Ameliorative role of inducible nitric oxide synthase inhibitors against sodium arsenite-induced renal and hepatic dysfunction in rats

Sharma, Ashwani Kumar; Kaur, Anmoldeep; Kaur, Tajpreet; Kaur, Sarabjit; Pathak, Devendra; Singh, Amrit Pal

doi:10.1080/01480545.2021.1926109

Cited by 6 publications

(2 citation statements)

References 51 publications

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“…Kidney sections were stained with Hematoxylin and Eosin (H&E) stain, Sirius Red stain, and Toluidine Blue stain, as well as different antibodies IL-33 (1:400, Affinity Biosciences, Cincinnati, OH, USA), NLRP3 (1:400, Novus Biologicals, Centennial, CO, USA), and IL-1β (1:400, Abcam, Cambridge, UK), and the expression changes were recorded using an EVOS ® FL Auto Imaging System (Thermo Fisher Scientific, Waltham, MA, USA). The determination of tubulointerstitial injury, degree of interstitial collagen deposition, and tubulointerstitial infiltration was based on previous literature [ 28 , 33 , 34 ].…”

Section: Methodsmentioning

confidence: 99%

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo

et al. 2022

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Lupus nephritis (LN) is a common and serious symptom in patients with systemic lupus erythematosus (SLE). Tubular interstitial fibrosis is a common underlying mechanism in the development of lupus nephritis to end-stage renal failure (ESRD). Quercetin is widely proven to prevent tissue fibrosis. Therefore, the purpose of this study is to investigate the beneficial effects of quercetin on the inhibition of fibrosis and inflammation pathways in in vitro and in vivo lupus nephritis models. In the current study, MRL/lpr mice as animal models, and HK-2 human renal tubular epithelial cells were stimulated by interleukin-33 (IL-33) to mimic the cellular model of lupus nephritis. Immunohistochemical staining, immunoblotting assay, immunofluorescence staining, and quantitative real-time polymerase chain reaction assay were used. The in vivo results showed that quercetin improved the renal function and inhibited both fibrosis- and inflammation-related markers in MRL/lpr mice animal models. The in vitro results indicated that quercetin ameliorated the accumulation of fibrosis- and inflammation-related proteins in IL-33-induced HK-2 cells and improved renal cell pyroptosis via the IL33/ST2 pathway. Overall, quercetin can improve LN-related renal fibrosis and inflammation, which may offer an effective potential therapeutic strategy for lupus nephritis.

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Section: Methodsmentioning

confidence: 99%

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo

et al. 2022

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“…In addition, compelling literature has revealed positive corrections between arsenical exposure and nephrotoxicity by promoting oxidative stress-related damage, inflammation, and apoptosis. [13][14][15] Moreover, González-Cortés et al confirmed that arsenic exposure disrupted the mammalian extracellular matrix (ECM) remodeling process by altering the expression patterns of genes including matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), 16 in turn, resulted in an imbalance between excessive synthesis and reduced ECM component breakdown and triggered progressive renal fibrosis and renal dysfunctions including glomerulosclerosis and end-stage renal diseases. 17,18 Over the past decades, natural or synthetic antioxidants such as naringenin, 19 sodium selenite (Na 2 SeO 3 ), 20,21 betaine, 15 bosentan, 14 tetramethylpyrazine, 22 and chlorogenic acid 23 have been used to combat arsenic exposure-induced nephrotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Nephroprotective Effects of Selenium Nanoparticles Against Sodium Arsenite-Induced Damages

Li¹,

Dong²,

Xu³

et al. 2023

IJN

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Introduction The potential effects of selenium nanoparticles (SeNPs) administration on arsenic exposure-mediated nephrotoxicity by alleviating fibrosis, inflammation, oxidative stress-related damage, and apoptosis remains more detailed investigations. Methods After the synthesis of selenium nanoparticles (SeNPs) by sodium selenite (Na 2 SeO 3 ) through a versatile and green procedure, the biosafety of SeNPs was assessed by assaying renal functions and inflammation in mice. Subsequently, nephroprotective effects of SeNPs against sodium arsenite (NaAsO 2 )-induced damages were confirmed by biochemical, molecular, and histopathological assays, including renal function, histological lesion, fibrosis, inflammation, oxidative stress-related damage, and apoptosis in mice renal tissues and renal tubular duct epithelial cells (HK2 cells). Results The excellent biocompatibility and safety of SeNPs prepared in this study were confirmed by the non-significant differences in the renal functions and inflammation levels in mice between the negative control (NC) and 1 mg/kg SeNPs groups (p>0.05). The results of biochemical, molecular, and histopathological assays confirmed that daily administration of 1 mg/kg SeNPs for 4 weeks not only ameliorated renal dysfunctions and injuries caused by NaAsO 2 exposure but also inhibited the fibrosis, inflammation, oxidative stress-related damage, and apoptosis in the renal tissues of NaAsO 2 -exposed mice. In addition, altered viability, inflammation, oxidative stress-related damage, and apoptosis in the NaAsO 2 -exposed HK2 cells were effectively reversed after 100 μg/mL SeNPs supplementation. Conclusion Our findings authentically confirmed the biosafety and nephroprotective effects of SeNPs against NaAsO 2 exposure-induced damages by alleviating inflammation, oxidative stress-related damage, and apoptosis.

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Arsenic-induced developmental neurotoxicity

Luo¹,

Shu²

2023

Handbook of Arsenic Toxicology

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Ameliorative role of inducible nitric oxide synthase inhibitors against sodium arsenite-induced renal and hepatic dysfunction in rats

Cited by 6 publications

References 51 publications

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo

Nephroprotective Effects of Selenium Nanoparticles Against Sodium Arsenite-Induced Damages

Arsenic-induced developmental neurotoxicity

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