Amelioration of CCl4-induced liver injury in rats by selenizing Astragalus polysaccharides: Role of proinflammatory cytokines, oxidative stress and hepatic stellate cells

Hamid, Muhammad; Liu, Dandan; Abdulrahim, Yassin; Liu, Yunhuan; Qian, Gang; Khan, Alamzeb; Gan, Fang; Huang, Kehe

doi:10.1016/j.rvsc.2017.05.002

Cited by 67 publications

(43 citation statements)

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“…Paraffin embedding sections of liver were subjected to immunohistochemistry evaluation using primary mouse monoclonal antibody against α‐SMA and PCNA, and performed according to our previous publications (Hamid and others , ). The intensity of PCNA staining was analyzed in 5 random fields on each slide.…”

Section: Methodsmentioning

confidence: 99%

“…TRIzol (Invitrogen) was used to extract total RNA from liver tissue according to the manufacturer instructions. Real‐time PCR was conducted in ABI Prism 7300 Detection System (Applied Biosystems, U.S.A.), using Kits obtained from TaKaRa Biotechnology Co. All the reactions were performed according to our previous publications (Khan and others ; Hamid and others ). The quality of RNA was evaluated by both a NanoDrop ND‐1000 Spectrophotometer (Thermo Scientific, U.S.A.), and agarose gel (1%) electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

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The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

Hamid

Abdulrahim

Liu

et al. 2018

Journal of Food Science

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Chronic liver diseases are the serious health problems, which increase the morbidity and mortality in the world today. Selenium-enriched yeast (SY) and Gum Arabic (GA) combination might be potential dietary agents could obviously ameliorate chronic liver damage, higher than GA and SY alone. They act to suppress the inflammation and inhibit the profibrotic response as well as support the liver regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

Hamid

Abdulrahim

Liu

et al. 2018

Journal of Food Science

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“…Cytokines IL-1 β , IL-2, IL-6, and TNF α are produced by activation of macrophages, and they are involved in the upregulation of inflammation. [ 6 ]. Apoptosis is the molecular process of cell death that occurs in organism in which programmed sequence of events leads to the elimination of cell without releasing harmful substance in surrounding area.…”

Section: Introductionmentioning

confidence: 99%

Nephroprotective Effect of Zingerone against CCl₄‐Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism

Safhi

2018

Oxidative Medicine and Cellular Longevity

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The protective effects of Zingerone against CCl4 induced nephrotoxicity in Swiss albino mice via modulation of metabolizing enzyme, oxidative stress, inflammatory cytokines, and apoptosis. The biochemical estimation indicated that the BUN and creatinine were significantly increased in group 2 (CCl4) compared to group 1 (normal) which was significantly reduced after treatment with Zingerone in group 3 when compared with group 2. The CCl4 treatment has significantly increased TBARS levels and reduced the antioxidant enzyme such as GSH, GPx, GR, GST, CAT, and SOD in group 2 compared to group 1, while the Zingerone treatment showed significant reduction in TBARS levels and increased the antioxidant enzymes in group 3 (CCl4 + Zingerone) as compared to group 2. Similarly, it was observed that CCl4 significantly increased the cytokines such as IL-1β, IL-2, and TNFα levels in group 2 as compared to group 1. The treatment with Zingerone significantly attenuated the levels of IL-1β, IL-2, and TNFα in group 3 compared to group 2. Caspase 3 and caspase 9 were also significantly increased in CCl4-treated group 2, whereas Zingerone treatment significantly reduced the elevated levels of caspases 3 and 9 in group 3 compared to group 2.

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“…Astragalus membranaceus is the main component of Fangji Huangqi decoction. Pharmacological studies have shown that APS, as the main ingredient of Astragalus membranaceus, has various therapeutic functions, such as immune regulation [20,21], anti-inflammation [7,22], antioxidation [23], antivirus [24,25], antibacterial [26], lowering blood sugar [27], and protecting the liver [28] and kidney [29]. Furthermore, it has been reported that APS could prevent RA progression [8,30].…”

Section: Resultsmentioning

confidence: 99%

Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides

Cao

Wang

et al. 2019

International Journal of Polymer Science

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This study investigated the effect of cellulase on the isolation of crude Astragalus polysaccharide (APS), analyzed the monosaccharide component of deproteinized APS, detected the molecular weights of purified APS, and examined the biological activities and the preliminary mechanism against rheumatoid arthritis (RA). Compared with water extraction method, cellulase-assisted extraction increased the yield of crude APS to 154% and polysaccharide contents to 121%. Crude APS was then purified by ethanol precipitation, Sevag deproteinization, and high-performance liquid chromatography (HPLC) analysis; monosaccharide contents of APS were different after cellulase-assisted method, especially galacturonic acid content which significantly increased. DEAE-52 cellulose column chromatography isolated three polysaccharide fractions, including a neutral polysaccharide (APS-water) and two acidic polysaccharides (APS-NaCl1 and APS-NaCl2). Using high-performance gel permeation chromatography (HPGPC), the molecular weights of APS-water, APS-NaCl1, and APS-NaCl2 were identified as 67.7 kDa, 234.1 kDa, and 189.4 kDa, respectively. Then their therapeutic effects and possible mechanism against RA were explored using type II collagen-induced arthritis (CIA) rat model. APS could significantly reduce paw swelling, serum concentration of IL-1β and TNF-α, and the expression levels of NF-κB-p65 and IκBα in synovial membranes in CIA rats. Our study indicated that cellulase significantly increases the yield and polysaccharide contents of crude APS, improves the product quality, and preserves the biological features against RA in CIA rats.

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Amelioration of CCl4-induced liver injury in rats by selenizing Astragalus polysaccharides: Role of proinflammatory cytokines, oxidative stress and hepatic stellate cells

Cited by 67 publications

References 50 publications

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

Nephroprotective Effect of Zingerone against CCl₄‐Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism

Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides

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Amelioration of CCl4-induced liver injury in rats by selenizing Astragalus polysaccharides: Role of proinflammatory cytokines, oxidative stress and hepatic stellate cells

Cited by 67 publications

References 50 publications

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

The Hepatoprotective Effect of Selenium‐Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride‐Induced Chronic Liver Injury in Rats

Nephroprotective Effect of Zingerone against CCl4‐Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism

Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides

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Product

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Nephroprotective Effect of Zingerone against CCl₄‐Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism