Altered membrane free unsaturated fatty acid composition in human colorectal cancer tissue

Szachowicz-Petelska, Barbara; Sulkowski, Stanisław; Figaszewski, Zbigniew A.

doi:10.1007/s11010-006-9264-x

Cited by 41 publications

(29 citation statements)

References 40 publications

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“…For these reasons, many researchers have focused their attention on possible associations between CRC progression and alterations in lipid levels. Swachowicz-Petelska et al observed that linoleic (LA) and α-linolenic (ALA) acid levels were lower in the cell membranes of colorectal cancer cells, whereas the levels of oleic (OA) and arachidonic acids were increased [12]. Moreover, these results were in accord with other observations of decreased levels of LA and ALA in the plasma of colorectal cancer patients [13,14].…”

Section: Introductionsupporting

confidence: 72%

Altered plasma levels of decanoic acid in colorectal cancer as a new diagnostic biomarker

et al. 2016

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Colorectal cancer (CRC) is one of the most common tumors in developed countries. The five-year survival rate decreases depending on how advanced the CRC is when first diagnosed. Screening has been proven to greatly reduce mortality from colorectal cancer, but an ideal screening tool is far from being established. Here, we aimed to discover and validate early CRC biomarkers by means of an untargeted/targeted metabolomic approach. A preliminary untargeted analysis of plasma lipids performed on a small patient cohort (30 plasma samples) revealed some alterations that occurred in the presence of this tumor. In particular, medium-chain fatty acids with between six and twelve carbon atoms (C6-C12) were found to be the lipid class that showed the most marked changes upon the development of CRC. In order to evaluate the utility of this lipid class as diagnostic CRC biomarkers, a further study based on a wider cohort of patients (117 plasma samples) was performed. Using a targeted approach, these fatty acids were quantified in plasma samples by means of fast gas chromatography coupled to a time-of-flight analyzer. Plasma samples from patients with CRCs at different tumor stages were analyzed and compared to those from healthy subjects, ulcerative colitis patients, high-grade dysplasia adenoma patients, and breast cancer patients in order to test the specificity and sensitivity of these possible biomarkers. Results revealed significant differences among the considered groups in terms of their C6, C8, C10, and C12 fatty acid plasma concentrations. In particular, receiver operating characteristic (ROC) curves obtained for the C10 fatty acid gave an area under the curve of 0.8195 along with a sensitivity of 87.8 % and a specificity of 80 %, strongly suggesting that it could be a valuable early diagnostic biomarker of CRC.

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Section: Introductionsupporting

confidence: 72%

Altered plasma levels of decanoic acid in colorectal cancer as a new diagnostic biomarker

et al. 2016

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“…It is this potential that is the underlying characteristic of the cell that could distinguish between normal and cancerous cells. It was been noted before that cancerous cell have a lower membrane potential, and it has been shown that the cell's membrane changes during cancer transformation, in particular the membrane's fluidity and charge 41-42. Therefore, by altering the cell membrane potential, one could possibly control the fate of the cell.…”

Section: Discussionmentioning

confidence: 99%

Immuno Nanoparticles Integrated Electrical Control of Targeted Cancer Cell Development Using Whole Cell Bioelectronic Device

Hondroulis¹,

Zhang²,

Zhang³

et al. 2014

Theranostics

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Electrical properties of cells determine most of the cellular functions, particularly ones which occur in the cell's membrane. Manipulation of these electrical properties may provide a powerful electrotherapy option for the treatment of cancer as cancerous cells have been shown to be more electronegative than normal proliferating cells. Previously, we used an electrical impedance sensing system (EIS) to explore the responses of cancerous SKOV3 cells and normal HUVEC cells to low intensity (<2 V/cm) AC electric fields, determining that the optimal frequency for SKOV3 proliferation arrest was 200 kHz, without harming the non-cancerous HUVECs. In this study, to determine if these effects are cell type dependant, human breast adenocarcinoma cells (MCF7) were subjected to a range of frequencies (50 kHz-2 MHz) similar to the previously tested SKOV3. For the MCF7, an optimal frequency of 100 kHz was determined using the EIS, indicating a higher sensitivity towards the applied field. Further experiments specifically targeting the two types of cancer cells using HER2 antibody functionalized gold nanoparticles (HER2-AuNPs) were performed to determine if enhanced electric field strength can be induced via the application of nanoparticles, consequently leading to the killing of the cancerous cells without affecting non cancerous HUVECs and MCF10a providing a platform for the development of a non-invasive cancer treatment without any harmful side effects. The EIS was used to monitor the real-time consequences on cellular viability and a noticeable decrease in the growth profile of the MCF7 was observed with the application of the HER2-AuNPs and the electric fields indicating specific inhibitory effects on dividing cells in culture. To further understand the effects of the externally applied field to the cells, an Annexin V/EthD-III assay was performed to determine the cell death mechanism indicating apoptosis. The zeta potential of the SKOV3 and the MCF7 before and after incorporation of the HER2-AuNPs was also obtained indicating a decrease in zeta potential with the incorporation of the nanoparticles. The outcome of this research will improve our fundamental understanding of the behavior of cancer cells and define optimal parameters of electrotherapy for clinical and drug delivery applications.

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“…Accordingly, different biological conditions are associated with differences in membrane phospholipids composition particularly during cancer transformation (Dobrzyńska et al, 2005;Szachowicz-Petelska et al, 2007). For example, most cases of colorectal cancer involve an increase in the concentration of all phospholipid types at the cell membrane, including: phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) ( Table 1).…”

Section: Changes In the Phospholipids Composition Of Human Colorectalmentioning

confidence: 99%

Characterization of the Cell Membrane During Cancer Transformation

Szachowicz-Petelska¹,

Dobrzyńska²,

Sulkowski³

et al. 2012

Colorectal Cancer Biology - From Genes to Tumor

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Altered membrane free unsaturated fatty acid composition in human colorectal cancer tissue

Cited by 41 publications

References 40 publications

Altered plasma levels of decanoic acid in colorectal cancer as a new diagnostic biomarker

Altered plasma levels of decanoic acid in colorectal cancer as a new diagnostic biomarker

Immuno Nanoparticles Integrated Electrical Control of Targeted Cancer Cell Development Using Whole Cell Bioelectronic Device

Characterization of the Cell Membrane During Cancer Transformation

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