Allergen- and Anti-IgE-Induced Histamine Release from Whole Blood

Abstract

The release of histamine by allergen and anti-IgE from whole blood was observed in 34 asthmatic subjects with a positive skin test to house dust. The time course of histamine release showed that the release by allergen and anti-IgE peaked after 15 min incubation. There was no significant difference in the time course of the release from whole blood between allergen and anti-IgE. Anti-IgE-induced histamine release correlated to a certain extent with the serum IgE level. Histamine release by house dust, on the other hand, correlated with the radioallergosorbent test score. A striking difference was present in the dose-response slope between allergen and anti-IgE. The maximum percent release correlated with the dose-response slope by allergen, but not by anti-IgE. The amount of histamine release induced by anti-IgE paralleled the amount of the release by house dust in the cases sensitive to the allergen; less sensitive basophils to anti-IgE were less sensitive to house dust.

Safety verification for polysorbate 20, pharmaceutical excipient for intramuscular administration, in Sprague-Dawley rats and New Zealand White rabbits

“…Furthermore, serum IgE and histamine levels were measured to determine whether allergic reactions occurred after the administration of polysorbate 20 in all SD rats. In addition to clinical signs, increased levels of serum IgE and histamine are representative indicators of an allergic reaction [36][37][38]. In the current study, allergic reactions were not observed in response to the administration of polysorbate 20, which indicated that polysorbate 20 is not an allergen in SD rats.…”

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule

“…Whole blood samples were exposed to different concentrations of the HM and anti-IgE according to the method described by Tanizaki et al 25 Ten milliliters of venous blood was drawn into a plastic syringe containing 1 mL of heparin (17 IU/mL). Aliquots of 250 mL of different concentrations of HM (dose range, 10 24 to 10 mg/mL) and anti-IgE (dose range, 10 24 to 10 23 dilutions; Immunotech, Beckmann Coulter, Marseille, France) were added to the test tubes containing 500 mL of whole blood diluted 1:4 in Tris buffer (10 mmol/L Tris, 136 mmol/L NaCl, 2.7 mmol/L KCl, 0.23 mmol/L MgCl 2 , 1.8 mmol/ L CaCl 2 , and 5.5 mmol/L glucose, pH 7.3).…”

Poor association between allergen‐specific serum immunoglobulin E levels, skin sensitivity and basophil degranulation: a study with recombinant birch pollen allergen Bet v 1 and an immunoglobulin E detection system measuring immunoglobulin E capable of binding to FcɛRI

“…The challenge of whole blood with rBet v 1 and anti-IgE as a positive control was performed in a dose-response fashion according to the method described by Tanizaki et al [16]. Ten millilitres of venous blood was drawn into a plastic syringe containing 1 mL of heparin.…”