Alveolar Bone Resorption Following Coronal Versus Apical Approach in a Mucoperiosteal Flap Surgery Procedure in the Rat Mandible

Binderman, I.; Adut, M.; Zohar, Ron; Bahar, Hila; Faibish, Dan; Yaffe, Avinoam

doi:10.1902/jop.2001.72.10.1348

Cited by 39 publications

(50 citation statements)

References 18 publications

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“…A later study by Binderman et al 80 showed that local remodeling of bone is greater when the flap is from a coronal approach rather than from an apical approach.…”

Section: Periosteal Flaps and The Rapmentioning

confidence: 98%

Elevation of a full-thickness mucoperiosteal flap alone accelerates orthodontic tooth movement

Owen¹,

Campbell

Feng

et al. 2017

American Journal of Orthodontics and Dentofacial Orthopedics

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“…A later study by Binderman et al 80 showed that local remodeling of bone is greater when the flap is from a coronal approach rather than from an apical approach.…”

Section: Periosteal Flaps and The Rapmentioning

confidence: 98%

Elevation of a full-thickness mucoperiosteal flap alone accelerates orthodontic tooth movement

Owen¹,

Campbell

Feng

et al. 2017

American Journal of Orthodontics and Dentofacial Orthopedics

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“…It seems that the separation of the gingiva from the tooth and disruption of the dento‐gingival fibers rather than separation of the mucoperiosteum from the alveolar bone is the major trigger for alveolar bone loss 4 . Alveolar bone loss commences only when vertical sectioning of the collagen bundles of the gingiva by a coronal approach (COR) is performed in contrast to an apical surgical approach (AP) 5 . It was also noted that discarding the sectioned marginal gingiva, including the cells, prevented alveolar bone resorption 2,6 .…”

mentioning

confidence: 99%

Topical Application of Drugs Influencing Cytoskeleton and Cell Contractility Affects Alveolar Bone Loss in Rats

Yaffe

Bahar

Binderman

2006

Journal of Periodontology

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Background: Several studies have shown that sectioning bundles of collagen fibers in the marginal gingiva during surgical procedures in animals is a distinct stimulus for alveolar bone resorption. Normally, gingival and periodontal fibroblasts, which reside on these collagen fibers, create physiological traction forces generated by the cytoskeleton. By splitting the fibers, traction forces are released, inducing changes in the cytoskeleton and cell shape. In this study, four drugs were selected, including cytochalasin D, EDTA, sodium orthovanadate, and H‐7, all influencing the cytoskeleton‐integrin‐extracellular matrix (ECM) pathway, for their ability to reduce alveolar bone loss by local application. Methods: The drugs were applied locally only once at the site of mucoperiosteal flap surgery in a rat model. Cytochalasin D (1 μl/μl), EDTA (0.24 mg/μl), sodium orthovanadate (0.02 mg/μl), and H‐7 (0.10 μl/ μl), each separately, were carried by a protective paste and placed immediately after elevating the flap. The analysis of alveolar bone loss was performed 3 weeks after surgery by scanning the microradiographic films of the mandible cross‐sections. The percentages of cross sections with no, moderate, or severe bone loss in treated in comparison to non‐treated rats are presented. Results: EDTA, sodium orthovanadate, and H‐7 were significantly effective in reducing alveolar bone loss. They were effective in reducing the amount of severe bone loss by 53%, 20%, and 58% while increasing the number of sections with no bone loss by 25%, 23%, and 35%, respectively. Cytochalasin D reduced alveolar bone loss insignificantly. Conclusion: EDTA, sodium orthovanadate, and H‐7 are effective in reducing alveolar bone loss in rats following mucoperiosteum surgery.

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“…Three weeks later, a significant bone loss on the periodontal aspect of alveolar bone that extends toward bone crest was recorded [4,18]. In the present study, human gingival fibroblasts (HGF) that were seeded on collagen coated culture dishes normally developed traction forces between the HGF cells and collagen [27][28][29].…”

Section: Discussionmentioning

confidence: 70%

“…Externally applied forces are transmitted by integrins or cadherins into and throughout the cell toward the nuclear membrane, by adhesive proteins that link to cytoskeleton structures [2]. Our previous studies [3][4][5] and others [6,7] have shown that surgical detachment of dento-gingival fibers from root surfaces is a major trigger for alveolar bone resorption, in a rat model because a sudden reduction of physiological strains of adherent fibroblasts ensued [8]. We found that a rapid release of ATP from the cells to the extracellular environment and up-regulated expression of ATP cell membrane receptor P2X4 is main trigger for alveolar bone resorption [9].…”

Section: Introductionmentioning

confidence: 98%

Strain Reduction of Human Gingival Fibroblasts Induces the ATP Pathway

Weinberg¹

2015

J Interdiscipl Med Dent Sci

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Studies in rats demonstrated that surgical detachment of marginal gingiva from root surfaces induced alveolar bone resorption via activation of ATP receptor P2X4. Our aim was to study the effects of strain release of human gingival fibroblasts by detachment of collagen coat from culture dishes. Changes in cell shape, extracellular ATP, cell calcium and genes relevant to trigger alveolar bone resorption were measured. HGF cells from human marginal gingiva explants were seeded on collagen coated culture dishes. In the test group, the collagen coat was detached from culture dish 4-5 days after seeding. Quantification of extracellular ATP was measured by Bioluminescent Assay Kit and changes of intracellular calcium by FLU-AM fluorescence. Real-time PCR was used to examine expression of purinergic receptor P2X4 and P2X7, RANK-L and STC1. Cell length of HGFs grown on plastic surface and on collagen was similar. Reduction in cell length of 45% was measured after detachment of collagen coat. Extracellular ATP raised 10 folds 1 minute after detachment of collagen coat, then declined and returned to control levels 60 minutes later. Ionomycin increased extracellular ATP, while, pretreatment by EGTA, or BAPTA-AM, 30 minutes prior to Ionomycin, reduced significantly extracellular ATP. Reciprocally, addition of ATP increased influx of calcium into HGFs. At the molecular level elevated expression of STC-1, RANK-L and P2X7 was recorded by RT -PCR in detached HGFs, while expression of P2X4 was unchanged. Expression of STC-1, a modulator of cellular calcium and phosphate, P2X7 a calcium ionic channel purinergic receptor and RANK-L a powerful regulator of osteoclastic activity, all were up-regulated significantly. Conclusion:We propose that drop of strain of HGF cells and change in their shape stimulated release of cellular ATP which is signaling through Pi/Ca modulators the molecular activation of osteoclasts.

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Alveolar Bone Resorption Following Coronal Versus Apical Approach in a Mucoperiosteal Flap Surgery Procedure in the Rat Mandible

Abstract: The results of this study show that periodontal surgery by a coronal surgical approach stimulates a burst of remodeling of the alveolar bone in a very specific pattern.

Cited by 39 publications

References 18 publications

Elevation of a full-thickness mucoperiosteal flap alone accelerates orthodontic tooth movement

Elevation of a full-thickness mucoperiosteal flap alone accelerates orthodontic tooth movement

Topical Application of Drugs Influencing Cytoskeleton and Cell Contractility Affects Alveolar Bone Loss in Rats

Strain Reduction of Human Gingival Fibroblasts Induces the ATP Pathway

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