Aliens at the border and cadavers in the field: a molecular technique for species identification

Armstrong, Karen F.; Cameron, Clare; Frampton, E. R.; Suckling, D. M.

doi:10.30843/nzpp.1997.50.11323

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…Detection mechanisms vary across pathways, but once intercepted an organism's identity must be established before its route of entry may be suppressed (Armstrong et al. ). Real‐time PCR assays are DNA‐dependent; therefore, the lack of symptoms of the disease or immature life stages of the pest do not necessarily limit accurate diagnosis.…”

Section: Discussionmentioning

confidence: 99%

A real‐time PCR toolbox for accurate identification of invasive fruit fly species

Dhami

Gunawardana

Voice

et al. 2015

J Applied Entomology

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Significant plant pests such as fruit flies that travel with fresh produce between countries as eggs or larvae pose a great economic threat to the agriculture and fruit industry worldwide. Time‐limited and expensive quarantine decisions require accurate identification of such pests. Immature stages are often impossible to identify, making them a serious concern for biosecurity agencies. Use of COI barcoding PCR, often the only molecular identification resource, is time‐consuming. We assess the suitability of the COI barcoding region for real‐time PCR assays to identify four pest fruit fly species (Family: Tephritidae), in a diagnostic framework. These species, namely Mediterranean fruit fly (Ceratitis capitata), Queensland fruit fly (Bactrocera tryoni), African invader fly (Bactrocera invadens) and Island fly (Dirioxa pornia) each provide a different set of genetic species delimitation problems. We discuss the benefits and limitations of using a single‐gene TaqMan™ real‐time approach for such species. Our results indicate that COI‐based TaqMan™ real‐time PCR assays, in particular for genetically distinct species, provide an accurate, sensitive and rapid diagnostic tool.

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Section: Discussionmentioning

confidence: 99%

A real‐time PCR toolbox for accurate identification of invasive fruit fly species

Dhami

Gunawardana

Voice

et al. 2015

J Applied Entomology

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“…Since 1994 a molecular diagnostic technique based on PCR-RFLP of the ITS1, 5.8S plus ITS2 rDNA regions (Armstrong et al 1997b), has been used routinely to rapidly identify fruit fly intercepted at the New Zealand border to the species level. Also, in a similar manner to the tussock moth method, a speciesspecific PCR amplifying a 200 bp nested region has been used for the identification of degraded DNA associated with eggs found in cooked breadfruit (unpublished).…”

Section: Testing Cox1 Dna Barcodes For Exotic Insect Identificationmentioning

confidence: 99%

DNA barcodes for biosecurity: invasive species identification

Armstrong

Ball

2005

Phil. Trans. R. Soc. B

489

421

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Biosecurity encompasses protecting against any risk through 'biological harm', not least being the economic impact from the spread of pest insects. Molecular diagnostic tools provide valuable support for the rapid and accurate identification of morphologically indistinct alien species. However, these tools currently lack standardization. They are not conducive to adaptation by multiple sectors or countries, or to coping with changing pest priorities. The data presented here identifies DNA barcodes as a very promising opportunity to address this. DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach. Species identifications were compared with the historical dataset obtained by PCR-RFLP of nuclear rDNA. There was 90 and 96% agreement between the methods for these species, respectively. Improvements included previous tussock moth 'unknowns' being placed to family, genera or species and further resolution within fruit fly species complexes. The analyses highlight several advantages of DNA barcodes, especially their adaptability and predictive value. This approach is a realistic platform on which to build a much more flexible system, with the potential to be adopted globally for the rapid and accurate identification of invasive alien species.

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“…Fruit flies (Diptera: Tephritidae) represent a major biosecurity threat to New Zealand's horticulture sector, worth in excess of $ NZ 2 billion in 2005 1. Over 250 species of fruit flies present varying degrees of risk to New Zealand,2 and the absence of fruit fly species represents an export market advantage that is highly valued. The Ministry of Agriculture and Forestry, Biosecurity New Zealand (MAFBNZ) operates a nationwide surveillance trapping programme, with about 7385 Lynfield traps3, 4 deployed at 3518 sites that are checked fortnightly 5.…”

Section: Introductionmentioning

confidence: 99%