Ameliorative Effect of Carob Pods Extract (Ceratonia siliqua L.) against Cyclophosphamide Induced Alterations in Bone Marrow and Spleen of Rats

doi:10.7324/japs.2017.71025

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Our results are in accordance with other studies that showed a reduction in lymphoid cellularity of the spleen in CTX-treated rats (Senin et al, 2020). Moreover, the greatest cyclophosphamide cytotoxicity against cells of the immune and hematopoietic systems was due to the active and continuous replication of their DNA resulting in serious cytotoxic damage (Abd Elhalim et al, 2017).…”

Section: Discussionsupporting

confidence: 93%

“…Concerning the current work, using CD20, a marker of the B lymphocyte population, it revealed a weak reaction in the spleen of animals treated with CTX due to the severe damage of B lymphocytes in the white pulp. This can be attributed to the profound depletion of B cells because of dose-intensive cytotoxic antineoplastic therapy (Abd Elhalim et al, 2017). Moreover, Al-Joufi et al ( 2022) findings suggested that CTX suppresses the lymphocyte count and function in the spleen and Peyer's patches of the ileum in a timedependent fashion, especially B cells which could lead eventually to bacteraemia.…”

Section: Discussionmentioning

confidence: 99%

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Protective effect of Pentoxifylline versus Nigella sativa against Cyclophosphamide induced splenic damage in adult male albino rats.

Fekry

Awny

Makary

et al. 2023

Mansoura Journal of Forensic Medicine and Clinical Toxicology

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Cyclophosphamide (CTX) is an effective anticancer therapy. It is known to cause oxidative stress and cytotoxic effects, especially on the immune system. Pentoxifylline and Nigella sativa seeds both possess antioxidant and anti-inflammatory properties. This study explores the ameliorative impact of both pentoxifylline and nigella sativa over the splenic damage induced by cyclophosphamide. Thirty-six adult male albino rats were separated into six groups from six each and received treatment for 10 days. Group I (control) received saline 0.9% solution orally via intragastric tube, group II received pentoxifylline (150 mg/kg) orally, group III received nigella sativa oil (20 mg/day) orally, group IV received a single intraperitoneal injection (IP) of CTX (200 mg/kg), group V received pentoxifylline (150 mg/day) orally for 10 days and at the 8thday animals received single IP injection of CTX (200 mg/kg), group VI received nigella sativa oil (20 mg/day) orally for 10 days and similarly at the 8th-day animals received single IP injection of CTX (200 mg/kg). Light microscopic and ultrastructure changes in the spleen of the different experimental groups were examined. CTX induced splenic damage in the form of a disrupted and detached capsule of the spleen, lymphocyte loss with marked thickening and dilatation of the central artery in the white pulp, and cellular loss and congested blood sinusoids in the red pulp. The use of pentoxifylline and nigella sativa resulted in the restoration of splenic tissue. Pentoxifylline showed a more protective effect than nigella sativa on the splenic tissue against CTX-induced damage.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Protective effect of Pentoxifylline versus Nigella sativa against Cyclophosphamide induced splenic damage in adult male albino rats.

Fekry

Awny

Makary

et al. 2023

Mansoura Journal of Forensic Medicine and Clinical Toxicology

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Assessment of the Ameliorative Effect of Bacillus subtilis against the Toxicity Induced by Aflatoxin B1 in Rats

et al. 2021

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Aflatoxin B1 (AFB1) is the most toxic and common among the major types of aflatoxins. AFB1 is hepatoxic and has been implicated in an increase risk of hepatocellular carcinoma, also the effect of AFs on the brain chemistry have been documented. Probiotics have health benefits and provide of powerful benefits for the body and brain. The present study was designed to reveal efficiency of Bacillus subtilis NS4182-01 against the hematological, biochemical and histopathological alterations induced by AFB1 in rats. Both of AFB1 and B. subtilis were studied on the experimental animals (rats) that were divided into 6 groups as the following: Group 1 (control) received distilled water orally. Groups 2&3 (B. subtilis treated groups) received B. subtilis orally at a dose of 0.25 and 0.50 ml, respectively. Group 4 (AFB1 treated group) orally treated with AFB1 at a dose of 2 mg/kg b.w. Groups 5&6 (AFB1 + B. subtilis group) orally treated with AFB1 and then treated with B. subtilis at two studied doses respectively. It was noticed that the hematological measurements declined and the most biochemical measurements elevated significantly (P≤0.05) in AFB1 treated group. B. subtilis restored all studied measurements towards the normal values. Moreover, the native electrophoretic protein patterns showed that the physiological alterations occurred in the native protein and lipid moiety in addition to calcium moiety of native protein patterns as a result of AFB1 treatment were represented by hiding one or more of normal protein types with existence of abnormal ones. Therefore, the similarity index (SI%) and genetic distance (GD%) values were altered with protein (SI=40.00%; GD=60.00%), lipid moiety (SI=50.00%; GD=50.00%) and calcium moiety of native protein patterns (SI=75.00%; GD=25.00%) in AFB1 treated group. Although the treatment with B. subtilis at a dose of 0.25 ml showed ameliorative effect but could not restore the physiological state of the patterns to normalcy. While B. subtilis at a dose of 0.50 ml restored integrity of these native protein patterns by restoring the absent types with hiding the abnormal ones. Therefore, this group became physiologically similar to control group (SI=100.00%; GD=0.00%). These findings were supported by results of the histopathological examination in the most target organs (liver, kidney and brain) that were affected by AFB1 and the B. subtilis restored their histopathological integrity to normal structure and maintained architecture of these organs.

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Evaluation of the Biological Efficiency of Silver Nanoparticles Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced Colon Cancer in Rats

Aboulthana

Ibrahim

Osman

et al. 2020

Asian Pac J Cancer Prev

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Background: Colorectal cancer (CRC) is considered as the most common type of gastrointestinal cancers. Chemotherapy became limited due to the adverse side effects. Therefore, the most effective Croton tiglium extract was selected to be incorporated by silver nanoparticles (Ag-NPs) then evaluated against colon cancer induced by azoxymethane (AOM) in rats. Methods: Different hematological and biochemical measurements were quantified in addition to markers of oxidative stress. Specific tumor and inflammatory markers were assayed. Colonic tissues were examined histopathologically in addition to immunohistochemistry (IHC). Native proteins and isoenzymes patterns were electrophoretically assayed beside expression of Tumor Protein P 53 (TP 53) and Adenomatous Polyposis Coli (APC) genes in colonic tissues. Results: It was found that AOM caused significant (P≤0.05) elevation in the hematological and biochemical measurements. C. tiglium nano-extract restored these measurements to normalcy. Tumor and inflammatory markers elevated significantly (P≤0.05) in sera of AOM induced colon cancer group in addition to increasing peroxidation products with decline in antioxidant enzymes activities in colon tissues. Nano-extract restored these measurements to normalcy in post-treated group. Histopathological study revealed that nano-extract minimized severity of inflammatory reactions in all nano-extract treated groups and prevented anti-Keratin 20 antibody expression in post-treated group. The lowest similarity index (SI%) values were noticed with electrophoretic protein (SI=71.43%), lipid (SI=0.00%) and calcium (SI=75.00%) moieties of protein patterns, catalase (SI=85.71%), peroxidase (SI=85.71%), α-esterase (SI=50.00%) and β-esterase (SI=50.00%) isoenzymes in colon cancer group. Furthermore, AOM altered the relative quantities of total native bands. The nano-extract prevented the alterations that occurred qualitatively in nano-extract post-treated group and quantitatively in all nano-extract treated groups. Levels of TP 53 and APC gene expression increased in AOM injected group and nano-extract restored their levels to normalcy in the post-treated group. Conclusion: C. tiglium nano-extract exhibited ameliorative effect against the biochemical and molecular alterations induced by AOM in nano-extract post-treated group.

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Ameliorative Effect of Carob Pods Extract (Ceratonia siliqua L.) against Cyclophosphamide Induced Alterations in Bone Marrow and Spleen of Rats

Cited by 4 publications

References 20 publications

Protective effect of Pentoxifylline versus Nigella sativa against Cyclophosphamide induced splenic damage in adult male albino rats.

Protective effect of Pentoxifylline versus Nigella sativa against Cyclophosphamide induced splenic damage in adult male albino rats.

Assessment of the Ameliorative Effect of Bacillus subtilis against the Toxicity Induced by Aflatoxin B1 in Rats

Evaluation of the Biological Efficiency of Silver Nanoparticles Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced Colon Cancer in Rats

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