Alternaric acid stimulates phosphorylation of His-tagged RiCDPK2, a calcium-dependent protein kinase in potato plants

Hassan, Amjad; Okuta, T.; Kato, Mie; Hatsugai, Noriyuki; Sano, Yumiko; Ishimori, T.; Okazaki, Katsuo; Doullah, M.A.; Shah, Mohibullah

doi:10.4238/2012.may.10.1

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…During plant-pathogen interactions, plants trigger a series of defense mechanisms to protect themselves, which include changes in antioxidant enzymes, increase in PR proteins, and phytoalexin biosynthesis [52]. Calcium-dependent Protein Kinases (CDPKs), involved in the regulation of a myriad of growth and developmental process, signal transduction and pathogenesis, exist widely in eukaryotic cells [53,54]. In this study, the expression of CDPKs was altered considerably, indicating that CDPKs play critical roles in the signaling transduction of defense responses to Lxx infection in sugarcane.…”

Section: Plos Onementioning

confidence: 99%

A transcriptomic analysis of sugarcane response to Leifsonia xyli subsp. xyli infection

Zhu

Yang

et al. 2021

PLoS ONE

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Sugarcane ratoon stunting disease (RSD) caused by Leifsonia xyli subsp. xyli (Lxx) is a common destructive disease that occurs around the world. Lxx is an obligate pathogen of sugarcane, and previous studies have reported some physiological responses of RSD-affected sugarcane. However, the molecular understanding of sugarcane response to Lxx infection remains unclear. In the present study, transcriptomes of healthy and Lxx-infected sugarcane stalks and leaves were studied to gain more insights into the gene activity in sugarcane in response to Lxx infection. RNA-Seq analysis of healthy and diseased plants transcriptomes identified 107,750 unigenes. Analysis of these unigenes showed a large number of differentially expressed genes (DEGs) occurring mostly in leaves of infected plants. Sugarcane responds to Lxx infection mainly via alteration of metabolic pathways such as photosynthesis, phytohormone biosynthesis, phytohormone action-mediated regulation, and plant-pathogen interactions. It was also found that cell wall defense pathways and protein phosphorylation/dephosphorylation pathways may play important roles in Lxx pathogeneis. In Lxx-infected plants, significant inhibition in photosynthetic processes through large number of differentially expressed genes involved in energy capture, energy metabolism and chloroplast structure. Also, Lxx infection caused down-regulation of gibberellin response through an increased activity of DELLA and down-regulation of GID1 proteins. This alteration in gibberellic acid response combined with the inhibition of photosynthetic processes may account for the majority of growth retardation occurring in RSD-affected plants. A number of genes associated with plant-pathogen interactions were also differentially expressed in Lxx-infected plants. These include those involved in secondary metabolite biosynthesis, protein phosphorylation/dephosphorylation, cell wall biosynthesis, and phagosomes, implicating an active defense response to Lxx infection. Considering the fact that RSD occurs worldwide and a significant cause of sugarcane productivity, a better understanding of Lxx resistance-related processes may help develop tools and technologies for producing RSD-resistant sugarcane varieties through conventional and/or molecular breeding.

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Section: Plos Onementioning

confidence: 99%

A transcriptomic analysis of sugarcane response to Leifsonia xyli subsp. xyli infection

Zhu

Yang

et al. 2021

PLoS ONE

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“…[16,17] The same year, Ichihara and co-workers reported that 2 exhibited phytotoxic activity against tomato seedlings. [18] However, the MoA remains unknown [19,20] and limited SAR has been reported: the only data relates to an observed loss of activity when the C10 methylene or C15 hydroxyl motifs are removed (Fig. 1B).…”

Section: Introductionmentioning

confidence: 99%

Total synthesis and structure–activity relationship of alternaric acid delivers an herbicide vector

Israel

Comas-Barceló²,

Slawin³

et al. 2022

Nat. Synth

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Global food security is one of the foremost challenges of our time requiring a multifaceted solution. Crop protection strategies are an essential part of this response; however, there is increasing resistance to known modes of action (MoA). Since its discovery in 1949, the natural product Alternaric acid has been proposed as a starting point for herbicide development. However, this target is undeveloped owing to its poor synthetic accessibility and a lack of knowledge of the associated pharmacology. Here we report the discovery of herbicidal compounds from Alternaric acid that operate via a potentially unknown MoA. Development of a total synthesis enabled structure-activity relationship (SAR) profiling of compound libraries, which, combined with phenotypic screening and molecular modelling data, identified small molecule lead compounds with enhanced and broader spectrum herbicidal activity than Alternaric acid.

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“…Evidence suggests that for each stress condition, specific temporal, spatial, and concentration variations in cytosolic free calcium (Ca 2+ ) are generated (Webb et al, 1996). These calcium signatures can be decoded and modulated by calcium-binding proteins, which bind calcium through their EF-hands and relay the signal by activating kinases, transcription factor, or directly acting at promoters of effector genes (DeFalco et al, 2010;Hassan et al, 2012). One group of plant-specific calcium-binding proteins comprises the calcineurin B-like (CBL) proteins (Shi et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a protein-protein interaction network of the CBL-interacting protein kinase 8 from sugarcane

Farani¹,

Gentile²,

Tavares³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Plants are sessile organisms and have evolved to tolerate a constantly changing environment. After the onset of different stress conditions, calcineurin B-like (CBL) proteins can sense calcium signals and activate CBL-interacting protein kinase (CIPK) proteins, which can phosphorylate downstream proteins to reestablish plant homeostasis. Previous studies in the bioenergy crop sugarcane showed that the ScCIPK8 gene is induced by drought stress and is also related to sucrose content. Here, we have characterized the protein-protein interactions of ScCIPK8 with six CBL proteins (ScCBL1, ScCBL2, ScCBL3, ScCBL6, ScCBL9, and ScCBL10). Yeast two-hybrid assays showed that ScCIPK8 interacts with ScCBL1, ScCBL3, and ScCBL6. Bimolecular fluorescence complementation assays confirmed in planta the interactions that were observed in yeast cells. These findings give insights on the regulatory networks related to sugar accumulation and drought stress responses in sugarcane.

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Alternaric acid stimulates phosphorylation of His-tagged RiCDPK2, a calcium-dependent protein kinase in potato plants

Cited by 7 publications

References 22 publications

A transcriptomic analysis of sugarcane response to Leifsonia xyli subsp. xyli infection

A transcriptomic analysis of sugarcane response to Leifsonia xyli subsp. xyli infection

Total synthesis and structure–activity relationship of alternaric acid delivers an herbicide vector

Characterization of a protein-protein interaction network of the CBL-interacting protein kinase 8 from sugarcane

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