Alginate Nanofibrous Mats with Adjustable Degradation Rate for Regenerative Medicine

Hajiali, Hadi; Heredia‐Guerrero, José A.; Liakos, Ioannis; Athanassiou, Athanassia; Mele, Elisa

doi:10.1021/bm501834m

Cited by 49 publications

(31 citation statements)

References 48 publications

(63 reference statements)

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“…The scaffold were soaked in phosphate‐buffered saline (PBS at pH = 7.4) and incubated at 37 °C. At specified times specimens were taken out from the incubator, washed with dionized water and vacuum‐dried at 40 °C for 48 h . Mass loss of the scaffold was calculated over a 2‐month time period using Equation .

Mass loss (() %) = ((), ((), Wo - Wt) / Wo) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Mohammadi

Ramakrishna

Laurent

et al. 2018

J Biomedical Materials Res

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Providing affinity sites on alginate (ALG) matrix enables specific binding of growth factors to the polymer backbone and allows their release in a controlled fashion. In this study, we used a blend of alginate sulfate (ALG‐S) and polyvinyl alcohol (PVA) to fabricate electrospun scaffolds capable of delivering a heparin‐like growth factor, transforming growth factor‐beta1 (TGF‐β1). The alginate was sulfated with different degrees of sulfation (DS, from 0.8, 3.4 to 12.4%) by a simple process. The success of sulfation was determined by Fourier‐transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), elemental analysis, ultraviolet (UV) spectroscopy and staining with dimethylmethylene blue. The physical‐mechanical properties of nanofibrous mats were characterized by scanning electron microscopy (SEM), FTIR, energy‐dispersive X‐ray spectroscopy (EDX), tensile strength and mass loss analysis. Additionally, the release kinetics of transforming growth factor‐β1 (TGF‐β1) from PVA/ALG‐S and PVA/ALG scaffolds were compared. The results showed that the binding and entrapment of TGF‐β1 to the nanofibrous scaffolds are improved by the addition of sulfate group to alginate. In conclusion, our results support that nanofibrous scaffold based on PVA/ALG‐S can deliver growth factors in tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 403–413, 2019.

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Mass loss (() %) = ((), ((), Wo - Wt) / Wo) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Mohammadi

Ramakrishna

Laurent

et al. 2018

J Biomedical Materials Res

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“…In particular, nanofibers produced by electrostatic spinning have high potentiality in the wound healing field because their porosity promotes nutrient transport and gas permeation, their morphological organization mimics the native tissue, and their mechanical properties can be engineered. 5,[9][10][11] The intrinsic high surface area of nanofibers is also attractive for the delivery of drugs and active agents. 2,12 Alginate structures cross-linked with Calcium ions (Ca 2+ ) are mainly used for the treatment of highly exuding wounds and burns.…”

Section: Introductionmentioning

confidence: 99%

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

et al. 2016

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This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication.Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available.You can find more information about Accepted Manuscripts in the Information for Authors. One of the current challenges in wound care is the development of multifunctional dressings that can both protect the wound from external agents and promote the regeneration of the new tissue. Here, we show the combined use of two naturally derived compounds, sodium alginate and lavender essential oil, for the production of bioactive nanofibrous dressings by electrospinning, and their efficacy for the treatment of skin burns induced by midrange ultraviolet radiation (UVB). We demonstrate that the engineered dressings reduce the risk of microbial infection of the burn, since they stop the growth of Staphylococcus aureus. Furthermore, they are able to control and reduce the inflammatory response that is induced in human foreskin fibroblasts by lipopolysaccharides, and in rodents by UVB exposure. In particular, we report a remarkable reduction of pro-inflammatory cytokines when fibroblasts or animals are treated with the alginate-based nanofibers. The down-regulation of cytokines production and the absence of erythema on the skin of the treated animals confirm that the here described dressings are promising as advanced biomedical devices for burn management.

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“…chitosan, sodium alginate, hyaluronic acid, etc.) usually need to be electrospun in blend form by mixing them with synthetic polymers such as PEO, PVA or PVP, which improves their electrospinning processability [4][5][6][7][8]. High specific area, tunable pore size, controlled mechanical properties, and their ability to interact with cells in a manner which mimics the natural ECMs mainly cause that fibrous materials are finding an increasing range of applications, including biomedical areas e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Alginate was found to possess many significant and desirable properties as material for wound dressing such as decent water absorptivity, conformability, optimal water vapor transmission rate, and mild antiseptic properties coupled with non-toxicity, nonimmunogenicity, and biocompability [4,8,18]. In particular, when alginate is combined with well-known biocides (e.g.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of electrospun alginate nanofibers loaded with ciprofloxacin hydrochloride

Kyzioł

Michna

Moreno

et al. 2017

European Polymer Journal

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Alginate nanofibers were prepared in the presence of poly(ethylene oxide) (PEO), a surfactant Pluronic F-127, and a model drug (ciprofloxacin hydrochloride, CpHCl), all mixed prior to electrospinning. It was demonstrated that addition of a carrier polymer (PEO) and a small amount of surfactant are necessary to obtain uniform alginate fibers with cylindrical shape and regular morphology. Importantly, PEO was completely removed from the resulting nanofibers during crosslinking and stabilization post-treatment. The stable alginate fibers loaded with CpHCl were examined by scanning electron microscopy and the average diameter of the fibers ranged from 109 nm (unloaded fibers) to 161 nm (loaded fibers). The release of a studied antibiotic from the nanofibers, characterized by a final loading efficiency of 51%, was tested in physiological conditions. It was revealed that ca. 24% of CpHCl is released during first 20h with combined transport mechanism, however with the predominant contribution of Fickian diffusion.

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Alginate Nanofibrous Mats with Adjustable Degradation Rate for Regenerative Medicine

Cited by 49 publications

References 48 publications

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Fabrication of Nanofibrous PVA/Alginate‐Sulfate Substrates for Growth Factor Delivery

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

Preparation and characterization of electrospun alginate nanofibers loaded with ciprofloxacin hydrochloride

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