Alleviation Effects of Bifidobacterium animalis subsp. lactis XLTG11 on Dextran Sulfate Sodium-Induced Colitis in Mice

Abstract

Inflammatory bowel disease (IBD) is a chronic immune-related disease, which can occur through the dysfunction of the immune system caused by the imbalance of gut microbiota. Previous studies have reported the beneficial effects of Bifidobacterium on colitis, while the related mechanisms behind these effects have not been fully elucidated. The aim of our study is to investigate the alleviation effect of Bifidobacterium animalis subsp. lactis XLTG11 (B. lactis) on dextran sulfate sodium (DSS)-induced colitis and its potential mechanism. The results showed that B. lactis XLTG11 significantly decreased weight loss, disease activity index score, colon shortening, myeloperoxide activity, spleen weight, and colon tissue damage. Additionally, B. lactis XLTG11 significantly decreased the levels of pro-inflammatory cytokines and increased the level of anti-inflammatory cytokine. Meanwhile, high doses of B. lactis XLTG11 significantly up-regulated the expression of tight junction proteins and inhibited activation of Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MYD88)/nuclear factor-κB (NF-κB) signaling pathway. Furthermore, B. lactis XLTG11 increased the gut microbiota diversity and modulated gut microbiota composition caused by DSS. Moreover, Spearman’s correlation analysis also found that several specific gut microbiota were significantly correlated with colitis-related indicators. These results demonstrated that B. lactis XLTG11 can alleviate DSS-induced colitis by inhibiting the activation of the TLR4/MYD88/NF-κB signaling pathway, regulating inflammatory cytokines, improving intestinal barrier function, and modulating the gut microbiota.

Bifidobacterium BLa80 mitigates colitis by altering gut microbiota and alleviating inflammation

“…We further observed a positive modulatory effect of B. lactis BLa80 on the gut microbiota in mice with UC. Our results are consistent with recent findings showing that B. lactis XLTG11 can attenuate DSS-induced UC in a process associated with inhibition of signalling through the TLR4/MYD88/NF-κB pathway, modulation concentrations of inflammatory cytokines, and remodelling of the gut microbiota (Wang et al 2021 ).…”

Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model

“…In the current study, we observed that the probiotic consortia ameliorated the symptoms of mice with DSS-induced colitis, as evidenced by a reduction in shortened colon length, weight loss, DAI score, mucosal loss, and inflammatory cell infiltration, which are in accordance with previous studies ( 37 , 49 – 51 ). Additionally, IHC revealed a reduction in F4/80 + macrophages in the probiotic consortia group.…”

Bifidobacterium animalis subsp. lactis XLTG11 improves antibiotic-related diarrhea by alleviating inflammation, enhancing intestinal barrier function and regulating intestinal flora

“…lactis XLTG11 could adjust the composition of the intestinal flora and significantly alleviate gastrointestinal related diseases. 16…”

“…lactis XLTG11 could adjust the composition of the intestinal flora and significantly alleviate gastrointestinal related diseases. 16 Ampicillin is a widely used kind of sesgalactosamine. It has an inhibitory effect on a variety of Gram-positive and Gramnegative bacteria, and has broad-spectrum activity against various bacterial groups.…”