All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring

Ameri, Shideh Kabiri; Singh, Pramod K.; Dokmeci, Mehmet R.; Khademhosseini, Ali; Xu, Qiaobing; Sonkusale, Sameer

doi:10.1016/j.bios.2013.11.031

Cited by 34 publications

(23 citation statements)

References 35 publications

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“…DEP has been implemented for the separation of S. cerevisiae and Lactobacillus casei, and L. casei and Jurkat cells [121]. In a recent study, EK methods have been utilized for trapping and lysis of RBC [122]. AC-field was utilized for trapping, and DC-field was utilized for the lysis.…”

Section: Applicationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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Section: Applicationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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“…This makes 3D graphene a promising candidate for biological applications. [26][27][28] However, for some applications, physical confi nement of the ionic liquid is required for stable operation. In this work, we have addressed this issue by using ionic liquids as an additive to a polymer framework forming an ionic liquid-based gel electrolyte, or ionogel, as a gate to provide physical stability of the liquid dielectric.…”

Section: Introductionmentioning

confidence: 99%

Flexible 3D Graphene Transistors with Ionogel Dielectric for Low‐Voltage Operation and High Current Carrying Capacity

Ameri

Singh

D’Angelo

et al. 2016

Adv Elect Materials

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In this paper, an ionogel‐gated flexible 3D graphene transistor made from graphene foam, consisting of a network of few layers graphene, is reported. The presented transistor, fabricated on thin, 28 μm parylene, flexible substrate, demonstrates low‐voltage operation (≤2.5 V) and exhibits 4.78 times higher current capacity than previously reported liquid‐gated 3D graphene transistor fabricated on glass substrate. This is also 26.72 times higher current capacity (93 mA at 2 V applied VD) than the equivalent long channel (≈600 μm) 2D transistor device. The high current capacity and low operating voltage of this transistor are attributed to higher surface area, 3D structure of the transistor, and the high capacitive coupling between the ionogel gate and graphene. The transistor is thoroughly characterized for I–V characteristics and evaluated for circuit functionality. As a representative example, high current capacity was demonstrated by driving an LED where brightness depends on the current level through them which was tuned using the gate bias of the 3D transistor. The proposed transistor may find application in large‐area electronics for interactive displays and for large‐area sensors in structural health monitoring.

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“…Alternatives including thermal, 4 optical, 5 acoustic, 6 mechanical, 7, 8 electrical, 9, 10 and chemical 11-13 methods have been described for on-chip cell membrane permeabilization and metabolite extraction. Improvements in microfabrication have enabled the development of an unlimited assortment of integrated structures and channel geometries for trapping and lysing cells.…”

Section: Introductionmentioning

confidence: 99%

Automated sample preparation in a microfluidic culture device for cellular metabolomics

Filla

Sanders

Filla

et al. 2016

Analyst

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Sample pretreatment in conventional cellular metabolomics entails rigorous lysis and extraction steps which increase the duration as well as limit the consistency of these experiments. We report a biomimetic cell culture microfluidic device (MFD) which is coupled with an automated system for rapid, reproducible cell lysis using a combination of electrical and chemical mechanisms. In-channel microelectrodes were created using facile fabrication methods, enabling the application of electric fields up to 1000 V/cm. Using this platform, average lysing times were 7.12 s and 3.03 s for chips with no electric fields and electric fields above 200 V/cm, respectively. Overall, the electroporation MFDs yielded a ∼10-fold improvement in lysing time over standard chemical approaches. Detection of multiple intracellular nucleotides and energy metabolites in MFD lysates was demonstrated using two different MS platforms. This work will allow for the integrated culture, automated lysis, and metabolic analysis of cells in an MFD which doubles as a biomimetic model of the vasculature.

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All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring

Cited by 34 publications

References 35 publications

Microfluidic bio-particle manipulation for biotechnology

Microfluidic bio-particle manipulation for biotechnology

Flexible 3D Graphene Transistors with Ionogel Dielectric for Low‐Voltage Operation and High Current Carrying Capacity

Automated sample preparation in a microfluidic culture device for cellular metabolomics

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