Alterations in Leukocyte Function following Surgical Trauma: Differentiation of Distinct Reaction Types and Association with Tumor Necrosis Factor Gene Polymorphisms

Majetschak, Matthias; Krehmeier, Ulrich; Ostroverkh, Liljana; Blömeke, Brunhilde; Schäfer, Michael K. E.

doi:10.1128/cdli.12.2.296-303.2005

Cited by 11 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several previous studies have investigated the influence of shock, (surgical) trauma and sepsis on the ability of peripheral leucocytes to produce pro‐inflammatory mediators upon LPS stimulus . However, those studies either used isolated cells, disregarding the role of fluid‐phase mediators, granulocytes, and platelets, or employed stimulation protocols which are not feasible in clinical routine.…”

Section: Discussionmentioning

confidence: 99%

Functional immune monitoring in severely injured patients—A pilot study

et al. 2019

View full text Add to dashboard Cite

After severe trauma, the resulting excessive inflammatory response is countered by compensatory anti‐inflammatory mechanisms. The systemic inflammatory response to trauma enhanced by inappropriately timed surgical second hits may be detrimental for the patient. On the other hand, overwhelming anti‐inflammatory mechanisms may put patients at increased risk from secondary local and systemic infections. The ensuing sepsis and organ dysfunction due to immune dysregulation remain the leading causes of death after injury. To date, there are no clinically applicable techniques to monitor the pro‐/anti‐inflammatory immune status of the patients and the remaining ability to react to microbial stimuli. Therefore, in the present study, we used a highly standardized and easy‐to‐use system to draw peripheral whole blood from polytraumatized patients (ISS ≥ 32, n = 7) and to challenge it with bacterial lipopolysaccharide. Secreted cytokines were compared with those in samples from healthy volunteers. We observed a significant decrease in the release of monocyte‐derived mediators. Surprisingly, we detected stable or even increased concentrations of cytokines related to T cell maturation and function. For clinical practicability, we reduced the incubation time before supernatants were collected. Even after an abbreviated stimulation period, a stable release of almost all analysed parameters in patient blood could be detected. In conclusion, the data are indicative of a clinically well‐applicable approach to monitor the immune status in severely injured patients in a short time. This may be used to optimize the timing of necessary surgical interventions to avoid a boost of proinflammation and reduce risk of secondary infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional immune monitoring in severely injured patients—A pilot study

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Despite advancements in prehospital care, early balanced resuscitation in the emergency department (ED), and damage control surgery, the mortality rate among THS is still high (4). The outcome of patients with THS is determined by the severity of injury, altered immune cell functions, hormonal status, and secreted cytokines (5,6). The inflammatory response is essential to host defense against infections, but inflammatory disparity caused by imbalance production of inflammatory and anti-inflammatory cytokines may lead to MODS and poor clinical outcomes (7,8).…”

Section: Introductionmentioning

confidence: 99%

Estrogen as a Safe Therapeutic Adjunct in Reducing the Inflammatory Storm in Trauma Hemorrhagic Shock Patients

Gupta

Tiwari

Sinha

et al. 2021

Shock

View full text Add to dashboard Cite

Trauma is a major cause of death and disability throughout the world. It is a leading cause of death with or without sepsis in about 50% of patients. Limited therapeutic options are available besides definitive care with a mortality benefit. Preclinical studies have demonstrated the mortality benefit of estrogen in trauma hemorrhagic shock (THS). Based on encouraging results from preclinical studies, we hypothesized that early administration of estrogen in male THS patients may reduce the inflammatory storm, prevent sepsis-associated problems, and subsequently reduce mortality. The authors studied the safety of early administration of estrogen as a therapeutic adjunct in the emergency department (ED) and its effects on the inflammatory storm, prevention of sepsis, and mortality during the intensive care unit stay. Forty THS patients were recruited. THS patients were divided into experimental and placebo control groups based on the estrogen administration in the ED. Serum levels of cytokines and immune cells were measured at different time points on days 0, 3, 7, and 14 in both groups of THS patients. The experimental group received intravenous estrogen (25 mg) at a single time point in the ED beside standard of care as per advanced trauma life support guidelines. Patients did not develop any major or minor adverse events and showed favorable clinical outcomes in the experimental group. The levels of T regulatory cells, monocytes, and systemic cytokines significantly reduced and showed a balanced inflammatory response in THS patients who received estrogen. In conclusion, this preliminary study showed that intravenous estrogen therapy is safe and reduced the inflammatory insult due to trauma hemorrhagic shock. It may protect THS patients from sepsis-associated complications. Future clinical trials are required to study the efficacy and mechanistic pathway.

show abstract

“…[50,51] On the other hand, carriage of allele LTa-252 (Lymphotoxin-alpha, formerly known as TNF-b), has been found to be related to an increased production of TNF-a both in vitro [52] and in vivo. [53,54] Kornman and Di Giovine [55] reported an increased TNF-a-308n2 allele frequency in Caucasian patients with chronic periodontitis compared with periodontally healthy subjects. A correlation was also established with the severity of periodontal disease.…”

Section: Introductionmentioning

confidence: 99%

Gene polymorphisms in periodontitis. Overview

Dosseva-Panova

Mlachkova

Popova

2015

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Today, it is known that pathogenic bacteria are the key factors for the initiation of periodontal disease, but the host response and the severity of clinical expression are largely determined by genetic susceptibility and environmental factors. There is evidence that the individual response to the environment and variations in the immune response in periodontitis are associated with genetic factors. Of particular interest are the polymorphisms of interleukin-1 beta (IL-1b), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-a) genes and the factors that determine the production of these cytokines, since they play an important role in the pathophysiology of inflammation and in particular, in periodontal diseases. The identification of a specific IL-1 family genotype which correlates with the severity of periodontitis, demonstrates that it is genetic mechanisms that determine the intensity of immunoinflammatory response in different individuals (and hence, the severity of periodontal disease) due to the presence and persistence of bacterial plaque. This mini-review focuses on the current state of knowledge of genetic polymorphisms in patients with chronic periodontitis. Extensive studies on genetic factors in chronic periodontitis raise hopes for the identification of determinants related to disease susceptibility and improved diagnosis and treatment of chronic periodontitis, as well as prediction of disease progression.

show abstract

Alterations in Leukocyte Function following Surgical Trauma: Differentiation of Distinct Reaction Types and Association with Tumor Necrosis Factor Gene Polymorphisms

Cited by 11 publications

References 24 publications

Functional immune monitoring in severely injured patients—A pilot study

Functional immune monitoring in severely injured patients—A pilot study

Estrogen as a Safe Therapeutic Adjunct in Reducing the Inflammatory Storm in Trauma Hemorrhagic Shock Patients

Gene polymorphisms in periodontitis. Overview

Contact Info

Product

Resources

About