Alkaloids as chemotaxonomic markers from the Philippine endemic Uncaria perrottetii and Uncaria lanosa f. philippinensis

Olivar, Jay Edneil C.; Sy, Kurt A.; Villanueva, Christian V.; Alejandro, Grecebio Jonathan D.; Tan, Mario A.

doi:10.1016/j.jksus.2017.12.008

Cited by 14 publications

(6 citation statements)

References 17 publications

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“…The positive results of alkaloid showed an agreement with literature which stated that Uncaria genus was known for their alkaloid constituents [4,29,30]. According to Wardhani et al [31], ethanol can be used to extracted the alkaloid from kemuning leaf.…”

Section: Phytochemicals Screeningsupporting

confidence: 85%

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Syafriana¹,

Setyaningsih²,

Rachmawani³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Akar kaik-kaik (Uncaria cordata) or known as hook vine is one of the medicinal plants whose leaves are used in traditional medicine as an antidiabetic by the people of Riau. Other than that, this plant also has potential as an antimicrobial agent due to the dominant of alkaloid content possessed by members of the genus Uncaria. This research aims to study the antimicrobial activity from the ethanol extract of akar kaik-kaik leaves against Staphylococcus aureus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria), and Candida albicans (yeast). The extraction was carried out using the maceration method and a solvent of 70% ethanol. The extract then tested for phytochemical screening to find out the secondary metabolite. After that, the extract was tested for antimicrobial activity. The test was done by Kirby-Bauer Method at Nutrient Agar (NA) for bacteria, and Sabouraud Dextrose Agar (SDA) for yeast. The extract contained alkaloid, saponin, and tannin according to the phytochemical analysis. The antimicrobial activity showed that the extract can inhibit the growth of S. aureus at a concentration of 10%, 20%, and 40% respectively about 6.91 ± 0.04 mm, 8.51 ± 0.14 mm, and 10.89 ± 1.09 mm. Meanwhile, the extract cannot inhibit the growth of E. coli and C. albicans.

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Section: Phytochemicals Screeningsupporting

confidence: 85%

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Syafriana¹,

Setyaningsih²,

Rachmawani³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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“…In the continuous search for potential neuroprotective natural products against AD (Tan et al 2019(Tan et al , 2020a, the alkaloids isomitraphylline and mitraphylline (Fig. 1) from previous purifications from U. perrottetii (Olivar et al 2018) were investigated. These two alkaloids were abundant in the genus Uncaria.…”

Section: Resultsmentioning

confidence: 99%

“…The extraction of U. perrottetii leaves and the isolation and identification of the alkaloids from the U. perrottetii crude base extract have been previously reported (Olivar et al 2018). Isomitraphylline (45 mg) and mitraphylline (38 mg) were the predominant alkaloids isolated from the crude base extract based on the afforded weights.…”

Section: General Considerationsmentioning

confidence: 87%

Neuroprotective potential of the oxindole alkaloids isomitraphylline and mitraphylline in human neuroblastoma SH-SY5Y cells

Tan

2020

3 Biotech

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The purified oxindole alkaloids, isomitraphylline and mitraphylline from Uncaria perrottetii, revealed their ability to break amyloid aggregates in vitro suggesting their therapeutic potentials in Alzheimer's disease (AD). Thioflavin-T assay for assessing amyloid-beta (Aβ) aggregation of these alkaloids exhibited inhibitions at 60.321% ± 2.61 (50 μM) for isomitraphylline and 43.17% ± 3.48 (50 μM) for mitraphylline. Neuroprotective effects were elaborated against Aβ-induced SH-SY5Y cells at 20 μM and 10 μM for isomitraphylline, and 20 μM for mitraphylline. In addition, both alkaloids attenuated and protected the H 2 O 2 -induced SH-SY5Y cell cytotoxicity at 20 μM. The intracellular ROS levels of SH-SY5Y cells from H 2 O 2 -induced oxidative stress were reduced at 20 μM and 10 μM, and the mitochondrial membrane potentials of Aβ-induced SH-SY5Y cells were protected at 20 μM. The overall results suggested the potentials of both alkaloids to target certain pathological biomarkers of AD and could be further investigated as therapeutic or preventive drug leads against AD.

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“…It has also been reported that it is isolated from Mitragyna speciosa Korth. ( Beckett et al, 1965 ), Uncaria lanosa f. philippinensis (Elmer) Ridsdale ( Olivar et al, 2018 ), Uncaria bernaysii F. Muell. ( Phillipson and Hemingway, 1973 ), and Uncaria attenuata Korth.…”

Section: Natural Products Against Sars-cov-2: Computational To Preclinical Studiesmentioning

confidence: 99%

Natural Products for the Prevention and Control of the COVID-19 Pandemic: Sustainable Bioresources

Singla

Chopra

et al. 2021

Front. Pharmacol.

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Background: The world has been unprecedentedly hit by a global pandemic which broke the record of deadly pandemics that faced humanity ever since its existence. Even kids are well-versed in the terminologies and basics of the SARS-CoV-2 virus and COVID-19 now. The vaccination program has been successfully launched in various countries, given that the huge global population of concern is still far behind to be vaccinated. Furthermore, the scarcity of any potential drug against the COVID-19-causing virus forces scientists and clinicians to search for alternative and complementary medicines on a war-footing basis.Aims and Objectives: The present review aims to cover and analyze the etiology and epidemiology of COVID-19, the role of intestinal microbiota and pro-inflammatory markers, and most importantly, the natural products to combat this deadly SARS-CoV-2 virus.Methods: A primary literature search was conducted through PubMed and Google Scholar using relevant keywords. Natural products were searched from January 2020 to November 2020. No timeline limit has been imposed on the search for the biological sources of those phytochemicals. Interactive mapping has been done to analyze the multi-modal and multi-target sources.Results and Discussion: The intestinal microbiota and the pro-inflammatory markers that can serve the prognosis, diagnosis, and treatment of COVID-19 were discussed. The literature search resulted in yielding 70 phytochemicals and ten polyherbal formulations which were scientifically analyzed against the SARS-CoV-2 virus and its targets and found significant. Retrospective analyses led to provide information about 165 biological sources that can also be screened if not done earlier.Conclusion: The interactive analysis mapping of biological sources with phytochemicals and targets as well as that of phytochemical class with phytochemicals and COVID-19 targets yielded insights into the multitarget and multimodal evidence-based complementary medicines.

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Alkaloids as chemotaxonomic markers from the Philippine endemic Uncaria perrottetii and Uncaria lanosa f. philippinensis

Cited by 14 publications

References 17 publications

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Antimicrobial Activity of Ethanol Extract of Akar Kaik-kaik (Uncaria cordata (Lour.) Merr.) Leaves Against Staphylococcus aureus, Escherichia coli, and Candida albicans

Neuroprotective potential of the oxindole alkaloids isomitraphylline and mitraphylline in human neuroblastoma SH-SY5Y cells

Natural Products for the Prevention and Control of the COVID-19 Pandemic: Sustainable Bioresources

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