Characterization of a protein-protein interaction network of the CBL-interacting protein kinase 8 from sugarcane

Farani, T. F.; Gentile, Alessandra; Tavares, Rafael Garcia; Ribeiro, Cauê; Menossi, Marcelo

doi:10.4238/2015.january.26.1

Cited by 10 publications

(8 citation statements)

References 32 publications

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“…Previous studies have indicated that the CIPK gene may have a link with the sucrose content of plants. For example, ScCIPK8 , a sugarcane CIPK gene, had a negative correlation with sucrose content of leaves [34,35]. Light-interruption stress, which inhibits the synthesis of sucrose, reduces the expression of AtCIPK14 expression; after feeding sucrose, however, the expression of AtCIPK14 goes up [36].…”

Section: Discussionmentioning

confidence: 99%

Circadian Regulation of Alternative Splicing of Drought-Associated CIPK Genes in Dendrobium catenatum (Orchidaceae)

Xiao

Zou

Zheng

et al. 2019

IJMS

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Dendrobium catenatum, an epiphytic and lithophytic species, suffers frequently from perennial shortage of water in the wild. The molecular mechanisms of this orchid’s tolerance to abiotic stress, especially drought, remain largely unknown. It is well-known that CBL-interacting protein kinase (CIPKs) proteins play important roles in plant developmental processes, signal transduction, and responses to abiotic stress. To study the CIPKs’ functions for D. catenatum, we first identified 24 CIPK genes from it. We divided them into three subgroups, with varying intron numbers and protein motifs, based on phylogeny analysis. Expression patterns of CIPK family genes in different tissues and in response to either drought or cold stresses suggested DcaCIPK11 may be associated with signal transduction and energy metabolism. DcaCIPK9, -14, and -16 are predicted to play critical roles during drought treatment specifically. Furthermore, transcript expression abundances of DcaCIPK16 showed polar opposites during day and night. Whether under drought treatment or not, DcaCIPK16 tended to emphatically express transcript1 during the day and transcript3 at night. This implied that expression of the transcripts might be regulated by circadian rhythm. qRT-PCR analysis also indicated that DcaCIPK3, -8, and -20 were strongly influenced by circadian rhythmicity. In contrast with previous studies, for the first time to our knowledge, our study revealed that the major CIPK gene transcript expressed was not always the same and was affected by the biological clock, providing a different perspective on alternative splicing preference.

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Section: Discussionmentioning

confidence: 99%

Circadian Regulation of Alternative Splicing of Drought-Associated CIPK Genes in Dendrobium catenatum (Orchidaceae)

Xiao

Zou

Zheng

et al. 2019

IJMS

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“…CIPK14 has been shown to play a role in conferring drought tolerance in sugarcane [ 37 ]. Farani et al [ 38 ] found that CIPK8 is not only induced by drought stress but also related to sucrose content.…”

Section: Introductionmentioning

confidence: 99%

New insights into the evolution and functional divergence of the CIPK gene family in Saccharum

Ren

Wang

et al. 2020

BMC Genomics

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Background Calcineurin B-like protein (CBL)-interacting protein kinases (CIPKs) are the primary components of calcium sensors, and play crucial roles in plant developmental processes, hormone signaling transduction, and in the response to exogenous stresses. Results In this study, 48 CIPK genes (SsCIPKs) were identified from the genome of Saccharum spontaneum. Phylogenetic reconstruction suggested that the SsCIPK gene family may have undergone six gene duplication events from the last common ancestor (LCA) of SsCIPKs. Whole-genome duplications (WGDs) served as the driving force for the amplification of SsCIPKs. The Nonsynonymous to synonymous substitution ratio (Ka/Ks) analysis showed that the duplicated genes were possibly under strong purifying selection pressure. The divergence time of these duplicated genes had an average duplication time of approximately 35.66 Mya, suggesting that these duplication events occurred after the divergence of the monocots and eudicots (165 Mya). The evolution of gene structure analysis showed that the SsCIPK family genes may involve intron losses. Ten ScCIPK genes were amplified from sugarcane (Saccharum spp. hybrids). The results of real-time quantitative polymerase chain reaction (qRT-PCR) demonstrated that these ten ScCIPK genes had different expression patterns under abscisic acid (ABA), polyethylene glycol (PEG), and sodium chloride (NaCl) stresses. Prokaryotic expression implied that the recombinant proteins of ScCIPK3, − 15 and − 17 could only slightly enhance growth under salinity stress conditions, but the ScCIPK21 did not. Transient N. benthamiana plants overexpressing ScCIPKs demonstrated that the ScCIPK genes were involved in responding to external stressors through the ethylene synthesis pathway as well as to bacterial infections. Conclusions In generally, a comprehensive genome-wide analysis of evolutionary relationship, gene structure, motif composition, and gene duplications of SsCIPK family genes were performed in S. spontaneum. The functional study of expression patterns in sugarcane and allogenic expressions in E. coli and N. benthamiana showed that ScCIPKs played various roles in response to different stresses. Thus, these results improve our understanding of the evolution of the CIPK gene family in sugarcane as well as provide a basis for in-depth functional studies of CIPK genes in sugarcane.

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“…is an economically attractive polyploid C4 grass that is used not only to produce approximately 60% of the world's sugar but also to produce ethanol, a low-carbon-emission fuel 25 . To date, there have been few reports on CBL genes in sugarcane [26][27][28] . Zhang (2013) cloned five CBL genes (GenBank accession Nos.…”

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confidence: 99%

“…Using real-time quantitative polymerase chain reaction (qRT-PCR) analyses, found that SsCBL1 and SsCBL6 play important regulatory roles in response to a variety of stresses (low potassium, drought, and salt) 26 . Yeast two-hybrid assays showed that ScCIPK8 interacts with ScCBL1, ScCBL3, and ScCBL6 27 . In the past 15 years, ROC22 is the most widely cultivated sugarcane in China due to its high yield and high sugar and good ratoon properties.…”

mentioning

confidence: 99%

Sugarcane calcineurin B-like (CBL) genes play important but versatile roles in regulation of responses to biotic and abiotic stresses

Huang

Ling

et al. 2020

Sci Rep

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free calcium ions are common second messengers in plant cells. the calcineurin B-like protein (cBL) is a special calcium sensor that plays an important role in plant growth and stress response. in this study, we obtained three CBL genes (GenBank accession nos. KX013374, KX013375, and KX013376) from sugarcane variety ROC22. The open reading frames of ScCBL genes ranged from 642 to 678 base pairs in length and encoded polypeptides from 213 to 225 amino acids in length. ScCBL2-1, ScCBL3-1, and ScCBL4 were all located in the plasma membrane and cytoplasm. ScCBL2-1 and ScCBL3-1 expression was up-regulated by treatment with salicylic acid (SA), methyl jasmonate (MeJA), hydrogen peroxide (H 2 o 2 ), polyethylene glycol (peG), sodium chloride (nacl), or copper chloride (cucl 2 ). ScCBL4 expression was down-regulated in response to all of these stresses (abscisic acid (ABA), SA, MeJA, and nacl) except for H 2 o 2 , calcium chloride (cacl 2 ), peG, and cucl 2 . expression in Escherichia coli BL21 cells showed that SccBLs can enhance tolerance to nacl or copper stress. overexpression of ScCBLs in Nicotiana benthamiana leaves promoted their resistance to infection with the tobacco pathogen Ralstonia solanacearum. the results from the present study facilitate further research regarding ScCBL genes, and in particular, their roles in the response to various stresses in sugarcane.Calcium (Ca 2+ ) is an important inorganic nutritive element and a ubiquitous second messenger 1 . Ca 2+ not only plays a vital role in maintaining the stability of the cell wall, cell membrane, and membrane binding proteins, but also is widely involved in the regulation and control of plant growth and development, as well as response to external environmental stimuli 2 . In plants, intracellular Ca 2+ sensors accurately recognize specific Ca 2+ signatures that are generated in response to different external stimuli 3 . Under adverse conditions, cell signal transduction receptors on the plant cell membrane recognize the stimulus signal, and then activate the Ca 2+ channel protein through phosphorylation, thereby leading to an instantaneous increased in the Ca 2+ concentration in the cytoplasm, which produces the "Ca 2+ signal" 4 . Ca 2+ sensors in plants detect this stress-induced Ca 2+ signal and deliver it to downstream effectors, activating a signal cascade reaction that regulates resistance and tolerance 5 . Ca 2+ sensors can be divided into two main types according to their structural features. One type is the sensor responders, including calcium-dependent protein kinases and calmodulins, which have all the functions of Ca 2+ sensor relay proteins, as well as the kinase activity 6 . The other type is the sensor relays, including calmodulin-like proteins and CBL, which do not have kinase activity. Sensor relays can specifically target downstream proteins to transfer the perceived Ca 2+ signals in response to various environmental stimuli 6 .CBL, a Ca 2+ sensor relay protein that is expressed in a wide range of plants, can interact with a family of s...

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Characterization of a protein-protein interaction network of the CBL-interacting protein kinase 8 from sugarcane

Cited by 10 publications

References 32 publications

Circadian Regulation of Alternative Splicing of Drought-Associated CIPK Genes in Dendrobium catenatum (Orchidaceae)

Circadian Regulation of Alternative Splicing of Drought-Associated CIPK Genes in Dendrobium catenatum (Orchidaceae)

New insights into the evolution and functional divergence of the CIPK gene family in Saccharum

Sugarcane calcineurin B-like (CBL) genes play important but versatile roles in regulation of responses to biotic and abiotic stresses

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