Alginate encapsulation of genetically engineered mammalian cells: Comparison of production devices, methods and microcapsule characteristics

Koch, Sandra; Schwinger, Christian; Kreßler, Jörg; Heinzen, Ch.; Rainov, Nikolai G.

doi:10.3109/02652040309178071

Cited by 73 publications

(43 citation statements)

References 27 publications

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“…Larger alginate microspheres ͑500-2000 m͒ can easily be generated via standard droplet generation techniques such as air-jet break-up. 20,21 However, in our experience it is significantly more difficult to produce spheres with a diameter of Ͻ400 m using these conventional methods. By virtue of having decreased diffusion paths, beads with smaller diameter are arguably more effective in averting cell hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

et al. 2007

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Cellular transplantation is a promising technology with great clinical potential in regenerative medicine and disease management. However, effective control over patient immunological response is essential. The encapsulation of cells within hydrogel microspheres is an increasingly prevalent method for the protection of cellular grafts from immune rejection. Microfluidic "chip" reactors present elegant solutions to several capsule generation issues, including the requirement for intercapsule uniformity, high reproducibility, and sterile, good manufacturing practice compliance. This study presents a novel method for the on-chip production of stable, highly monodisperse alginate microspheres and demonstrates its utility in the encapsulation of an immortalized human-derived cell line. Four populations of immortalized human embryonic kidney cells ͑HEK293͒ were encapsulated on chip within monodisperse alginate capsules. Cell viability measurements were recorded for each of the four encapsulated populations for 90 days.

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Section: Discussionmentioning

confidence: 99%

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

et al. 2007

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“…Several solutions have already been suggested to solve this problem, such as the use of cell selection, [9][10][11] dynamic cell culture (flow perfusion system), [12][13][14][15][16] and encapsulation of cells in microspheres. [17][18][19][20] The final cell population has to be incorporated in a scaffold material that possesses intrinsic osteophilic properties. For bone purposes, the ideal scaffold material is considered to be bioactive, slowly biodegradable, moldable, and easy to apply.…”

Section: Introduction Cmentioning

confidence: 99%

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

Link¹,

Dolder²,

Wolke³

et al. 2006

Tissue Engineering

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In this study, the cytocompatibility and early osteogenic characteristics of rat bone marrow cells (RBMCs) on injectable calcium phosphate (CaP) cement (Calcibon) were investigated. In addition to unmodified CaP cement discs, 2 other treatments were given to the discs: preincubation in MilliQ and sintering at different temperatures. After primary culture, RBMCs were dropwise seeded on the discs and cultured for 12 days. The samples were evaluated in terms of cell viability, morphology (live and dead assays and scanning electron microscopy (SEM)), cell proliferation (deoxyribonucleic acid (DNA) analyses), early cell differentiation (alkaline phosphatase (ALP) activity), and physicochemical analyses (xray diffraction (XRD)). The live and dead, DNA, and SEM results showed that Calcibon discs without any additional treatment were not supporting osteoblast-like cells in vitro. There were fewer cells, and cell layers were detached from the disc surface. Therefore, different preincubation periods and sintering temperatures were evaluated to improve the cytocompatibility of the CaP cement. Preincubating discs in MilliQ for periods of 1, 4, 8, and 12 weeks resulted in the hydrolysis of a-tri calcium phosphate (TCP) into an apatite-like structure with some b-TCP, as shown with XRD, but the material was not cytocompatible. Sintering the discs between 8008C and 11008C resulted in conversion of a-TCP to b-TCP with some hydroxyapatite and an increase in crystallinity. Eventually, the discs sintered at 11008C achieved better cell attachment, more-abundant cell proliferation, and earlier differentiation than other sintered (6008C, 8008C, and 10008C), preincubated, and unmodified specimens. On basis of our results, we conclude that in vivo results with CaP-based cements do not guarantee in vitro applicability. Furthermore, unmodified Calcibon is not cytocompatible in vitro, although preincubation of the material results in a more-favorable cell response, sintering of the material at 11008C results in the best osteogenic properties. In contrast to in vivo studies, the Calcibon CaP cement is not suitable as a scaffold for cellbased tissue-engineering strategies.

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“…3) (7, 8). The vibration nozzle technique is considered to be the most industrially up-scalable technique for microcapsule production, especially when the viscosity of the encapsulation solution is low (51). Although laminar jet breakup and air-jet encapsulation technologies have high throughput, it is difficult to obtain evenly sized capsules (52, 53).…”

Section: Bioencapsulation Materials and Methodsmentioning

confidence: 99%

Bioencapsulation Technologies in Tissue Engineering

Majewski

Zhang

et al. 2016

Journal of Applied Biomaterials & Functional Materials

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Bioencapsulation technologies have played an important role in the developing successes of tissue engineering. Besides offering immunoisolation, they also show promise for cell/tissue banking and the directed differentiation of stem cells, by providing a unique microenvironment. This review describes bioencapsulation technologies and summarizes their recent progress in research into tissue engineering. The review concludes with a brief outlook regarding future research directions in this field.

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Alginate encapsulation of genetically engineered mammalian cells: Comparison of production devices, methods and microcapsule characteristics

Cited by 73 publications

References 27 publications

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

The Cytocompatibility and Early Osteogenic Characteristics of an Injectable Calcium Phosphate Cement

Bioencapsulation Technologies in Tissue Engineering

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