Ameliorative potential of Psidium guajava on hemato-biochemical alterations in arsenic-exposed Wistar rats

Abstract

The present study attempts to investigate the effects of Psidium guajava (P. guajava) when administered in combination with sodium arsenite @ 20 ppm in drinking water with the aim of achieving normalization of altered biochemical, hematological parameters suggestive of hepatic damage and depletion of inorganic arsenic following chronic arsenic exposure. Thirty adult Wistar rats were given 20 ppm arsenic for eight weeks along with hydro alcoholic leaf extract of P. guajava at a dose of 100 mg/kg body weight wt. (orally) (once daily for eight weeks). Arsenic exposure led to significant depletion of hemoglobin, red blood cells (RBC) and packed cell volume (PCV) but elevated leucocyte count (TLC). There was a significant increase (P<0.01/P<0.05) in serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphotase (ALP), acid phosphotase (ACP) and blood glucose whereas decrease in total protein level in arsenic-exposed untreated animals. The changes were accompanied by a significant elevation in blood and soft-tissue arsenic concentration. Co-administration of P. guajava was most effective not only in reducing arsenic-induced hematological and biochemical alterations but also in depleting arsenic from blood and soft tissues following arsenic exposure. We thus recommend combined leaf extract of P. guajava for achieving optimum effects of chelation therapy.

In VivoEffect of Arsenic Trioxide on Keap1-p62-Nrf2 Signaling Pathway in Mouse Liver: Expression of Antioxidant Responsive Element-Driven Genes Related to Glutathione Metabolism

“…The reason for such reversal is difficult to explain since histopathological alterations as well as induction of apoptosis was observed in the same treatment group. The increased SGOT and SGPT levels in rat after 45 days treatment of 10 ppm sodium arsenite was also described by Tandan et al [ 47 ], whereas Santra et al [ 45 ] reported increased SGOT and SGPT levels after 12 months of arsenic treatment at 3.2 ppm in mice. Liver alteration in histopathological architecture was evident mainly in the form of loss of integrity of central vein, loss of hepatic cord organization, and dose and time dependent vacuolation which corroborates with earlier studies [ 45 , 48 , 49 ].…”

The Hepatorenal protective potential of caffeic acid consumption on the Arsenic-exposed Syrian mice

“…There are several types of herbal-based chelators have been used as the arsenic antidote such as Cucumis Sativus [39], curcumin [40], green tea [41], and Psidium guajava [42].…”

Ameliorative effect of tephrosia purpurea in arsenic-induced nephrotoxicity in rats

“…The increase of creatinine concentration might be due to the loss of of kidney function and considered as functional evidence of arsenic-induced nephrotoxicity. [ 24 25 26 ] There was a decrease in serum albumin in rats of group I compared to the control group which might be due to the severe nephrotoxic effect of arsenic resultinga drainage of protein through urine. There was a significant decrease in BUN and creatinine levels in the Tephrosia–treated (group II) animals [ Table 1 ] with respect to group I.…”