Calotropis procera, belonging to the Asclepidaceae family, is present more or less throughout India and in other warm, dry places such as, Warizistan, Afghanistan, Egypt, and tropical Africa. Its common names are Akra, Akanal, and Madar. The leaves of Calotropis procera are said to be valuable as an antidote for snake bite, sinus fistula, rheumatism, mumps, burn injuries, and body pain. The leaves of Calotropis procera are also used to treat jaundice. A study on Calotropis procera leaf samples extracted the air-dried leaf powder with different solvents such as petroleum-ether (60-80°C), benzene, chloroform, ethanol, and sterile water. Preliminary phytochemical analysis was done long with measurement of the leaf constants, fluorescence characteristics, and extractive values. Quantitative estimation of total ash value, acid insoluble ash, and water- soluble ash may serve as useful indices for identification of the powdered drug. Histochemical studies which reveal rows of cylindrical palisade cells and, vascular bundles may also serve as useful indices for identification of the tissues. These studies suggested that the observed pharmacognostic and physiochemical parameters are of great value in quality control and formulation development of Calotropis procera.

The objective of this study was to investigate the hepatoprotective activity of ethonolic extract of polyherbalformulation (Solanumxanthocarpum, Glycyrrhizaglabra, Ricinuscommunis, and Phyllanthusniruri leaves) against carbon tetrachloride (CCl 4) induced hepatotoxicity. The plants were dried in shade then powdered, extracted with ethanol. Preliminary phytochemical tests were done with the ethanolic extract showed presence of mostly all the compounds. The hepatoprotective activity of the ethonolic extract was assessed in CCl 4 induced hepatotoxic albino rats. HPLC and UV spectrum analysis revealed to identify and to quantify each component.The present study that exhibit free radical scavenging properties. DNA damage in liver was also evaluated using Comet assay. The DNA damages and the length are tested, Progression of liver damage induced by CCl 4 in albino rats can be intervened using the polyherbal extract and these effects are comparable to those of silymarin, polyherbal treatments effectively restored these measurements closer to their normal levels.

Objective: To investigate the hepatoprotective activity of aqueous methanolic extract of polyherbal formulation and individual extracts of Calotropis procera, Gymnema sylvestre, and Lawsonia inermis leaves. Methods:The hepatoprotective activity of the formulation and extracts were assessed on the basis of histopathological changes in liver of carbon tetrachloride-induced hepatotoxic rats. Results:The result of present studies strongly indicates that the hepatoprotective property of polyherbal formulation as evidenced by less damaged hepatocytes cells. Conclusion:Polyherbal formulation revealed excellent hepatoprotective profile as compared to individual plants due to synergistic effect produced by different phytochemicals present in the formulation.

The results of the current study indicate that the substitution at pyrazole scaffold could improve anti-inflammatory activity.

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