Alteration of adipose tissue immune cell milieu towards the suppression of inflammation in high fat diet fed mice by flaxseed oil supplementation

Bashir, Samina; Sharma, Yadhu; Jairajpuri, Deeba Shamim; Rashid, Faraz; Nematullah, Md; Khan, Farah

doi:10.1371/journal.pone.0223070

Cited by 15 publications

(11 citation statements)

References 33 publications

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“…A downregulation of ARG1 expression was observed in the 14:1 group as opposed to the STD group ( p < 0.001). Similar results were obtained in the study of Bashir et al where ARG1 protein and mRNA levels were reduced in murine macrophages in adipose tissue of the group fed an HFD in comparison to the lean group fed standard chow [ 59 ]. L-Arginine (Arg), hydrolyzed by arginase, is considered an important amino acid in homeostasis maintenance, and its metabolism is altered in various diseases [ 60 ].…”

Section: Discussionsupporting

confidence: 89%

Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

et al. 2021

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The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n–6) to α-linolenic acid (ALA, C18:3n–3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB—Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.

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Section: Discussionsupporting

confidence: 89%

Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

et al. 2021

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“…In this study, the HFD group displayed significantly increased body, heart, liver, kidney, and spleen weights compared to the control group. This result is congruous with a previous study (Bashir et al, 2019). As a result, increasing fat intake can lead to increased energy consumption, energy density, and palatability.…”

Section: Discussionsupporting

confidence: 78%

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Seliem

Azab

Ismaila

et al. 2022

Benha Veterinary Medical Journal

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The present work was performed to elucidate the effect of linseed oil supplementation on metabolic disorders in induced obese male albino rats. Thirty male Albino Wister rats were used in this investigation. They were categorized into two groups; 10 rats received a controldiet, and 20 rats were supplied a high-fat diet for two months till the induction of obesity. Then, rats were split into 3 groups (10 rats each). Group I was maintained on a control diet. Group II was maintained on HFD only. group III received HFD with 30% linseed oil for further two months. At the end of the experiment, the rat's body and organs weights were assessed. Blood samples and adipose tissue were obtained for analysis. The results revealed that linseed oil supplementation caused a substantial (P < 0.05) reduction in serum TC, TAG, LDL-C, VLDL-C, glucose, insulin levels, and HOMA-IR. However, HDL-C and adiponectin levels showed a significant (P < 0.05) increase when compared with the HFD group. Linseed oil supplementation also caused significant down regulation of retinol-binding protein 4 (RBP4) gene expression, however, the GLUT4 gene showed significant upregulation. From the obtained results it could be concluded that linseed oil has an ameliorative effect on altered lipid profile caused by obesity. In addition, linseed oil caused improvement of insulin resistance in addition to the pro and anti-inflammatory biomarkers GLUT4 Insulin resistance Linseed oil Obesity RBP4

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“…There is evidence that obesity generally drives a unique signature of SPM deficiency (19,(30)(31)(32)(33)(34)(35)(36)(37). Table 1 summarizes the results of these studies.…”

Section: Obesity Promotes a Signature Of Spm Deficiencymentioning

confidence: 99%

Obesity-Driven Deficiencies of Specialized Pro-resolving Mediators May Drive Adverse Outcomes During SARS-CoV-2 Infection

et al. 2020

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Obesity is a major independent risk factor for increased morbidity and mortality upon infection with Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2), which is responsible for the current coronavirus disease pandemic (COVID-19). Therefore, there is a critical need to identify underlying metabolic factors associated with obesity that could be contributing toward increased susceptibility to SARS-CoV-2 in this vulnerable population. Here, we focus on the critical role of potent endogenous lipid metabolites known as specialized pro-resolving mediators (SPMs) that are synthesized from polyunsaturated fatty acids. SPMs are generated during the transition of inflammation to resolution and have a vital role in directing damaged tissues to homeostasis; furthermore, SPMs display anti-viral activity in the context of influenza infection without being immunosuppressive. We cover evidence from rodent and human studies to show that obesity, and its co-morbidities, induce a signature of SPM deficiency across immunometabolic tissues. We further discuss how the effects of obesity upon SARS-CoV-2 infection are likely exacerbated with environmental exposures that promote chronic pulmonary inflammation and augment SPM deficits. Finally, we highlight potential approaches to overcome the loss of SPMs using dietary and pharmacological interventions. Collectively, this mini-review underscores the need for mechanistic studies on how SPM deficiencies driven by obesity and environmental exposures may exacerbate the response to SARS-CoV-2.

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Alteration of adipose tissue immune cell milieu towards the suppression of inflammation in high fat diet fed mice by flaxseed oil supplementation

Cited by 15 publications

References 33 publications

Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Obesity-Driven Deficiencies of Specialized Pro-resolving Mediators May Drive Adverse Outcomes During SARS-CoV-2 Infection

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