Alternative Method of Rapid Drying Vascular Specimens for Scanning Electron Microscopy

Slížová, D; Krs, Otakar; Pospísilová, B

doi:10.1177/152660280301000218

Cited by 11 publications

(7 citation statements)

References 8 publications

(8 reference statements)

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“…These two methods avoid producing the surface-tension and capillary effects which can adversely distort microscopic structure ( Favret 2009 ). Alternative chemical approaches include using solvents with low surface tension such as hexamethyldisilazane (HMDS) ( Nation 1983 , Slížová et al 2003 , Buravkov et al 2011 ) or Acetone ( Van Noort 1996 ). Both methods result in greatly reduced deformation of the soft structures in less sclerotized insects.…”

Section: Discussionmentioning

confidence: 99%

Preservation of iridescent colours in Phorinia Robineau-Desvoidy, 1830 (Diptera: Tachinidae)

Braet¹,

Downes²,

Simonis³

2016

BDJ

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BackgroundIridescent blue-green colours are exhibited by various organisms including several taxa in the Tachinidae (Diptera) with notable examples within the Afrotropical members of the genus Phorinia Robineau-Desvoidy, 1830. The vivid colouration observed in life quickly fades to a dull golden-yellow when a specimen is dried. Although well known, no published explanation has been given for this phenomenon.New informationWe illustrate the mechanism associated with this colour change. We also test and propose technical alternatives to retain the living colours in dried specimens.

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Section: Discussionmentioning

confidence: 99%

Preservation of iridescent colours in Phorinia Robineau-Desvoidy, 1830 (Diptera: Tachinidae)

Braet¹,

Downes²,

Simonis³

2016

BDJ

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“…The scaffolds were washed with distilled water, dehydrated with hexamethyldisilazane, and analyzed by SEM after being coated with gold. [ 33 ]…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of surface aminolysis and hydrolysis on improving fibroblast cell colonization within poly(L‐lactide) scaffolds

Bakry

2020

J of Applied Polymer Sci

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This work presents a method for overcoming the low internal cell colonization within the tissue engineering scaffolds. 3D scaffolds were fabricated by the compression molding/salt leaching technique from mixtures of aminolyzed and hydrolyzed poly(L-lactide) single crystals (PLLAsc) with poly(D,L-lactide) (PDLLA) microparticles. Modified PLLAsc were used to guarantee the surface hydrophilicity, while PDLLA microparticles were used to reinforce the scaffolds. Mechanical properties, morphology, water uptake and fibroblast cell response of the scaffolds were investigated in comparison with scaffolds from only pristine, hydrolyzed, or aminolyzed PLLAsc. Results showed that the scaffolds exhibited proper morphology and hydrophilicity with an enhanced strength. Water uptake by scaffolds from modified PLLAsc was 1.9 times that from pristine PLLAsc. The scaffolds' compressive moduli increased 2.5 times by using of PDLLA (30 wt%). After cell seeding for a week, the number of cells founded on aminolyzed/hydrolyzed PLLAsc scaffolds were 4.28, 2.14, and 1.36 times that founded for pristine, aminolyzed and hydrolyzed PLLAsc scaffolds, respectively. SEM studies showed that fibroblasts migrated and spread throughout the aminolyzed/hydrolyzed PLLAsc scaffolds better than other scaffolds. Hence, combining both aminolyzed and hydrolyzed PLLAsc with PDLLA could result in a mechanically stable scaffold with an enhanced cell colonization.

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“…Cells were fixed after 7 days of culture by 2.5 vol % glutaraldehyde solution in 0.1 mol L −1 cacodylate buffer (pH 7.2) overnight. The scaffolds were washed with distilled water, dehydrated with hexamethyldisilazane, and finally coated with gold for SEM analysis …”

Section: Methodsmentioning

confidence: 99%

“…The scaffolds were washed with distilled water, dehydrated with hexamethyldisilazane, and finally coated with gold for SEM analysis. 25…”

Section: Uniformity Of Heparin Throughout the Scaffoldmentioning

confidence: 99%

Synergistic effects of surface grafting with heparin and addition of poly(d,l‐lactide) microparticles on properties of poly(l‐lactide) single crystals scaffolds

Bakry

2019

J of Applied Polymer Sci

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Mixtures of heparin grafted poly(l‐lactide) single crystals (Hep‐PLLAsc) and poly(d,l‐lactide) (PDLLA) microparticles are used for assembling hydrophilic and cytocompatible three‐dimensional tissue engineering scaffolds simply by compression molding/salt leaching technique. PLLA is grafted with heparin to promote its surface cytocompatability, while PDLLA is added to reinforce the scaffolds. PLLAsc are aminolyzed with tetraethylenepentamine (13‐atom spacer) to generate sterically accessible amino groups at the surface allowing the covalent attachment of heparin by aqueous carbodiimide chemistry. Morphological and compressive strength studies manifest the integrity and compactness of scaffolds. Toluidine blue assay assures the consistency of heparin distribution throughout the scaffolds, which is in contrary to the conventional grafting reactions by immersing the scaffold in heparin solution. The scaffolds applicability is examined by fibroblast cells seeding experiments. Contrary to pristine scaffolds, Hep‐PLLAsc scaffolds display a hydrophilic and cytocompatible interface for cell adhesion, spreading, growth, and migration. Therefore, combining presurface grafted PLLAsc with PDLLA microparticles could offer durable scaffolds exhibiting bioactive interface and controllable spatial distribution of the grafted biomacromolecules. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47797.

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Alternative Method of Rapid Drying Vascular Specimens for Scanning Electron Microscopy

Cited by 11 publications

References 8 publications

Preservation of iridescent colours in Phorinia Robineau-Desvoidy, 1830 (Diptera: Tachinidae)

Preservation of iridescent colours in Phorinia Robineau-Desvoidy, 1830 (Diptera: Tachinidae)

Synergistic effects of surface aminolysis and hydrolysis on improving fibroblast cell colonization within poly(L‐lactide) scaffolds

Synergistic effects of surface grafting with heparin and addition of poly(d,l‐lactide) microparticles on properties of poly(l‐lactide) single crystals scaffolds

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