Alterations in DNA methylation profiles in cancellous bone of postmenopausal women with osteoporosis

Yu, Zhe; Yang, Ling; Hong, Wang; Chen, Xi; Jiang, Wei; Wang, Zhicong; Liu, Shuping; Liu, Yuehong

doi:10.1002/2211-5463.12907

Cited by 20 publications

(16 citation statements)

References 40 publications

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“…Finally, given the important role of cancellous bone in bone development [ 24 ], the research also focused on EWAS in this tissue derived from patients with postmenopausal osteoporosis. This microarray-based analysis identified 13 genes differently methylated in OP patients, reported in Table 1 to be involved in five important bone-related signaling pathways (calcium; Cyclic Guanosine Phospho-Protein Kinase G, cGMP-PKG; endocytosis; Ras-Associated Protein-1, Rap1; Adenosine Activated Protein Kinase, AMPK) [ 25 ]. Undoubtedly, genome-wide studies represent an invaluable tool in understanding epigenetic mechanisms, although functional validation are needed to confirm novel identified DNA methylation patterns that may contribute to OP development.…”

Section: Dna Methylation Pattern In Osteoporosismentioning

confidence: 99%

DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review

Visconti

Cariati

Fittipaldi

et al. 2021

IJMS

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DNA methylation is one of the most studied epigenetic mechanisms that play a pivotal role in regulating gene expression. The epigenetic component is strongly involved in aging-bone diseases, such as osteoporosis and osteoarthritis. Both are complex multi-factorial late-onset disorders that represent a globally widespread health problem, highlighting a crucial point of investigations in many scientific studies. In recent years, new findings on the role of DNA methylation in the pathogenesis of aging-bone diseases have emerged. The aim of this systematic review is to update knowledge in the field of DNA methylation associated with osteoporosis and osteoarthritis, focusing on the specific tissues involved in both pathological conditions.

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Section: Dna Methylation Pattern In Osteoporosismentioning

confidence: 99%

DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review

Visconti

Cariati

Fittipaldi

et al. 2021

IJMS

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show abstract

“…Cancellous bone can be affected by osteoporosis earlier than in cortical bone, and a DNA methylation microarray study of the hip cancellous bone in osteoarthritis patients has demonstrated differential methylation [ 44 ]. Zhou et al reported five crucial signaling pathways recently, namely, the signaling pathways of calcium, endocytosis, cyclic guanosine phospho-protein kinase G (cGMPPKG), Rap1, and 5’ adenosine monophosphate-activated protein kinase (AMPK).…”

Section: Dna Methylationmentioning

confidence: 99%

“…Zhou et al reported five crucial signaling pathways recently, namely, the signaling pathways of calcium, endocytosis, cyclic guanosine phospho-protein kinase G (cGMPPKG), Rap1, and 5’ adenosine monophosphate-activated protein kinase (AMPK). All were involved in cancellous weakness in postmenopausal women [ 44 ].…”

Section: Dna Methylationmentioning

confidence: 99%

The Role of Epigenomics in Osteoporosis and Osteoporotic Vertebral Fracture

Kim

Lee

Han

2020

IJMS

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Osteoporosis is a complex multifactorial condition of the musculoskeletal system. Osteoporosis and osteoporotic vertebral fracture (OVF) are associated with high medical costs and can lead to poor quality of life. Genetic factors are important in determining bone mass and structure, as well as any predisposition for bone degradation and OVF. However, genetic factors are not enough to explain osteoporosis development and OVF occurrence. Epigenetics describes a mechanism for controlling gene expression and cellular processes without altering DNA sequences. The main mechanisms in epigenetics are DNA methylation, histone modifications, and non-coding RNAs (ncRNAs). Recently, alterations in epigenetic mechanisms and their activity have been associated with osteoporosis and OVF. Here, we review emerging evidence that epigenetics contributes to the machinery that can alter DNA structure, gene expression, and cellular differentiation during physiological and pathological bone remodeling. A progressive understanding of normal bone metabolism and the role of epigenetic mechanisms in multifactorial osteopathy can help us better understand the etiology of the disease and convert this information into clinical practice. A deep understanding of these mechanisms will help in properly coordinating future individual treatments of osteoporosis and OVF.

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“…miRNAs participate in osteoporosis research by regulating target genes [15]. On the other hand, some gene methylation is also associated with osteoporosis [16]. Studies speculate that methylation modification of genes in osteoporotic patients may be a compensatory mechanism to combat osteoporosis-related bone loss [17].…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Therapeutic Targets and Molecular Regulatory Mechanisms for Osteoporosis by Bioinformatics Methods

Zhang

Yang

Geng

et al. 2021

BioMed Research International

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Background. Osteoporosis is characterized by low bone mass, deterioration of bone tissue structure, and susceptibility to fracture. New and more suitable therapeutic targets need to be discovered. Methods. We collected osteoporosis-related datasets (GSE56815, GSE99624, and GSE63446). The methylation markers were obtained by differential analysis. Degree, DMNC, MCC, and MNC plug-ins were used to screen the important methylation markers in PPI network, then enrichment analysis was performed. ROC curve was used to evaluate the diagnostic effect of osteoporosis. In addition, we evaluated the difference in immune cell infiltration between osteoporotic patients and control by ssGSEA. Finally, differential miRNAs in osteoporosis were used to predict the regulators of key methylation markers. Results. A total of 2351 differentially expressed genes and 5246 differentially methylated positions were obtained between osteoporotic patients and controls. We identified 19 methylation markers by PPI network. They were mainly involved in biological functions and signaling pathways such as apoptosis and immune inflammation. HIST1H3G, MAP3K5, NOP2, OXA1L, and ZFPM2 with higher AUC values were considered key methylation markers. There were significant differences in immune cell infiltration between osteoporotic patients and controls, especially dendritic cells and natural killer cells. The correlation between MAP3K5 and immune cells was high, and its differential expression was also validated by other two datasets. In addition, NOP2 was predicted to be regulated by differentially expressed hsa-miR-3130-5p. Conclusion. Our efforts aim to provide new methylation markers as therapeutic targets for osteoporosis to better treat osteoporosis in the future.

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Alterations in DNA methylation profiles in cancellous bone of postmenopausal women with osteoporosis

Cited by 20 publications

References 40 publications

DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review

DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review

The Role of Epigenomics in Osteoporosis and Osteoporotic Vertebral Fracture

Identification of Potential Therapeutic Targets and Molecular Regulatory Mechanisms for Osteoporosis by Bioinformatics Methods

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