Ameliorative potential of Blighia sapida K.D. Koenig bark against pancreatic β-cell dysfunction in alloxan-induced diabetic rats

Abstract

Background In West Africa, the fruit, seed, leaf and stem of Blighia sapida K.D. Koenig are commonly used as remedy against a variety of diseases, including diabetes mellitus. This study investigated the ameliorative potential of B. sapida K.D. Koenig stem bark ethanol extract against pancreatic β-cell dysfunction in diabetic rats. Methods Diabetes was induced by intraperitoneal injection of alloxan (65 mg/kg body weight) for 21 days, and orally administered with glibenclamide (5 mg/kg body weight), 50-150 mg/kg body weight of B. sapida stem bark ethanol extract once daily for 21 days. Results The blood glucose levels of rats induced with alloxan were significantly and gradually reduced (p<0.05) in B. sapida stem bark ethanol extract treated animals at the dose of 50-150 mg/kg body weight, and in glibenclamide-treated animals. The significant increase in the lipid peroxidation (malonaldehyde), homeostasis model assessment-insulin resistance scores (HOMA-IR) and decrease in serum insulin, pancreatic β-cell scores as well as antioxidant marker enzymes in untreated diabetic rats compared to normal control rats were reversed by the B. sapida stem bark ethanol extract and glibenclamide. Similarly, histopathological changes in the pancreas were also reversed by the extract and glibenclamide. However, these effects were most prominent in the animals treated with 150 mg/kg body weight of B. sapida bark. Conclusions These findings indicate that B. sapida stem bark possess anti-hyperglycemic activity and exhibits ameliorative potential in managing diabetes.

Aqueous extract of Carica papaya Linn. roots potentially attenuates arsenic induced biochemical and genotoxic effects in Wistar rats

“…Treatment with C. papaya root aqueous extract at 100 mg/kg and 150 mg/kg body weight and vitamin C (200 mg/kg) significantly decreased chromosomal aberrations and, micro nuclei induction with an increase in the mitotic index. The types of cytogenetic damage detected in this work may be ascribed to many modes of arsenic toxicity such as inhibition of enzymes concerned in the repair of DNA and gene expression, 79 the increase of ROS levels capable of inflicting damage on biopolymers 80 , 81 , 82 or the induction of gene expression resulting in modification in repair mechanisms maintaining DNA integrity. 56 …”

Antioxidative Properties of Blighia sapida K.D. Koenig Stem Bark Extract and Inhibitory Effects on Carbohydrate Hydrolyzing Enzymes Associated with Non-Insulin Dependent Diabetes Mellitus

“…Koenig were collected at a local farm Abeokuta, Ogun State. Identification and authentication was previously done by (Ojo et al, 2017).…”

“…B. sapida root extract has been shown to have hypoglycemic effect in normoglycemic rats (Saidu et al 2012). B. sapida bark extract have been shown to ameliorate pancreatic β-cell dysfunction (Ojo et al, 2017). However, an attempt at understanding the pharmacological features of these plants with their different chemical compound that may hitherto poses hypoglycemic potentials necessitated this study.…”

Antihyperlipidemic Activities and Hematological Properties of Ethanol Extract of Blighia Sapida Koenig Bark in Alloxan-Induced Diabetic Rats

“…Koenig is distributed throughout Nigeria [10]. B. sapida could be a therapeutic herbal plant ordinarily utilized by trado-medical healers in Nigeria, and highly appreciated in West Africa for the management of various diseases like diabetes mellitus [11]. The fruit has inhibitory effect against α-glucosidase and α-amylase as reported by Kazeem et al [12].…”