Amelioration of beryllium induced alterations in hepatorenal biochemistry and ultramorphology by co-administration of tiferron and adjuvants

Abstract

Influence of adjuvants i.e., alpha-tocopherol (25 mg/kg, p.o.) and piperine (10 mg/kg, p.o.) on therapeutic potential of chelator tiferron (300 mg/kg, i.p.) was evaluated to encounter toxicogenic events of beryllium exposure. Albino rats were exposed to beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by treatment of aforesaid therapeutic agents for 5 consecutive days. Results were considered to be significant at p < or =0.01 and p < or =0.05. Exposure to beryllium increased its concentration in liver, kidney and serum causing significant alterations in the activity of CYP-450 2E1 system, microsomal lipid peroxidation and protein; alkaline phosphtase, lactate dehydrogenase, gamma-glutamyl transpeptidase, bilirubin, creatinine and urea in serum; activity of acid phosphatase, alkaline phosphatase, adenosine triphosphatase, glucose-6-phosphatase and succinic dehydrogenase in liver and kidney. Beryllium exposure also induced severe alterations in histopathology and ultramorphology of liver and kidney proving its toxic consequences at cellular level. Tiferron along with adjuvants dramatically reversed alterations of all variables more towards control rather than individual treatment. Study concluded that tiferron in combination with alpha-tocopherol and piperine respectively was beneficial in diluting beryllium induced systemic toxicity; however, combination of tiferron and piperine presented more pronounced therapeutic potential.

The Vignette for V14 N3 Issue

“…Present study [9] showed that tiferron in combination of a tocopherol or piperine was effective in reducing the systemic toxicity of beryllium nitrate. Effects of adjuvants, a tocopherol (2 mg/kg, P.O.)…”

Co-administration of adjuvants along withMoringa oleiferaattenuates beryllium-induced oxidative stress and histopathological alterations in rats

“…Beryllium administration decreased enzymatic activity possibly due to binding of beryllium ions to its phosphate group (Aldridge & Thomas, 1966). Depleted enzymatic activity also directly reflects the degradation of SER membrane, which was also evident by ultra morphology of the liver and kidney (Nirala et al, 2007). Treatment with aqueous doses of M. oleifera helps in maintaining integrity of cellular membrane of hepatocytes as shown in histological sections and restored activity of glucose-6-phosphatase.…”

“…Beryllium nitrate (35% w/v; Sigma-Aldrich, St. Louis, MO) was diluted in triple distilled water making up doses of 1 mg/ 2 ml/kg and administered intraperitoneally (Nirala et al, 2007). The doses of M. oleifera root extract (150 mg/kg/5 ml) were prepared in 1% gum acacia, and the doses of piperine (2.5 mg/kg/3 ml) and curcumin (5 mg/kg/3 ml) were prepared in olive oil and administered orally with the help of an intragastric rubber catheter.…”

“…), and other Piper species (Piperaceae). It has bioavailability enhancing activity for some drugs (Nirala et al, 2007(Nirala et al, , 2008. Piperine is known to exhibit a variety of biological activities including antiinflammatory (Ying et al, 2013), antioxidant (Jain & Mishra, 2011), antithyroid (Vijayakumar & Nalini, 2006), antimutagenic (Srinivasan, 2007), antitumor (Li et al, 2011), antidepressant (Mao et al, 2011), and hepatoprotective activity (Sahu et al, 2012).…”

Study on the effects of nitrilotriproprionic acid and 4,5-dihydroxy-1,3-benzene disulphonate on the fractionation of beryllium in human serum using graphite furnace atomic absorption spectrometry

“…Tiron is a hydrophilic chelator and was selected because of its reported efficiency in mobilising beryllium [15,16], restoring the altered biochemical parameters [17] and improving the altered hepatorenal biochemistry and ultramorphology in different rat tissues and organs [18]. …”