Alginate stabilized gold nanoparticle as multidrug carrier: Evaluation of cellular interactions and hemolytic potential

Dey, Soma; Sherly, M. Caroline Diana; Rekha, M.R.; Sreenivasan, K.

doi:10.1016/j.carbpol.2015.09.016

Cited by 49 publications

(25 citation statements)

References 32 publications

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“…The effect of CUR was attributed only to its capacity of blocking P-gp because this drug demonstrated cytotoxicity against Calu-3 at concentrations higher than 50 µmol.L −1 as represented in Table 2. Dey et al [20] studied alginate-stabilized gold nanoparticles containing CUR and MTX and investigated their effect on C6 glioma and MCF-7 cancer cells. Cell viability was reduced by the use of CUR:MTX ratios of 42:41 and 21:20.5 µmol•L −1 .…”

Section: Cell Viability By Mtt and Srb Testsmentioning

confidence: 99%

“…Some controlled release strategies have been reported in the literature concerning the co-delivery of CUR and MTX. Dey et al [20] developed gold nanoparticles containing CUR and MTX and evaluated their cytotoxic effect on C6 glioma cells and MCF-7 breast cancer cells. Curcio et al (2018) [21] obtained pH-responsive polymersomes by self-assembling of a carboxyl-terminated PEG amphiphile achieved via esterification of PEG diacid with PEG40stearate.…”

Section: Introductionmentioning

confidence: 99%

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Co-Loaded Curcumin and Methotrexate Nanocapsules Enhance Cytotoxicity against Non-Small-Cell Lung Cancer Cells

et al. 2020

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Background: As part of the efforts to find natural alternatives for cancer treatment and to overcome the barriers of cellular resistance to chemotherapeutic agents, polymeric nanocapsules containing curcumin and/or methotrexate were prepared by an interfacial deposition of preformed polymer method. Methods: Physicochemical properties, drug release experiments and in vitro cytotoxicity of these nanocapsules were performed against the Calu-3 lung cancer cell line. Results: The colloidal suspensions of nanocapsules showed suitable size (287 to 325 nm), negative charge (−33 to −41 mV) and high encapsulation efficiency (82.4 to 99.4%). Spherical particles at nanoscale dimensions were observed by scanning electron microscopy. X-ray diffraction analysis indicated that nanocapsules exhibited a non-crystalline pattern with a remarkable decrease of crystalline peaks of the raw materials. Fourier-transform infrared spectra demonstrated no chemical bond between the drug(s) and polymers. Drug release experiments evidenced a controlled release pattern with no burst effect for nanocapsules containing curcumin and/or methotrexate. The nanoformulation containing curcumin and methotrexate (NCUR/MTX-2) statistically decreased the cell viability of Calu-3. The fluorescence and morphological analyses presented a predominance of early apoptosis and late apoptosis as the main death mechanisms for Calu-3. Conclusions: Curcumin and methotrexate co-loaded nanocapsules can be further used as a novel therapeutic strategy for treating non-small-cell lung cancer.

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Section: Cell Viability By Mtt and Srb Testsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co-Loaded Curcumin and Methotrexate Nanocapsules Enhance Cytotoxicity against Non-Small-Cell Lung Cancer Cells

et al. 2020

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“…These NPs use active targeting pathway to penetrate into MCF-7 cells due to the presence of the "anti-folate" drug, MTX. The NPs' ZP was -25.8 mV and their average size was 187 nm (197). Folate receptor and reduced folate carriers are two systems which are involved in MTX internalization (198).…”

Section: Targeting Of Cnps To Cancer Cells Overexpressing Folate Recementioning

confidence: 99%

Drug Targeting Strategies Based on Charge Dependent Uptake of Nanoparticles into Cancer Cells

et al. 2019

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The aim of this review was to describe the preferred charged nano-particles (CNPs) for targeted delivery in tumor cells. Zeta Potential (ZP), which represents the surface charge of NPs was highlighted in cell entrance and interactions. In this regard, various types of endocytosis pathways which are involved in NPs’ uptake were first introduced. Then, significance of positively charged NPs (PCNPs) in proton sponge effect corresponding to lysosomal escape was discussed. Cells prefer to endocyte the NPs with positive charge in passive targeting and gene delivery, while in active targeting; the charge of receptors’ ligand binding site determines the NPs cellular uptake. Moreover, pH-sensitive NPs represent charge reversible behavior depending on pH changes which leads to longer blood circulation residence and higher uptake at acidic microenvironment of the cancer media. Role of the CNPs in overcoming multidrug resistance (MDR) and bypassing p-glycoprotein was further investigated.

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“…These NPs exerted a cytotoxic effect on C6 glioma and MCF-7 cancer-cell lines and were found to be hemocompatible. Their active targeting against MCF-7 cancer cells was due to the presence of the Methotrexate [183]. In the case of polysaccharides, chitosan is one of the most used of these biomedical applications due to its biocompatibility and biodegradability.…”

Section: Drug Nanocarriersmentioning

confidence: 99%

Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

et al. 2019

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The fundamental aspects of the manufacturing of gold nanoparticles (AuNPs) are discussed in this review. In particular, attention is devoted to the development of a simple and versatile method for the preparation of these nanoparticles. Eco-friendly synthetic routes, such as wet chemistry and biosynthesis with the aid of polymers, are of particular interest. Polymers can act as reducing and/or capping agents, or as soft templates leading to hybrid nanomaterials. This methodology allows control of the synthesis and stability of nanomaterials with novel properties. Thus, this review focus on a fundamental study of AuNPs properties and different techniques to characterize them, e.g., Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), UV-Visible spectroscopy, Dynamic Light Scattering (DLS), X-Ray Diffraction (XRD), X-Ray Photoelectron Spectroscopy, Small-angle X-Ray Scattering (SAXS), and rheology. Recently, AuNPs obtained by “green” synthesis have been applied in catalysis, in medicine, and as antibacterials, sensors, among others.

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Alginate stabilized gold nanoparticle as multidrug carrier: Evaluation of cellular interactions and hemolytic potential

Cited by 49 publications

References 32 publications

Co-Loaded Curcumin and Methotrexate Nanocapsules Enhance Cytotoxicity against Non-Small-Cell Lung Cancer Cells

Co-Loaded Curcumin and Methotrexate Nanocapsules Enhance Cytotoxicity against Non-Small-Cell Lung Cancer Cells

Drug Targeting Strategies Based on Charge Dependent Uptake of Nanoparticles into Cancer Cells

Nanoengineering of Gold Nanoparticles: Green Synthesis, Characterization, and Applications

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