Alkaline haematin D-575, a new tool for the determination of haemoglobin as an alternative to the cyanhaemiglobin method. I. description of the method

Zander, R.; Lang, Werner; Wolf, H. U.

doi:10.1016/0009-8981(84)90250-x

Cited by 82 publications

(51 citation statements)

References 15 publications

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“…All haemoglobin derivatives are converted into a stable end-product, alkaline haematin D-575, whose absorption maximum is at X = 575 nm and measurement can be taken on colorimeter. 2,3,5,6 The concentration of Triton X-100 can be varied over the range 25-50 g/l and that of sodium hydroxide over the range 0.01-0.1 mmol/l without altering the test results. Thus the AHD reagent can be prepared in laboratories.…”

Section: Resultsmentioning

confidence: 99%

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Evaluation of hemoglobin estimation with non-cyanide alkaline haematin D- 575 method

Anchinmane¹,

Sankhe²

2016

Int J Res Med Sci

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Section: Resultsmentioning

confidence: 99%

“…Also, the total cost of the AHD-575 method significantly lower the reagents used in HiCN method. 5 Now days, AHD-575 "ready to use" reagents are commercially available and have storage stability of upto 45 degree Celsius and for more than three years.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of hemoglobin estimation with non-cyanide alkaline haematin D- 575 method

Anchinmane¹,

Sankhe²

2016

Int J Res Med Sci

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“…Afterwards, from the hemolysate that was obtained, two measurements were conducted with the autoanalyzer, including total hemoglobin and HbA1c. The hemoglobin level was measured with the method described by Zander et al (12). HbA1c microparticle agglutination was immunoturbidimetrically measured with the inhibition method.…”

Section: Immunoturbidimetric Methodmentioning

confidence: 99%

Comparing the HbA1c Assay Results of Architect C 8000 and MQ-2000PT

Tekçe¹,

Tekçe²,

Aktas³

et al. 2015

JAREM

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Objective: Changes in a device and the modification of measurement methods are frequent issues in medical laboratories. The effects of such modifications on assay results should be investigated. HbA1c is a widely used analyte in treatment and in the follow-up of diabetic patients. We aimed to evaluate the effects of two different assay methods on the detection of the percentage of HbA1c. Methods:We used blood samples with K3-EDTA of 57 diabetic patients who were admitted to our laboratories for the HbA1c assay. HbA1c assays were performed using immunoturbidimetric (Architect C 8000; Abbot Laboratories Inc., Middletown, USA) and ion exchange chromatography (MQ-2000PT; Shanghai Hui Zhong Medical Technology Co. Ltd., Shangai, China) methods. HbA1c assays were repeated two times in both devices. Results were analyzed using MedCalc software.Results: Mean HbA1c level in immunoturbidimetric and ion exchange chromatography assays were 6.6 (min:4.1 and max:11.4) and 6.9 (min:4.9 and max:11.8), respectively. In the linear regression analysis, we detected an r value of 0.9533 (r<0.975). In Passing-Bablock analysis, we found the following equation, y=0.4+1.0 x (intercept CI:−0.22−0.68; slope CI:0.97−1.09). We did not observe any constant or proportional systematic errors between the assay methods. We found a 0.37 difference between the two methods in the Bland-Altman graphs of mean HbA1c measurements (Bias 5.7%). Conclusion:Researches on the harmonization of HbA1c are still increasing worldwide. However, at present, there are variations in methods and devices. NGSP suggests that the difference between methods should not exceed HbA1c±0.70. We found that mean HbA1c results were higher by 0.37 times in ion exchange chromatography assay compared with those in immunoturbidimetric assay. This difference is within the range suggested by NGSP. (JAREM 2015; 5: 52-5)

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“…Os níveis séricos de glicose foram determinados pelo método enzimático de referência com hexoquinase, tendo como valores referenciais glicemia de jejum entre 70 e 100 mg/dL e pós-prandial menores que 140 mg/dL (20). Para dosagem de hemoglobina glicosilada sérica, foi utilizado o método de inibição turbimétrica (TINIA), sendo os valores referenciais do mé-todo entre 4,8% e 5,9% (21). Para a determinação da frutosamina, foi utilizado teste colorimétrico por reação …”

Section: Pacientes E Métodosunclassified

Avaliação da estatura final atingida por pacientes com diabetes melito tipo 1 acompanhados em serviço terciário

Oliveira

Ferreira

Palhares

et al. 2010

Arq Bras Endocrinol Metab

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As alterações metabólicas decorrentes do diabetes melito tipo 1 (DM1) podem prejudicar o crescimento e o desenvolvimento. OBJETIVO: Avaliar a estatura final de pacientes com DM1 e a interferência da doença no crescimento. PACIENTES E MÉTODOS: A estatura final de 58 pacientes com DM1 foi avaliada em relação aos dados antropométricos e controle metabólico. RESULTADOS: A idade média foi de 26,4 anos (15,0-51,0) e a duração da doença, de 9,6 anos (1,4-25,0). A estatura final foi 165,1 cm (149,3-183,0) e o z-escore da estatura final foi igual a 0,09 (-1,76-2,85). Num subgrupo (n = 34), foi possível avaliar o controle glicêmico durante a fase de crescimento estatural. A média de hemoglobina glicosilada foi 12,2% (6,8-24,1) e as correlações entre dados antropométricos e controle glicêmico não foram estatisticamente significantes (p > 0,05). CONCLUSÃO: Não houve interferência do DM1 na estatura final, apesar do controle glicêmico inadequado.

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Alkaline haematin D-575, a new tool for the determination of haemoglobin as an alternative to the cyanhaemiglobin method. I. description of the method

Cited by 82 publications

References 15 publications

Evaluation of hemoglobin estimation with non-cyanide alkaline haematin D- 575 method

Evaluation of hemoglobin estimation with non-cyanide alkaline haematin D- 575 method

Comparing the HbA1c Assay Results of Architect C 8000 and MQ-2000PT

Avaliação da estatura final atingida por pacientes com diabetes melito tipo 1 acompanhados em serviço terciário

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