Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Patel, Roshni; Kadri, Shahrukh; Gohil, Priyanshee; Deshpande, Shrikalp; Shah, Gaurang

doi:10.1186/s43094-021-00361-w

Cited by 10 publications

(7 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the positive benefits of the inhibition of NO production were inferred indirectly in RA patients using glucocorticoids, salicylates, indomethacin, and methotrexate. 30 The present study also demonstrated a significant increase in iNOS expression at the RNA level and nitric oxide (NO) concentration in the blood of experimentally induced arthritic rats. Daily administration of trans -chalcone to arthritic rats for 28 days had a prominent inhibitory effect on the production of iNOS, and the blood nitric oxide level strengthened its protective role in rheumatoid arthritis.…”

Section: Discussionsupporting

confidence: 70%

“…Preclinical investigations indicated significantly increased NO levels in experimentally generated arthritic rats and restored such levels by selective inhibitors. Additionally, the positive benefits of the inhibition of NO production were inferred indirectly in RA patients using glucocorticoids, salicylates, indomethacin, and methotrexate . The present study also demonstrated a significant increase in iNOS expression at the RNA level and nitric oxide (NO) concentration in the blood of experimentally induced arthritic rats.…”

Section: Discussionsupporting

confidence: 59%

“…The functional degradation of an animal’s ability to walk is likewise linked to arthritis. 30 , 31 The present research came out with evidence of significant joint stiffness, marked flexion pain, impairment of mobility, and a lower stance score in CFA-injected rats compared to normal animals. However, the administration of trans -chalcone to animals considerably mitigated joint stiffness, relieved flexion pain, and improved mobility and stance.…”

Section: Discussionmentioning

confidence: 59%

See 2 more Smart Citations

Trans-Chalcone (1–3-diphenyl-2-propen-1-one) as a Therapeutic Candidate in Joint Inflammation via Reduction of TNF-α, IL-1β, IL-6, and IL-17 in Rodents: An In Vivo Study by RT-PCR and ELISA analysis

Jabbar,

Irfan,

Alamgeer

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Autoimmune disorders include vast and distinct illnesses and are characterized by an immune system-mediated attack on the body’s own tissues. Because of their ability to impact any portion of the body, their clinical symptoms are incredibly varied. The variations in symptoms are normally linked with the release and activation of vasoactive, chemotactic substances and cytokines. Cytokines perform a multitude of vital biological tasks, such as immune response control, inflammation, proliferation, and tissue repair. The reversal of inflammatory cytokines and leukocyte infiltration into the inflamed tissue by natural compounds provides an effective remedy for autoimmune diseases. Here, the oral administration of trans-chalcone (TC) for 28 days was tested with gradually increasing doses (30, 60, and 120 mg/kg) in complete Freund’s adjuvant (CFA)-provoked joint tissue stiffness in rats. Paw edema, arthritic index, joint stiffness, thermal and flexion pain, C-reactive protein, and rheumatoid factor (RF) levels were determined to check the tested drug effectiveness in a chronic inflammatory model. Molecular docking studies revealed strong binding affinity with inflammatory cytokines and mediators such as TNF-α, IL-17, COX-2, and iNOS; further, they were quantified at the mRNA level by RT-PCR and ELISA analysis. Oral administration of TC significantly ameliorated paw edema, thymus and spleen indices, joint stiffness, thermal and flexion pain, C-reactive protein, RF, mobility, and stance of the treated animals. This therapeutic effectiveness was linked with a reduction in the mRNA expression of proinflammatory cytokines such as IL-1β, IL-6, and IL-17. The findings of the reported research confirmed the effectiveness of TC in ameliorating joint stiffness and flexion pain by prominently lowering the inflammatory cytokines.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 59%

See 1 more Smart Citation

Trans-Chalcone (1–3-diphenyl-2-propen-1-one) as a Therapeutic Candidate in Joint Inflammation via Reduction of TNF-α, IL-1β, IL-6, and IL-17 in Rodents: An In Vivo Study by RT-PCR and ELISA analysis

Jabbar,

Irfan,

Alamgeer

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“…CFA-induced arthritis is a chronic animal model that is the most widely used in rheumatoid arthritis experimental models (Noh et al, 2021;Patel et al, 2021). The increasing volume of the injected leg indicates chronic inflammation in the CFA model.…”

Section: Resultsmentioning

confidence: 99%

Identification Bioactive Compound of Ethanol-Water Fraction of Coleus atropurpureus for Anti-rheumatic Rheumatism in CFA-induced Rats

Djunarko

Fakhrudin

Nurrochmad

et al. 2022

J.Pharm.Sci.Community

View full text Add to dashboard Cite

Nonsteroidal anti-inflammatory drugs are used for pain and to slow the progression of rheumatoid arthritis. Accordingly, the discovery of rheumatoid arthritis active compounds from the ethanol-water fraction Coleus atropurpureus (EWC) was conducted to characterize the isolated compounds as well as the anti-rheumatic effects of the EWC induced Complete Freund's Adjuvant (CFA). We conducted in vivo study in rats which were randomly divided into 5 groups. Group 1 was only given CFA as a negative control. Group 2 as positive control was orally exposed to diclofenac potassium (9 mg/BW). Three groups were given different EWCs orally as follows: 11.25 mg/BW, 22.5 mg/BW, and 45 mg/BW, respectively. Rheumatism rates were then compared with positive controls using a visual arthritic scoring system. The compounds identified by isolation of the EWC of Coleus atropurpureus predicted forskolin. The ethanol-water fraction Coleus atropurpureus did not act as an anti-rheumatic arthritis agent in CFA-induced rats.

show abstract

“…Experimental rats had arthritis induced by injecting a 0.1 ml (0.1% w/v) slurry of deceased Mycobacterium tuberculosis bacteria homogenised in liquid paraffin (Fruend's complete adjuvant) into their right hind paws. On day 14, following the induction of chronic disease, drug treatment began and lasted for 28 days (Patel et al 2021, Nipate et al 2015.…”

Section: Fca-induced Arthritismentioning

confidence: 99%

Anti-arthritic potential of ethyl acetate fraction of <em>Pinus roxburghii</em> Sargent stem bark in Freund's complete adjuvant induced arthritis in Wistar rats

Hajare,

Rai,

Nipate

et al. 2023

Multidiscip. Sci. J.

View full text Add to dashboard Cite

Traditional and folk medicine regularly make use of the Pinus roxburghii Sargent (Pinaceae) plant. Unfortunately, there is a lack of data on this plant's potential anti-arthritic effects.Pinus roxburghii Sargent ethyl acetate stem bark has anti-arthritic properties in Wistar rats with arthritis produced by Freund's Complete adjuvant. As a positive control, diclofenac was administered. To define the anti-arthritic activity, the influence on pain perception parameter, haematological parameters, the effect on rheumatoid factor, CRP, and serum parameters were evaluated.In this study, the ethyl acetate fraction of Pinus roxburgii Sargent was found to significantly inhibit pain perception parameters at doses of 250 and 500 mg/kg. Serum levels of RF, NO, and CRP were significantly lower in the Pinus roxburghii Sargent group compared to the disease control group. In disease-free rats, Pinus roxburghii Sargent significantly raised RBC and lowered WBC, both of which point to a suppressive effect on inflammatory mediators. The number of inflammatory cells had decreased, as seen by histopathology. Several imaging parameters, including spur formation, joint spacing, bone erosion, etc., were significantly reduced in a radiological assessment.In this study, authors noted that Pinus roxburghii Sargent had a direct effect on slowing the progression of arthritis, reducing inflammation and synovitis, and protecting arthritic joints from cartilage and bone deterioration. Stem bark extract of Pinus roxburghii Sargent has been shown to have anti-arthritic action based on clinical, biochemical, and histological analysis. Yet more research is required to pin down the precise mechanism of action.

show abstract

Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Cited by 10 publications

References 65 publications

Trans-Chalcone (1–3-diphenyl-2-propen-1-one) as a Therapeutic Candidate in Joint Inflammation via Reduction of TNF-α, IL-1β, IL-6, and IL-17 in Rodents: An In Vivo Study by RT-PCR and ELISA analysis

Trans-Chalcone (1–3-diphenyl-2-propen-1-one) as a Therapeutic Candidate in Joint Inflammation via Reduction of TNF-α, IL-1β, IL-6, and IL-17 in Rodents: An In Vivo Study by RT-PCR and ELISA analysis

Identification Bioactive Compound of Ethanol-Water Fraction of Coleus atropurpureus for Anti-rheumatic Rheumatism in CFA-induced Rats

Anti-arthritic potential of ethyl acetate fraction of <em>Pinus roxburghii</em> Sargent stem bark in Freund's complete adjuvant induced arthritis in Wistar rats

Contact Info

Product

Resources

About