Aloin reduces inflammatory gene iNOS via inhibition activity and p-STAT-1 and NF-κB

Lee, Wonhwa; Yang, Sumin; Lee, Changhun; Park, Eui Kyun; Kim, Kyung–Min; Ku, Sae‐Kwang; Bae, Jong‐Sup

doi:10.1016/j.fct.2019.02.025

Cited by 45 publications

(22 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the non-cancerous cell line RAW264.7, phosphorylation of STAT1, STAT3, JAK1 and JAK2 were not affected by aloin treatment (83). In addition, aloin did not affect STAT1 phosphorylation in HUVECs (128).…”

Section: Janus Kinase (Jak)/ Signal Transducer and Activator Of Transcription (Stat) Pathwaymentioning

confidence: 87%

Molecular mechanisms and associated cell signalling pathways underlying the anticancer properties of phytochemical compounds from Aloe species (Review)

2021

View full text Add to dashboard Cite

Naturally occurring components from various species of Aloe have been used as traditional folk medicine since the ancient times. Over the last few decades, the therapeutic effects of extracts and phytochemical compounds obtained from Aloe vera have been proven in preclinical and clinical studies. Recently, compounds from other Aloe species apart from Aloe vera have been investigated for the treatment of different diseases, with a particular focus on cancer. In the present review, the effects of phytochemical compounds obtained from different Aloe species are discussed, with a specific focus on the effects on cell signalling in cancer and normal cells, and their selectivity and efficacy. This information will be useful for the application of Aloe-derived compounds as therapeutic agents, either alone or in combination with other standard drugs for cancer treatment. Contents 1. Introduction 2. Cell death initiation by the phytochemical compounds of Aloe 3. Anticancer selectivity 4. Cell cycle regulation 5. Cell signalling 6. Angiogenesis 7. Migration and invasion 8. Inflammation and immunomodulation (TNF-α/NF-κB pathway) 9. Recommendations for future applications 10. Conclusions

show abstract

Section: Janus Kinase (Jak)/ Signal Transducer and Activator Of Transcription (Stat) Pathwaymentioning

confidence: 87%

Molecular mechanisms and associated cell signalling pathways underlying the anticancer properties of phytochemical compounds from Aloe species (Review)

2021

View full text Add to dashboard Cite

show abstract

“…When TLR4 was knocked down in the H/R model of primary hepatocytes, the protein expression levels of TLR4, MyD88, TRAF6, p-IKK α/β , and p-NF- κ B p65 were also inhibited by AL (Figure 7(c)). Moreover, AL reduces inflammatory gene iNOS via the inhibition of the activation of NF- κ B in a model of LPS-induced lung injury [34, 45]. Luo confirmed that AL suppresses LPS-induced inflammatory response and apoptosis by inhibiting the activation of NF- κ B in RAW 264.7 cells [11].…”

Section: Discussionmentioning

confidence: 98%

Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal PathwayIn VivoandIn Vitro

Qian

Gao

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Background Aloin exerts considerable protective effects in various disease models, and its effect on hepatic ischemia-reperfusion (HIR) injury remains unknown. This research is aimed at conducting an in-depth investigation of the antioxidant, anti-inflammatory, and antiapoptosis effects of aloin in HIR injury and explain the underlying molecular mechanisms. Methods In vivo, different concentrations of aloin were intraperitoneally injected 1 h before the establishment of the HIR model in male mice. The hepatic function, pathological status, oxidative stress, and inflammatory and apoptosis markers were measured. In vitro, aloin (AL, C21H22O9) or lipopolysaccharide (LPS) was added to a culture of mouse primary hepatocytes before it underwent hypoxia/reoxygenation (H/R), and the apoptosis in the mouse primary hepatocytes was analyzed. Results We found that 20 mg/kg was the optimum concentration of aloin for mitigating I/R-induced liver tissue damage, characterized by decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Aloin pretreatment substantially suppressed the generation of hepatic malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and IL-6 and enhanced the hepatic superoxide dismutase (SOD) activities as well as glutathione (GSH) and IL-10 levels in the liver tissue of I/R mice; this indicated that aloin ameliorated I/R-induced liver damage by reducing the oxidative stress and inflammatory response. Moreover, aloin inhibited hepatocyte apoptosis and inflammatory response that was caused by the upregulated expression of Bcl-2, the downregulated expression of cleaved caspase3(C-caspase3), Bax, Toll-like receptor 4 (TLR4), FADD, MyD88, TRAF6, phosphorylated IKKα/β (p-IKKα/β), and phosphorylated nuclear factor κB p65 (p-NF-κB p65).

show abstract

“… Compounds Structure In vitro/in vivo Model (effective dose) Cells (effective concentration) Related pharmacological indicators Related molecular mechanisms Refs. Chrysophanol 126 Paraquat (10 mg/kg) TNF-α↓ IL-1β↓ IL-6↓ MDA↓ SOD↑ Activation of PPAR-γ Inactivation of NF-κB signaling pathway [ 354 ] Emodin 127 Pancreatitis/Cigarette smoke/LPS (20 mg/kg) RAW 264.7 (20 μM) MPO↓ TNF-α↓ IL-1β↓ IL-6↓ NO↓ Inhibition of mTOR/HIF-1α/VEGF pathway Up-regulation of AQP1, AQP5 and Nrf2/HO-1 signaling pathway [ [355] , [356] , [357] , [358] , [359] , [360] , [361] ] Rhein 128 RSV (120 mg/kg) TNF-α↓ IL-1β↓ IL-6↓ IL-18↓ IL-33↓ Inhibition of NLRP3 inflammasome and NF-κB signaling pathway [ 362 ] Aloe-emodin 129 USA300 MRSA (100 mg/kg) A549/MH-S (16 μg/mL) LDH↓ Suppression of pore-forming activity of α-toxin [ 363 ] Aloin 130 LPS (12.4 mg/kg) HUVECs (100 μM) TNF-α↓IL1-β↓ iNOS↓ NO↓ COX2↓ Activation of HO-1/ Nrf2 signaling pathway Inactivation of NF-κB and STAT-1 signaling pathway [ 364 ] Shikonin 131 LPS (25 mg/kg) THP‐1/MPMs (2.5 μM) MLE‐12 (50 μg/mL) MPO↓ TNF-α↓ IL-1β↓ IL-6↓ COX-2↓ iNOS↓ ICAM-1↓ MCP-1↓ Disruption of the MD2–TLR4 complex Inhibition of MAPK and miRNA-140−5p/TLR4/MyD88/NF-κB signaling pathways [ [365] , ...…”

Section: Mechanisms Involved In Ali/ardsmentioning

confidence: 99%

“…Berg. In previous study, aloin remarkedly inhibited lung injury as well as reduced inflammatory markers (iNOS, NO, COX2, TNF-α and IL1-β) in mice and human umbilical vein endothelial cells stimulated by LPS, which were related to the activation of Nrf2/HO-1 as well as inactivation of the NF-κB and STAT-1 signaling pathways [ 364 ].…”

Section: Natural Compounds That Exert Anti-ali Effectsmentioning

confidence: 99%

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Zhou

et al. 2021

Pharmacological Research

251

121

View full text Add to dashboard Cite

show abstract

Aloin reduces inflammatory gene iNOS via inhibition activity and p-STAT-1 and NF-κB

Cited by 45 publications

References 26 publications

Molecular mechanisms and associated cell signalling pathways underlying the anticancer properties of phytochemical compounds from Aloe species (Review)

Molecular mechanisms and associated cell signalling pathways underlying the anticancer properties of phytochemical compounds from Aloe species (Review)

Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury via Inhibiting TLR4/MyD88/NF-κB Signal PathwayIn VivoandIn Vitro

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Contact Info

Product

Resources

About