Allelism of Blast Resistance Genes in Near-Isogenic Lines of Rice

Inukai, Tsuyoshi

doi:10.1094/phyto-84-1278

Cited by 97 publications

(56 citation statements)

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“…Another example of a major allelic blast-resistance locus is provided by the Pik locus on chromosome 11, where at least five genes, Pik, Pikm, Pikh, Pikp, and Piks, have been identified (Kiyosawa 1968(Kiyosawa , 1978Inukai et al 1994;Hayashi et al 2006;Li et al 2007). The genes Pik and Pikm originated in the Chinese japonica cultivars To-To (Kiyosawa 1968) and Hokushi Tami (Kiyosawa 1978), respectively, whereas Pikh and Pikp originated in the indica cultivars Te-tep (Kiyosawa 1978) and Pusur (Kiyosawa 1969a), respectively.…”

mentioning

confidence: 99%

Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

Ashikawa

Hayashi

Yamane³

et al. 2008

Genetics

370

275

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The rice blast resistance gene Pikm was cloned by a map-based cloning strategy. High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval. Sequence analysis predicted two adjacently arranged resistance-like genes, Pikm1-TS and Pikm2-TS, within this candidate region. These genes encoded proteins with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) and were considered the most probable candidates for Pikm. However, genetic complementation analysis of transgenic lines individually carrying these two genes negated the possibility that either Pikm1-TS or Pikm2-TS alone was Pikm. Instead, it was revealed that transgenic lines carrying both of these genes expressed blast resistance. The results of the complementation analysis and an evaluation of the resistance specificity of the transgenic lines to blast isolates demonstrated that Pikm-specific resistance is conferred by cooperation of Pikm1-TS and Pikm2-TS. Although these two genes are not homologous with each other, they both contain all the conserved motifs necessary for an NBS-LRR class gene to function independently as a resistance gene.

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mentioning

confidence: 99%

Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

Ashikawa

Hayashi

Yamane³

et al. 2008

Genetics

370

275

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“…This would suggest that the entire genome structure, including the Pii(t) gene, is not identical to that of Pi3(t)/Pi5(t) and that the origin of Pii(t) may differ from that of Pi3(t)/Pi5(t). Pi3(t) is known to be tightly liked to Pii(t) and both genes show very similar resistance spectra to various rice blast isolates (Inukai et al 1994;Imbe et al 2000; S.-S. Han et al, unpublished data). Considering the similar spectrum and the nearly identical chromosomal location, it is probable that Pi3(t)/ Pi5(t) and Pii(t) are the same gene and that these three DNA sequences can be utilized as a marker of Pi3(t)/Pi5(t)/Pii(t).…”

Section: Pi5(t) Markers Are Also Dominant In Pi3(t) and Pii(t) Linesmentioning

confidence: 97%

“…Genetic analysis indicates that Pi5(t) is allelic or closely linked to Pi3(t) and that Pi3(t) is closely linked to Pii(t) (Inukai et al 1994(Inukai et al , 1996. Additional disease assays have shown that the Pii(t), Pi3(t), and Pi5(t) genes display a similar reaction when tested against 16 M. grisea isolates (Imbe et al 2000;S.-S. Han et al unpublished data).…”

Section: Introductionmentioning

confidence: 97%

“…Extensive genetic studies and quantitative trait locus (QTL) analysis using DNA markers have identified more than 20 major genes and ten QTLs in rice that give rise to resistance Wang et al 1994;Naqvi and Chattoo 1996;Yu et al 1996;Ahn et al 2000;Chauhan et al 2002;Jiang and Wang 2002;Zenbayashi et al 2002). Genes conferring resistance have been identified in the durably resistant rice cultivars Tetep, Pai-Kan-Tao (PKT), Moroberekan, 5173, and LAC23 (Mackill and Bonman 1992;Inukai et al 1994;Wang et al 1994). For example, Mackill and Bonman (1992) located the genes Pi1(t), Pi2 (t), and Pi3(t) as well as two alleles of Pi4(t) associated with broad-spectrum resistance from these durably resistant rice cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea

Lee

Hong

et al. 2004

Theor Appl Genet

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Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for marker-assisted selection (MAS). The rice Pi5(t) locus confers broad-spectrum resistance to Magnaporthe grisea, the causal agent of rice blast disease. It has been hypothesized that the Pi5(t) locus carries the same gene as that encoded by the Pi3(t) and Pii(t) loci. We developed three PCR-based dominant markers (JJ80-T3, JJ81-T3, and JJ113-T3) from three previously identified BIBAC clones-JJ80, JJ81, and JJ113-that are linked to the Pi5 (t) locus. PCR analysis of 24 monogenic lines revealed that these markers are present only in lines that carry Pi5 (t), Pi3(t), and Pii(t). PCR and DNA gel-blot analysis of candidate resistance lines using JJ80-T3, JJ81-T3, and JJ113-T3 indicated that Tetep is the likely donor of Pi5(t).Of the 184 rice varieties tested, 34 carried the JJ80-T3-, JJ81-T3-, and JJ113-T3-specific bands. Disease evaluation of those 34 varieties revealed that all conferred resistance to PO6-6. The genomic structure of three of these resistant varieties (i.e., IR72, Taebaeg, Jahyangdo) is most similar to that of Pi5(t). Our results demonstrate the usefulness of the JJ80-T3, JJ81-T3, and JJ113-T3 markers for MAS for M. grisea resistance.

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“…Resistance genes in Japanese, Chinese, and IRRI differential lines were quoted from Imbe et al [14], Kiyosawa and Ling [29], Inukai et al [30], and Kobayashi et al [31].…”

Section: Co-segregation Analysis Of Candidate Genes For Piamentioning

confidence: 99%

Characterization and fine mapping of the rice blast resistance gene Pia

Zeng

Yang

Zhao

et al. 2011

Sci. China Life Sci.

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Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars (cvs) can be achieved by pyramiding of various resistance (R) genes. Pia, carried by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carried out in a mapping population consisting of 800 F 2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes (Pia-1, Pia-2, , all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene (CRG) markers (A17, A25, A26, and A27), derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 (Pia-3) perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia. rice blast, resistance gene, Pia, fine mapping, genotype Citation:Zeng X S, Yang X F, Zhao Z H, et al. Characterization and fine mapping of the rice blast resistance gene Pia.

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Allelism of Blast Resistance Genes in Near-Isogenic Lines of Rice

Cited by 97 publications

References 0 publications

Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance

Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea

Characterization and fine mapping of the rice blast resistance gene Pia

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