Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase

Zou, Gen; Bao, Dapeng; Wang, Ying; Zhou, Sichi; Xiao, Meili; Yang, Zhiru; Wang, Yinmei; Zhou, Zhihua

doi:10.1016/j.biortech.2020.124119

Cited by 40 publications

(21 citation statements)

References 46 publications

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“…Agrobacterium- mediated transformation was based on previously described methods ( Wang et al, 2020 ; Zou et al, 2021 ). The mycelia of CM01 were cultured in SDB at 25°C and 150 rpm for 4 days.…”

Section: Methodsmentioning

confidence: 99%

Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris

et al. 2021

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Cordyceps militaris is an entomopathogenic fungus producing a variety of bioactive compounds. To meet the huge demand for medicinal and edible products, industrialized fermentation of mycelia and cultivation of stromata have been widely developed in China. The content of bioactive metabolites of C. militaris, such as cordycepin, is higher when cultivated on silkworm pupae than on rice or in broth. However, compared with other cultivation methods, C. militaris grows more slowly and accumulates less biomass. The hypoxic environment in pupa hemocoel is one of environmental factor which is not existed in other cultivation methods. It is suggested that hypoxia plays an important role on the growth and the synthesis of bioactive compounds in C. militaris. Here, we demonstrated that the distinct effects on the growth and synthesis of bioactive compounds employing different strategies of improving hypoxia adaption. The introduction of Vitreoscilla hemoglobin enhanced growth, biomass accumulation, and crude polysaccharides content of C. militaris. However, cordycepin production was decreased to 9–15% of the control group. Meanwhile, the yield of adenosine was increased significantly. Nonetheless, when the predicted bHLH transcription factor of sterol regulatory element binding proteins (SREBPs) was overexpressed in C. militaris to improve the hypoxia adaption of fungal cells, cordycepin content was significantly increased more than two-fold. These findings reveal the role of SREBPs on growth and bioactive compounds synthesis. And it also provides a scientific basis for rationally engineering strains and optimization strategies of air supply in cultivation and fermentation.

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Section: Methodsmentioning

confidence: 99%

Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris

et al. 2021

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“…Efficient cellulose hydrolysis is considered a complex mechanism with the synergic action of three cellulase sub‐enzymes: endoglucanase, exoglucanase, and β ‐glucosidase 14,15 . β ‐Glucosidase is not found abundantly in the T. reesei enzymatic system 16–18 . It has been suggested that the production of cellulase by T. reesei fungi could be improved through co‐culturing with another fungus 19 …”

Section: Introductionmentioning

confidence: 99%

“…14,15 β-Glucosidase is not found abundantly in the T. reesei enzymatic system. [16][17][18] It has been suggested that the production of cellulase by T. reesei fungi could be improved through co-culturing with another fungus. 19 The algal biomass is rich in organic compounds with a strong potential for efficient biofuel production due to its abundance and its availability free of charge.…”

Section: Introductionmentioning

confidence: 99%

Exploring strategies for the use of mixed microalgae in cellulase production and its application for bioethanol production

Shokrkar

Zamani

Ebrahimi

2022

Biofuels Bioprod Bioref

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Improving the enzymatic hydrolysis processes of cellulosic materials can be considered a key aim for bioethanol production. Cellulase production using mixed microalgae by mono and co-cultivation of Trichoderma reesei and Aspergillus niger has not yet been reported. In this study, the effect of different concentration ratios of cellulose and microalgae on the production of cellulases was investigated. Media containing equal mass percentages of microalgae and cellulose showed the highest cellulase activity (3.3 U mL −1 ) among other tested ratios using co-cultivation of fungi. The optimal percentage of microalgae (50%) increased enzyme activity by about 20% compared with pure cellulose due to necessary and rare elements in the microalgae. Subsequently, fermentation of this sample to bioethanol using Saccharomyces cerevisiae demonstrated a yield of 0.46 g ethanol/g glucose (92% of the theoretical value). The microalgae ratio is therefore an important factor and can be optimized to gain the highest enzyme activity and improve the cost-effectiveness of bioethanol production from microalgae.

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“…The rapidly growing global population has also resulted in growing food demand and increased agricultural output, leading to the generation of agricultural byproducts and wastes, such as sugarcane bagasse, rice husks, cotton stalks, straw, and stover [ 1 ]. Mushrooms vegetatively grow on these stubborn residual biomasses and convert them into high-value edible and medicinal products [ 2 , 3 , 4 ]. In China, the economic output of edible fungi has ranked as the fifth-largest agricultural sector after grain, vegetables, fruit trees, and oilseeds, ahead of tea, sugar, and cotton [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation in Hypsizygus marmoreus

Gong

Huang

et al. 2022

JoF

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Hypsizygus marmoreus is a representative edible mushroom with low-temperature fruiting after a long postripening (LFLP). Clarifying the mechanism of LFLP and applying a rigorous low-temperature-limited process will optimize the mushroom cultivation process. This study performed an integrative multi-omics analysis of the molecular mechanism of LFLP in combination with genetic, physiological, and cultivation confirmation. The results showed that the amino acid content was increased during LFLP, mainly arginine. pH analysis showed acidification in the postripening stage and alkalization in the substrates of the reproductive growth stage. An enzyme activity test confirmed the increased enzyme activity of arginase and citrate synthase in the postripening stage. Weighted gene coexpression network analysis of the transcriptome and metabolomics indicated that pH variation is correlated mainly with changes in citrate and arginine. Multi-omics reveals a straightforward way of providing enriched materials for amino acid biosynthesis, namely, synergistically elevating citric acid and arginine through enhanced activity of the arginine synthesis branch pathway in the citrate cycle. Our study confirmed that GCN2 mediated metabolic adaptation by enhancing protein translation, highlighting its regulatory role during LFLP. Exogenously added citric acid and arginine shortened the postripening period by 10 days and increased the fruiting body yield by 10.2~15.5%. This research sheds light on the molecular mechanism of LFLP in H. marmoreus and highlights the promising application of nutrient accumulation in high-efficiency cultivation.

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Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase

Cited by 40 publications

References 46 publications

Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris

Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris

Exploring strategies for the use of mixed microalgae in cellulase production and its application for bioethanol production

Multi-Omics Analysis of Low-Temperature Fruiting Highlights the Promising Cultivation Application of the Nutrients Accumulation in Hypsizygus marmoreus

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