Aligned natural–synthetic polyblend nanofibers for peripheral nerve regeneration

Wang, Chunyang; Zhang, Kuihua; Fan, Cunyi; Mo, Xiumei; Ruan, Hongjiang; Li, Fengfeng

doi:10.1016/j.actbio.2010.09.011

Cited by 165 publications

(109 citation statements)

References 37 publications

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“…[ 57] variations in conduit design: multi-channelled collagen conduit (1-, 2-, 4-, 7-channel conduits) rat sciatic 10 mm 16 weeks 4-channel conduits significantly decreased axonal dispersion versus control single channel conduits.…”

Section: Luminal Wall Guidance Features: Enhancing Porosity and Incrementioning

confidence: 99%

“…Another similar luminal wall guidance strategy involves the use of electrospun fibrous conduit (table 3) [25,27,40,[55][56][57][58]61]. The use of these aligned electrospun tubes has a number of advantages over continuous tube strategies: (i) the materials are highly flexible and porous, and these are well adapted for use within biological systems; (ii) nano-and micro-scale fibres have a high surface area-to-volume ratio increasing the area available for protein absorption, SC migration and regeneration of axons; (iii) fibres that can be preferentially aligned resulting in increased SC alignment, proliferation and growth, and the promotion of guided axonal growth [13,40,62].…”

Section: Luminal Wall Guidance Features: Enhancing Porosity and Incrementioning

confidence: 99%

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A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

Daly

Yao

Zeugolis

et al. 2011

J. R. Soc. Interface.

492

508

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Microsurgical techniques for the treatment of large peripheral nerve injuries (such as the gold standard autograft) and its main clinically approved alternative-hollow nerve guidance conduits (NGCs)-have a number of limitations that need to be addressed. NGCs, in particular, are limited to treating a relatively short nerve gap (4 cm in length) and are often associated with poor functional recovery. Recent advances in biomaterials and tissue engineering approaches are seeking to overcome the limitations associated with these treatment methods. This review critically discusses the advances in biomaterial-based NGCs, their limitations and where future improvements may be required. Recent developments include the incorporation of topographical guidance features and/or intraluminal structures, which attempt to guide Schwann cell (SC) migration and axonal regrowth towards their distal targets. The use of such strategies requires consideration of the size and distribution of these topographical features, as well as a suitable surface for cell-material interactions. Likewise, cellular and molecular-based therapies are being considered for the creation of a more conductive nerve microenvironment. For example, hurdles associated with the short half-lives and low stability of molecular therapies are being surmounted through the use of controlled delivery systems. Similarly, cells (SCs, stem cells and genetically modified cells) are being delivered with biomaterial matrices in attempts to control their dispersion and to facilitate their incorporation within the host regeneration process. Despite recent advances in peripheral nerve repair, there are a number of key factors that need to be considered in order for these new technologies to reach the clinic.

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Section: Luminal Wall Guidance Features: Enhancing Porosity and Incrementioning

confidence: 99%

Section: Luminal Wall Guidance Features: Enhancing Porosity and Incrementioning

confidence: 99%

A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

Daly

Yao

Zeugolis

et al. 2011

J. R. Soc. Interface.

492

508

View full text Add to dashboard Cite

show abstract

“…All procedures used were described in our previous study. 36 To investigate the extent of nerve remyelination, we assessed the ultrastructure of myelin sheaths under a TEM and MBP expression by immunohistochemistry staining. A 2-mm segment was harvested from the injury site and fixed in cold buffered 3% glutaraldehyde solution then processed for TEM (JEOL JEM 2010 system).…”

Section: Gene Transfection Efficiencymentioning

confidence: 99%

Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush

Song¹,

Li²,

Li³

et al. 2017

IJN

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MicroRNA (miRNA) has great potential to treat a wide range of illnesses by regulating the expression of eukaryotic genes. Biomaterials with high transfection efficiency and low toxicity are needed to deliver miRNA to target cells. In this study, a biodegradable and biocompatible cationic polymer (PDAPEI) was synthetized from low molecular weight polyethyleneimine (PEI1.8kDa) cross-linked with 2,6-pyridinedicarboxaldehyde. PDAPEI showed a lower cytotoxicity and higher transfection efficiency than PEI25kDa in transfecting miR-221/222 into rat Schwann cells (SCs). The upregulation of miR-221/222 in SCs promoted the expression of nerve growth factor and myelin basic protein in vitro. The mouse sciatic nerve crush injury model was used to evaluate the effectiveness of PDAPEI/miR-221/222 complexes for nerve regeneration in vivo. The results of electrophysiological tests, functional assessments, and histological and immunohistochemistry analyses demonstrated that PDAPEI/miR-221/222 complexes significantly promoted nerve regeneration after sciatic nerve crush, specifically enhancing remyelination. All these results show that the use of PDAPEI to deliver miR-221/222 may provide a safe therapeutic means of treating nerve crush injury and may help to overcome the barrier of biomaterial toxicity and low efficiency often encountered during medical intervention.

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“…27,28 Polymer nanofibers seem to be good materials for endothelial cells. [29][30][31] Some studies of the use of SF or P(LLA-CL) in cornea tissue engineering have been reported, [32][33][34] but none about the use of blended SF/P(LLA-CL) nanofiber membranes in regeneration of the corneal endothelium.…”

mentioning

confidence: 99%

Electrospun nanofibrous SF/P(LLA-CL) membrane: a potential substratum for endothelial keratoplasty

Chen¹,

Yan²,

Zhu³

et al. 2015

IJN

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Background Cornea transplant technology has progressed markedly in recent decades, allowing surgeons to replace diseased corneal endothelium by a thin lamellar structure. A thin, transparent, biocompatible, tissue-engineered substratum with corneal endothelial cells for endothelial keratoplasty is currently of interest. Electrospinning a nanofibrous structure can simulate the extracellular matrix and have beneficial effects for cell culture. Silk fibroin (SF) has good biocompatibility but poor mechanical properties, while poly( l -lactic acid-co-ε-caprolactone) (P(LLA-CL)) has good mechanical properties but poor biocompatibility. Blending SF with P(LLA-CL) can maintain the advantages of both these materials and overcome their disadvantages. Blended electrospun nanofibrous membranes may be suitable for regeneration of the corneal endothelium. The aim of this study was to produce a tissue-engineered construct suitable for endothelial keratoplasty. Methods Five scaffolds containing different SF:P(LLA-CL) blended ratios (100:0, 75:25, 50:50, 25:75, 0:100) were manufactured. A human corneal endothelial (B4G12) cell line was cultured on the membranes. Light transmission, speed of cell adherence, cell viability (live-dead test), cell proliferation (Ki-67, BrdU staining), and cell monolayer formation were detected on membranes with the different blended ratios, and expression of some functional genes was also detected by real-time polymerase chain reaction. Results Different blended ratios of scaffolds had different light transmittance properties. The 25:75 blended ratio membrane had the best transmittance among these scaffolds. All electrospun nanofibrous membranes showed improved speed of cell adherence when compared with the control group, especially when the P(LLA-CL) ratio increased. The 25:75 blended ratio membranes also had the highest cell proliferation. B4G12 cells could form a monolayer on all scaffolds, and most functional genes were also stably expressed on all scaffolds. Only two genes showed changes in expression. Conclusion All blended ratios of SF:P(LLA-CL) scaffolds were evaluated and showed good biocompatibility for cell adherence and monolayer formation. Among them, the 25:75 blended ratio SF:P(LLA-CL) scaffold had the best transmittance and the highest cell proliferation. These attributes further the potential application of the SF:P(LLA-CL) scaffold for corneal endothelial transplantation.

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Aligned natural–synthetic polyblend nanofibers for peripheral nerve regeneration

Cited by 165 publications

References 37 publications

A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery

Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush

Electrospun nanofibrous SF/P(LLA-CL) membrane: a potential substratum for endothelial keratoplasty

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