Aluminum exposure alters behavioral parameters and increases acetylcholinesterase activity in zebrafish (Danio rerio) brain

Senger, Mário Roberto; Seibt, Kelly Juliana; Ghisleni, Gabriele; Dias, Renato Dutra; Bogo, Maurı́cio Reis; Bonan, Carla Denise

doi:10.1007/s10565-011-9181-y

Cited by 66 publications

(38 citation statements)

References 38 publications

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“…The decrease of locomotor activity after a short treatment with aluminum have also been observed in vertebrates (Abdel‐Aal et al ., ; Senger et al ., ) and seems to be correlated with the brain damage, especially in the cholinergic system. The analysis of locomotor activity is a common method to examine aging (Rakshit et al ., ) and neurotoxic effects of various substances including aluminum (Senger et al ., ). In this study we observed changes in behavior but did not test biochemical changes in the fruit fly brain.…”

Section: Discussionmentioning

confidence: 89%

Drosophila melanogaster as a model system of aluminum toxicity and aging

et al. 2013

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The aim of this study was to investigate the toxic effects of aluminum (Al) on the model organism-Drosophila melanogaster. The study is especially concerned with the effects of aluminum on the fruit fly's development, life span, and circadian rhythm in rest and activity. Flies were exposed to aluminum in concentrations from 40 to 280 mg/kg in rearing media or the flies were raised on control medium. Moreover, the life span of insects exposed to aluminum containing 40, 120, or 240 mg/kg of Al in the medium, only during their larval development, during the whole life cycle and only in their adult life was tested. To check if aluminum and aging cause changes in D. melanogaster behavior, the locomotor activity of flies at different ages was recorded. Results showed that aluminum is toxic in concentrations above 160 mg/kg in the rearing medium. Depending on Al concentration and time of exposure, the life span of the flies was shortened. At intermediate concentrations (120 mg/kg), however, Al had a stimulating effect on males increasing their life span and level of locomotor activity. At higher concentration the aluminum exposure increased or decreased the level of locomotor activity of D. melanogaster depending on age of flies. In addition, in the oldest insects reared on aluminum supplemented media and in mid-aged flies reared on the highest concentration of Al the daily rhythm of activity was disrupted.

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Section: Discussionmentioning

confidence: 89%

Drosophila melanogaster as a model system of aluminum toxicity and aging

et al. 2013

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“…Because diazepam exerts its effects by binding to neuronal GABA receptors, there is also the potential that chlorpyrifos may modify GABAergic neurons, or GABA levels. Overall, the observed behavioral defects following chlorpyrifos exposure are concerning, especially since long-term behavioral changes induced by fetal exposure to stress, drugs, and environmental toxicants, including chlorpyrifos, have been documented in other species, including humans (Beer et al, 2004; Berkowitz et al, 2004; Dufour-Rainfray et al, 2011; Lazinski et al, 2008; Senger et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Richendrfer¹,

Pelkowski²,

Colwill³

et al. 2012

Neurotoxicology and Teratology

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Neurobehavioral disorders such as anxiety, autism, and attention deficit hyperactivity disorders are typically influenced by genetic and environmental factors. Although several genetic risk factors have been identified in recent years, little is known about the environmental factors that either cause neurobehavioral disorders or contribute to their progression in genetically predisposed individuals. One environmental factor that has raised concerns is chlorpyrifos, an organophosphate pesticide that is widely used in agriculture and is found ubiquitously in the environment. In the present study, we examined the effects of sub-chronic chlorpyrifos exposure on anxiety-related behavior during development using zebrafish larvae. We found that sub-chronic exposure to 0.01 or 0.1 μM chlorpyrifos during development induces specific behavioral defects in 7-day-old zebrafish larvae. The larvae displayed decreases in swim speed and thigmotaxis, yet no changes in avoidance behavior were seen. Exposure to 0.001 μM chlorpyrifos did not affect swimming, thigmotaxis, or avoidance behavior and exposure to 1 μM chlorpyrifos induced behavioral defects, but also induced defects in larval morphology. Since thigmotaxis, a preference for the edge, is an anxiety-related behavior in zebrafish larvae, we propose that sub-chronic chlorpyrifos exposure interferes with the development of anxiety-related behaviors. The results of this study provide a good starting point for examination of the molecular, cellular, developmental, and neural mechanisms that are affected by environmentally relevant concentrations of organophosphate pesticides. A more detailed understanding of these mechanisms is important for the development of predictive models and refined health policies to prevent toxicant-induced neurobehavioral disorders.

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“…It is well known that apoptosis underlies the neurotoxic effects of various compounds. Moreover, zebrafish brain AChE activation has also been demonstrated as a consequence of exposure to known neurotoxic compounds, including aluminum [20] and ethanol [21] and to the cyanobacterial toxin microcystin-LR [50]. …”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of brain AChE activity of aquatic species by toxic substances such as methanol [16], heavy metals mercury, and lead [17], neurotoxins [18], and organochlorine pesticide endosulfan [19] also has been well established. On the other hand, AChE activation has also been demonstrated as a consequence of exposure to neurotoxic compounds such as aluminum [20] and ethanol [21]. …”

Section: Introductionmentioning

confidence: 99%

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

Forno

Kist

Azevedo

et al. 2013

BioMed Research International

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Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested (7.5, 15, and 30 mg/kg) altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano/microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg/kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation (TBARS assays) in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control (RT-qPCR analysis) nor to the direct action of nonphotoexcited C60 nano/microparticles on the protein (in vitro results) but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure.

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Aluminum exposure alters behavioral parameters and increases acetylcholinesterase activity in zebrafish (Danio rerio) brain

Cited by 66 publications

References 38 publications

Drosophila melanogaster as a model system of aluminum toxicity and aging

Drosophila melanogaster as a model system of aluminum toxicity and aging

Developmental sub-chronic exposure to chlorpyrifos reduces anxiety-related behavior in zebrafish larvae

Intraperitoneal Exposure to Nano/Microparticles of Fullerene (C60) Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish (Danio rerio) Brain

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