Altered physiochemical properties in industrially synthesized ZnO nanoparticles regulate oxidative stress; induce in vivo cytotoxicity in embryonic zebrafish by apoptosis

Verma, Suresh K.; Panda, Pritam Kumar; Jha, Ealisha; Suar, Mrutyunjay; Parashar, S. K. S.

doi:10.1038/s41598-017-14039-y

Cited by 79 publications

(44 citation statements)

References 107 publications

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“…The conferred molecular interaction was in concomitance with the experimental result and indicated towards the reason of increased apoptosis as influential effect on functionality of tp53 both at mRNA and protein level. Similar effect has been observed in reports related to effect of other nanoparticles like ZnO, AgNP on zebrafish however the identity of biomolecules were found to be different in each case [24]. The depicted results indicated towards the influence of AuNP on other proteins functionality taking part in physiology of zebrafish which was confirmed by the pathway analysis as shown in Figure 12.…”

Section: Discussionsupporting

confidence: 85%

“…It can be argued that AuNP and CAuNP affect the hatching due to their duel action. Clogging of the pores of chorion and secondly by affecting the functionality of He1a enzyme due to firm interaction with them [24]. Clear indication of clogging effect was evident by the embryos exposed to higher concentration of AuNP.…”

Section: Discussionmentioning

confidence: 94%

“…In order to determine the mechanism of toxicity possessed by CAuNP and AuNP, uptake analysis was performed along with deduction of intracellular ROS with the help of flow cytometry. Seventy-four hours treated and untreated zebrafish embryos were sacrificed and their single cell suspension was prepared by mild sonication [24,25]. Single cell suspension was stained with 1.25 mg/l H 2 DCFDA dye for 20 min in dark and then washed with 1X phosphate buffer solution for determination of ROS.…”

Section: Nanoparticles Uptake and Intracellular Ros Analysismentioning

confidence: 99%

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Molecular investigation to RNA and protein based interaction inducedin vivobiocompatibility of phytofabricated AuNP with embryonic zebrafish

Verma

Jha

Panda

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Implication of gold nanoparticles in industrial and day-today life products at extensive scale has raised concern about their toxicity to environment and human health. Moreover, quest of new technologies for production of biocompatible nanoparticles increased. This study explores the molecular toxicology of AuNP with enlightenment of their green synthesis using medicinal plant extract as reducing and stabilizing agent. Synthesized CAuNP were characterized for their physiochemical properties by standard techniques like FESEM, TEM, DLS, UV-Vis spectroscopy and FTIR. GCMS analysis revealed the involvement of-OH compounds for CAuNP synthesis. Determined size and zeta potential of CAuNP was found to be 21 ± 08 nm and-24 ± 11 mV with SPR peak at 554 nm. LC50 of CAuNP with zebrafish embryos was 69 ± 12 mg/ml compared to 52 ± 06 mg/ml of AuNP. Gold nanoparticles were found to exhibit concentration dependent morphological abnormalities with acute effect at cellular and molecular level. Experimental and computational analysis depicted the nanotoxicity of gold nanoparticles as a consequence of oxidative stress generation leading to apoptosis due to their influential interaction with Sod1, He1a and tp53 mRNA and proteins. The investigation deciphered the nanotoxicity of gold nanoparticles and suggested the implication of new green methodology for their future productions.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 94%

Section: Nanoparticles Uptake and Intracellular Ros Analysismentioning

confidence: 99%

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Molecular investigation to RNA and protein based interaction inducedin vivobiocompatibility of phytofabricated AuNP with embryonic zebrafish

Verma

Jha

Panda

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…The centre of the simulation box was the His82 residue which is the entrance of the ligand access channel, and the size of the box was set to 20 * 20 * 20 Å. The partial atomic charges of lignin peroxidase and atrazine were calculated with the Gasteiger method 39 , 40 . For every lignin peroxidase conformer, the whole docking process contained 255 docking trajectories.…”

Section: Methodsmentioning

confidence: 99%

Lignin peroxidase ligand access channel dysfunction in the presence of atrazine

Ecker

Fülöp

2018

Sci Rep

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Studies have determined that the white-rot basidiomycete Phanerochaete chrysosporium is capable of biodegrading the atrazine herbicide with its broad-specificity enzymes, but the particular role of biocatalysts is still unclear. In the case of lignin peroxidase, a ligand access channel connected to the active heme cofactor provides access to the active site for potential small-sized substrates. Experimental results show that lignin peroxidase is unable to degrade atrazine, therefore, the primary goal was to determine whether there is any connection between the structural and dynamical properties of the enzyme and its incapability to degrade atrazine. The results of protein-ligand docking and molecular dynamics study correlate with relevant, published NMR and molecular dynamics data, and give the answer to the lack of atrazine degradation by lignin peroxidase which has already been established by numerous authors using experimental methods. Atrazine has no access to heme edge due to the electric charges of the delocalized s-triazine ring. The detected phenomenon suggests that the small size of the ligands only is not a sufficient condition to access the active site. Their physicochemical properties influence the structural behaviour of the channel.

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“…ZnO was selected for this study because it is a well-studied system for crystal growth from solution 32 – 44 . The biological and technological significance of ZnO underline its selection 45 – 48 . Synthesis was carried out by reacting aqueous solutions of 5 mM zinc nitrate ( 1 ) and 50 mM urea ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

A conceptual change in crystallisation mechanisms of oxide materials from solutions in closed systems

Padmanabhan

Collins

Pillai

et al. 2020

Sci Rep

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Atomic and molecular level interactions in solutions dictate the structural and functional attributes of crystals. These features clearly dictate the properties of materials and their applicability in technologies. However, the microscopic phenomena of particle formation—nucleation and growth—in real systems are still not fully understood. Specifically, crystallisation occurring in closed systems are largely unproven. Combining coherent experimental data, we here demonstrate a fundamental nucleation-growth mechanism that occurs in a model zinc oxide system when particles are formed under continuous, rapid heating under closed reaction conditions. Defying all previous reports, we show that the nucleation commences only when the heating is terminated. A prenucleation clusters pathway is observed for nucleation, followed by crystallite assembly-growth. We show that the nucleation-growth processes result from temporal and dynamic activity of constituent ions and gaseous molecules in solution and by the irreversible expulsion of the dissolved gaseous molecules. We suggest that this nucleation process is generic to most closed systems that go through precipitation, and, therefore, important for the crystallisation of a variety of metal oxides, composites and minerals. We anticipate that the work may be a platform for future experimental and theoretical investigation promoting deeper understanding of the nucleation-growth phenomena of a variety of practical systems.

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Altered physiochemical properties in industrially synthesized ZnO nanoparticles regulate oxidative stress; induce in vivo cytotoxicity in embryonic zebrafish by apoptosis

Cited by 79 publications

References 107 publications

Molecular investigation to RNA and protein based interaction inducedin vivobiocompatibility of phytofabricated AuNP with embryonic zebrafish

Molecular investigation to RNA and protein based interaction inducedin vivobiocompatibility of phytofabricated AuNP with embryonic zebrafish

Lignin peroxidase ligand access channel dysfunction in the presence of atrazine

A conceptual change in crystallisation mechanisms of oxide materials from solutions in closed systems

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