Ameliorative Effects of Peptides from the Oyster (Crassostrea hongkongensis) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice

Abstract

Chronic exposure to ultraviolet B (UVB) irradiation is a major cause for skin photoaging. UVB induces damage to skin mainly by oxidative stress, inflammation, and collagen degradation. This paper investigated the photo-protective effects of peptides from oyster (Crassostrea hongkongensis) protein hydrolysates (OPs) by topical application on the skin of UVB-irradiated mice. Results from mass spectrometry showed that OPs consisted of peptides with a molecular weight range of 302.17–2936.43 Da. In vivo study demonstrated that topical application of OPs on the skin significantly alleviated moisture loss, epidermal hyperplasia, as well as degradation of collagen and elastin fibers caused by chronic UVB irradiation. In this study, OPs treatment promoted antioxidant enzymes (SOD and GPH-Px) activities, while decreased malondialdehyde (MDA) level in the skin. In addition, OPs treatment significantly decreased inflammatory cytokines (IL-1β, IL-6, TNF-α) content, and inhibited inflammation related (iNOS, COX-2) protein expression in the skin. Via inhibiting metalloproteinase 1(MMP1) expression, OPs treatment markedly decreased the degradation of collagen and elastin fibers as well as recovered the altered arrangement of extracellular matrix network in the dermis of skin. Our study demonstrated for the first time that OPs protected against UVB induced skin photodamage by virtue of its antioxidative and anti-inflammatory properties, as well as regulating the abnormal expression of MMP-1. The possible molecular mechanism underlying OPs anti-photoaging is possibly related to downregulating of the MAPK/NF-κB signaling pathway, while promoting TGF-β production in the skin.

Collagen Peptides Derived from Sipunculus nudus Accelerate Wound Healing

“…The amino acid of the SNCP was determined according to the previous study [ 56 ]. SNCP was hydrolyzed by dissolving it in 6 M HCl at 110 °C for 24 h. The filtrate and standard samples of amino acids solution was analyzed with an automatic amino acid analyzer (S-433D, Sykam, Bremen, Germany).…”

The Potential Protective Effect and Possible Mechanism of Peptides from Oyster (Crassostrea hongkongensis) Hydrolysate on Triptolide-Induced Testis Injury in Male Mice

“…OPs were prepared by enzymatic hydrolysis from the oyster meat according to the methods of Li et al, Peng et al, and Zhang et al [ 17 , 41 , 47 ]. Briefly, three kilograms of oyster meat were ground into mince and then mixed with distilled water (1:3 w / v ).…”

“…Sodium citrate buffer solution was added to the dried test tube and dissolved. The solution was transferred to the injection flask of the instrument for determination by the amino acid analyzer [ 47 ]. According to the peak area in comparison with the standard, amino acid contents were calculated.…”

Fish and Shellfish-Derived Anti-Inflammatory Protein Products: Properties and Mechanisms

“…In a study by Kaul et al in vitro evidence suggests that L-proline acts as a radical scavenger [ 60 ]. There is evidence that cysteine may act as a radical scavenger as well [ 44 , 51 , 61 , 62 ], and cysteine has been implicated as an AA residue abundant in numerous anti-inflammatory BAPs [ 48 ]. One potential mechanism of cysteine’s anti-inflammatory activity is IL-15Rα binding and agonism [ 29 ].…”

“…One potential mechanism of cysteine’s anti-inflammatory activity is IL-15Rα binding and agonism [ 29 ]. A study by Peng et al [ 62 ] found that hydrolysates high in radical scavenging peptides including cysteine, methionine, and tryptophan ameliorated the effects of UVB radiation in mice models by increasing enzyme activity of superoxide dismutase (SOD), and glutathione peroxidase (Gpx), while decreasing levels of malondialdehyde, IL-1β, IL-6, TNF-α, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in mice models when applied topically.…”