Alginate-Based Biomaterials for Regenerative  Medicine Applications

Sun, Jinchen; Tan, Huaping

doi:10.3390/ma6041285

Cited by 1,128 publications

(770 citation statements)

References 148 publications

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“…The ability of cells to bind alginate chains and form network structure has been widely explored in cell and tissue engineering (28,29). Alginate is not cell interactive and hence, when the cells are added to non-modified alginate solutions in the absence of chemical cross-linker, the cell-cell interactions dominate, leading to cell aggregation and creation of non-homogeneous gel network.…”

Section: Preparation and Optimisation Of Probiotic Encapsulated In MImentioning

confidence: 99%

Lactobacillus casei loaded Soy Protein-Alginate Microparticles prepared by Spray-Drying

Hadzieva

Mladenovska

Crcarevska

et al. 2017

Food Technol. Biotechnol.

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SummaryThis article presents a novel formulation for preparation of Lactobacillus casei 01 encapsulated in soy protein isolate and alginate microparticles using spray drying method. A response surface methodology was used to optimise the formulation and the central composite face-centered design was applied to study the effects of critical material attributes and process parameters on viability of the probiotic after microencapsulation and in simulated gastrointestinal conditions. Spherical microparticles were produced in high yield (64 %), narrow size distribution (d 50 =9.7 μm, span=0.47) and favourable mucoadhesive properties, with viability of the probiotic of 11.67, 10.05, 9.47 and 9.20 log CFU/g after microencapsulation, 3 h in simulated gastric and intestinal conditions and four-month cold storage, respectively. Fourier-transform infrared spectroscopy confirmed the probiotic stability after microencapsulation, while differential scanning calorimetry and thermogravimetry pointed to high thermal stability of the soy protein isolate-alginate microparticles with encapsulated probiotic. These favourable properties of the probiotic microparticles make them suitable for incorporation into functional food or pharmaceutical products.

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Section: Preparation and Optimisation Of Probiotic Encapsulated In MImentioning

confidence: 99%

Lactobacillus casei loaded Soy Protein-Alginate Microparticles prepared by Spray-Drying

Hadzieva

Mladenovska

Crcarevska

et al. 2017

Food Technol. Biotechnol.

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“…It forms a gel easily by temperature control but has a melting temperature of 30-35°C [81] , which is below the standard physiological temperature of 37°C. Similarly, alginate produces gels easily through cation crosslinking, but unless it is modified with motifs that can guide cells to adhere, proliferate and differentiate, it is relatively inert [82] .…”

Section: Natural Materialsmentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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“…Alginate has found wide applications as a scaffold for drug delivery. It is biocompatible and shows non antigenicity [4]. Alginate was also proposed as a candidate material for transplantable artificial ovary matrices in 2012 [3], [5].…”

Section: Introductionmentioning

confidence: 99%

A Mechanical Model for an Artificial Oocyte

Hajiyavand¹,

Saadat²,

Stamboulis³

2017

IJMO

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Abstract-Cell injection to introduce a foreign material in biological cells is currently an important area of research. Intervention with biological cells such as an oocyte poses a major limitation towards understanding their biomechanical behaviour, especially during injection, which could result in significant and undesirable deformation of the outer membrane. In this paper, a cross-linked alginate hydrogel is proposed as a material to develop an artificial oocyte to simulate the mechanical properties of a natural human oocyte and use it for mechanical analysis and modelling. A mechanical model and finite element analysis are first presented. The relationship between the injection force and the mechanical properties of the gel are then studied and presented. To validate the mechanical model, experiments were performed using different diameters of an oocyte made using the proposed hydrogel, showing that the modelling results satisfy the experimental demands and that this model can be used to estimate the mechanical properties of other gels too.Index Terms-Artificial oocyte, alginate, hydrogel, finite element model, deformation. I. INTRODUCTIONMany researchers have focused on micro-injection technology which has been proved to be an effective technique introducing foreign materials into cells. This technology can be applied for gene injection, In-vitro fertilisation (IVF), intra-cytoplasmic sperm injection (ICSI), drug development and other biomedical areas notably in cancer treatments [1]. To the best knowledge of the authors, this is the first report of proposing a material for fabricating an artificial oocyte.Accessing human biological oocyte samples for experimental work is very limited, and therefore having suitable artificial samples that could mimic the natural tissues would help to develop appropriate experimental methodology.Synthetic and natural polymers could be considered for the development of artificial tissues. In 2017 alginate hydrogels were proposed for transplantation of ovarian follicles considering different grafting times [2]. Alginate is one of the proposed materials which has the minimum grafting time that is reported to be one week [3]. Alginate

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Alginate-Based Biomaterials for Regenerative Medicine Applications

Cited by 1,128 publications

References 148 publications

Lactobacillus casei loaded Soy Protein-Alginate Microparticles prepared by Spray-Drying

Lactobacillus casei loaded Soy Protein-Alginate Microparticles prepared by Spray-Drying

3D bioprinting for tissue engineering: Stem cells in hydrogels

A Mechanical Model for an Artificial Oocyte

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