-functions as a carrier material of microbial cells, including (1) formation of a biofilm composed of the native microbes and the immobilized microbes on the surface of the beads (Fig.…”

Section: Immobilized Microorganisms On Biocharmentioning

confidence: 99%

“…Mechanisms of immobilized microorganisms on biochar and carrier material alginate. a The sorption of microbial cells on biochar surface, b formation of biofilm composing of native microbes and the immobilized microbes on biochar surface, c inhibition of the penetration of the native microbes from outsides into the beads and prevention of the liberation of the immobilized microorganisms into the outside environment, d Permission of an uninterrupted reaction that is carried out by the microbes, e The entrapment of microbial cells in crosslinking material alginate, f The combination of the sorption of microbial cells on biochar and the entrapment in crosslinking material, g crosslinking between the carboxyl group of α-l-guluronic acid with a calcium cation, forming an egg-box structure(Selimoglu and Elibol 2010), h biochar as electron shuttle may facilitate the degradation of contaminants by the laccase enzyme attached on biochar…”

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confidence: 99%

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Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Wang

et al. 2020

Biochar

144

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The development of biochar has triggered a hot-spot in various research fields including agriculture, energy, environment, and materials. Biochar-based materials provide a novel approach against environmental challenging issues. Considering the rapid development of biochar materials, this review serves as a valuable platform to summarize the recent progress on the theoretical investigation and engineering applications of biochar materials in environmental remediation. For a better understanding of the structure-application relationships, the structural properties of biochar from macroscopic and microscopic aspects are summarized. The multilevel structures including elements, phases, surface chemistry, and molecular are highlighted to elucidate the multi-functional properties of biochars. Sorption, catalysis, redox reaction, and biological activity of biochar are briefly illustrated, which influence the transport, transformation, and removal of organic and inorganic pollutants in the environments. According to the multi-level structures and structure-application relationships of biochar, specific biocharbased materials and devices have been designed for practical environmental application. The important progress on the functionalization and device of biochar-based materials, including magnetic biochars, 2D and 3D biochar-based macrostructures, immobilized microorganism on biochar, and biochar-amended biofilters are highlighted. The environmental friendliness and sustainability of biochar-based materials, considering the whole cycle from synthesis to application, are evaluated.

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Section: Immobilized Microorganisms On Biocharmentioning

confidence: 99%

mentioning

confidence: 99%

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Wang

et al. 2020

Biochar

144

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“…In bioMEMS devices, chitosan films have been used for drug delivery, redox and small molecule detection, biocatalysis, and cell studies 20,23,24,25 . Similarly, alginate is widely used as a cell-entrapment matrix and has been explored for reversible fluidic containment of cell populations and in-film immunoanalysis 26,27,28 . Composite films for tissue engineering applications have been fabricated using alginate electrodeposition, with components such as with hydroxyapatite for orthopedic implants 29 .…”

Section: Discussionmentioning

confidence: 99%

Bridging the Bio-Electronic Interface with Biofabrication

Gordonov

Liba

Terrell

et al. 2012

JoVE

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Advancements in lab-on-a-chip technology promise to revolutionize both research and medicine through lower costs, better sensitivity, portability, and higher throughput. The incorporation of biological components onto biological microelectromechanical systems (bioMEMS) has shown great potential for achieving these goals. Microfabricated electronic chips allow for micrometer-scale features as well as an electrical connection for sensing and actuation. Functional biological components give the system the capacity for specific detection of analytes, enzymatic functions, and whole-cell capabilities. Standard microfabrication processes and bio-analytical techniques have been successfully utilized for decades in the computer and biological industries, respectively. Their combination and interfacing in a lab-on-a-chip environment, however, brings forth new challenges. There is a call for techniques that can build an interface between the electrode and biological component that is mild and is easy to fabricate and pattern.Biofabrication, described here, is one such approach that has shown great promise for its easy-to-assemble incorporation of biological components with versatility in the on-chip functions that are enabled. Biofabrication uses biological materials and biological mechanisms (selfassembly, enzymatic assembly) for bottom-up hierarchical assembly. While our labs have demonstrated these concepts in many formats 1,2,3 , here we demonstrate the assembly process based on electrodeposition followed by multiple applications of signal-based interactions. The assembly process consists of the electrodeposition of biocompatible stimuli-responsive polymer films on electrodes and their subsequent functionalization with biological components such as DNA, enzymes, or live cells 4,5 . Electrodeposition takes advantage of the pH gradient created at the surface of a biased electrode from the electrolysis of water 6,7 ,. Chitosan and alginate are stimuli-responsive biological polymers that can be triggered to self-assemble into hydrogel films in response to imposed electrical signals 8 . The thickness of these hydrogels is determined by the extent to which the pH gradient extends from the electrode. This can be modified using varying current densities and deposition times 6,7 . This protocol will describe how chitosan films are deposited and functionalized by covalently attaching biological components to the abundant primary amine groups present on the film through either enzymatic or electrochemical methods 9,10 . Alginate films and their entrapment of live cells will also be addressed 11 . Finally, the utility of biofabrication is demonstrated through examples of signal-based interaction, including chemical-to-electrical, cell-to-cell, and also enzyme-to-cell signal transmission.Both the electrodeposition and functionalization can be performed under near-physiological conditions without the need for reagents and thus spare labile biological components from harsh conditions. Additionally, both chitosan and alginate ...

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“…IgG'ler komplement sistemin klasik yolağını aktive etmekte ve toksinlere bağlanarak nötralize olmalarını sağlamaktadır. Antikora bağlı hücresel sitotoksisitede ve sitozolde bulunan işaretlenmiş viriyonların imha edilmesini sağlayan hücre içi antikor yardımlı proteoliz mekanizmasında da önemli rol oynarlar 1,2 . Tüm bunların yanı sıra, IgG yaklaşık 22 günlük yarı ömrü ile kanda kalış süresi en uzun olan antikor tipidir.…”

Section: Introductionunclassified

Monoklonal Antikor Teknolojisinin Dünü, Bugünü Ve Geleceği

Selimoglu¹,

Kasap²,

Akpınar³

et al. 2016

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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When Paul Erlich postulated the idea of "magic bullet" in the early part of 20th century, he only had the findings of a research, regarding the presence of humoral immunity, performed by Emil von Behring and Kitasato Shibasaburo.. There was no concrete data about the presence of antibodies in those days. According to the idea, "if a compound could be made that selectively targeted a disease-causing organism, then a toxin for that organism could be delivered along with the agent of selectivity". If the point in the technology of monoclonal antibody generation that we have reached from '80s to present is considered, it can be claimed that we have started to go beyond the technology realizing the fighting strategy principally proposed by Paul Erlich at those times. The period starting with the production of fully murine antibodies in the first years exhibited a fast-growing trend with the help of recombinant

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Alginate as an immobilization material for MAb production via encapsulated hybridoma cells

Cited by 36 publications

References 71 publications

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Bridging the Bio-Electronic Interface with Biofabrication

Monoklonal Antikor Teknolojisinin Dünü, Bugünü Ve Geleceği

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