Alpha Amylase Enzyme Inhibitory and Anti-inflammatory Effect of Lawsonia inermis

Abstract

Previously it was reported elsewhere that Lawsonia inermis have anti-inflammatory and analgesic effect in experimental animals. The in vitro porcine alpha amylase inhibitory effect was investigated of this plant methanolic extracts and consequently hypoglycemic effect by quantitatively determining the maltose from the maltose standard curve while the anti-inflammatory effect by acetic acid induced writhing test in mice. Acarbose (10 microg mL(-1)) and Diclofenac sodium (20 mg kg(-1)) were used as reference hypoglycemic and anti-inflammatory drugs, respectively, for this study. The methanolic leaves extract of the plant significantly inhibited (60.97% compared to untreated) enzymatic activity of the amylase at 10 microg mL(-1) dose (p < 0.05) also reduced the chemically induced nociceptive pain stimuli significantly at all doses (p < 0.01). Carbohydrates, glycosides, flavonoids, saponins and tannins were found to have in phytochemical screening of the extract which are thought to bring these effects. For the conclusive purpose, it is suggesting from the result that the pharmacological properties of this Lawsonia inermis can elicit hypoglycemic effect by inhibiting alpha-amylase enzyme and can reduce neurogenic pain stimulus. It gives the notion that how this group of patient would be therapeutically benefitted by decreasing both these effects by the same agent which is easy available.

A Review on Lawsonia Inermis: A Potential Medicinal Plant

“…The antidiabetic effect of methanolic extracts of Lawsonia inermis was determined by quantitatively determining the maltose from the maltose standard curve. The methanolic leaves extract of the plant significantly inhibited the enzymatic activity of the amylase at 10 µg/m dose (60.97% compared to untreated, p<0.05) [113].…”

“…The anti-inflammatory effect of methanolic extracts of Lawsonia inermis was determined using acetic acid-induced writhing test in mice. The methanolic leaves extract significantly reduced the chemically induced nociceptive pain stimuli (p<0.01) [113].…”

Hepatoprotective Effects of Polyherbal Formulation Against Carbon Tetrachloride-Induced Hepatic Injury in Albino Rats: A Toxicity Screening Approach

“…This plant has been used for the treatment of epilepsy and for malignant ulcer. Henna reduced both hyperglycemia and inflammation significantly [18]. Moreover, the leaves are used as hepatoprotective, antioxidant, bronchitis, headache, ophthalmia, syphilitics, amenorrhea, scabies, and also in hair growth [19][20][21][22][23][24][25].…”

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

“…Data showed that AHE contained high amounts of lawsone (2-hydroxy-1,4-naphthalenedione), dimethyl sulfoxide, eugenol, dimethyl sulfide, and maltol, which were estimated as about 2.30%, 2.29%, 1.35% 1.22%, and 0.63%, respectively. It has been reported that lawsone, the major compound of henna, particularly concentrated in the leaf, displayed several biological properties such as antioxidant [6], antibacterial [28] and anti-inflammatory, analgesic, anticancer activities [7].…”