Amelogenin Exon 5 Peptide Promotes Cell Proliferation and Osteogenic Differentiation in Human Dental Pulp Stem Cells

Kato, Hirohito; Taguchi, Yoichiro; Yamawaki, Isao; Ruan, Yaru; Wu, Qingchao; Nakano, Yuji; Tsumori, Norimasa; Nakata, Takaya; Noguchi, Masahiro; Umeda, Makoto

doi:10.3390/app9204425

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…In the osteogenic group, COL1A1 and MEPE , which have roles in hard tissue formation, were evaluated. As shown in Figure 8 b, the mRNA expression of COL1A1 , an early marker of bone formation [ 29 ], was significantly decreased in the BMP-2-treated group, in agreement with previous studies [ 30 , 31 ]. In contrast, the mRNA levels of MEPE , usually upregulated during the maturation of osteoblasts into osteocytes [ 32 ], was increased in the BMP-2-treated group.…”

Section: Resultssupporting

confidence: 90%

Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

Lee

Choi

et al. 2020

Polymers

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Osteochondral defects, including damage to both the articular cartilage and the subchondral bone, are challenging to repair. Although many technological advancements have been made in recent years, there are technical difficulties in the engineering of cartilage and bone layers, simultaneously. Moreover, there is a great need for a valuable in vitro platform enabling the assessment of osteochondral tissues to reduce pre-operative risk. Three-dimensional (3D) bioprinting systems may be a promising approach for fabricating human tissues and organs. Here, we aimed to develop a polycaprolactone (PCL)/alginate bipartite hybrid scaffold using a multihead 3D bioprinting system. The hybrid scaffold was composed of PCL, which could improve the mechanical properties of the construct, and alginate, encapsulating progenitor cells that could differentiate into cartilage and bone. To differentiate the bipartite hybrid scaffold into osteochondral tissue, a polydimethylsiloxane coculture system for osteochondral tissue (PCSOT) was designed and developed. Based on evaluation of the biological performance of the novel hybrid scaffold, the PCL/alginate bipartite scaffold was successfully fabricated; importantly, our findings suggest that this PCSOT system may be applicable as an in vitro platform for osteochondral tissue engineering.

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Section: Resultssupporting

confidence: 90%

Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

Lee

Choi

et al. 2020

Polymers

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“…Pluripotent Oct4 and Sox2 factors show higher expression levels in the apical papilla and can enhance pulp regeneration in the presence of this protein [40]. Other findings indicate that amelogenin exon 5 peptide promotes the expression of MAPK signaling pathway proteins, with enhanced cell proliferation and osteogenic differentiation [41]. The results of these studies are in agreement with the current findings; however, less is known about how amelogenin might influence DPSC odontoblastic differentiation and possible molecular mechanisms [42].…”

Section: Discussionsupporting

confidence: 87%

The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

2023

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The ideal treatment option for immature necrotic permanent teeth is regeneration of the pulp–dentin complex. Mineral trioxide aggregate (MTA), the conventional cement used for regenerative endodontic procedures, induces hard tissue repair. Various hydraulic calcium silicate cements (HCSCs) and enamel matrix derivative (EMD) also promote osteoblast proliferation. The purpose of the present study was to determine the osteogenic and dentinogenic potential of commercially distributed MTA and HCSCs when applied in combination with Emdogain gel on human dental pulp stem cells (hDPSCs). The presence of Emdogain resulted in greater cell viability, and higher alkaline phosphatase activity was detected in the Emdogain-supplemented groups in the early days of cell culture. On qRT–PCR, the groups treated, respectively, with Biodentine and Endocem MTA Premixed in the presence of Emdogain showed an increased expression of the dentin formation marker DSPP, and the group treated with Endocem MTA Premixed in the presence of Emdogain showed an upregulated expression of the bone formation markers OSX and RUNX2. In an Alizarin Red-S staining assay, all of the experimental groups exhibited a greater formation of calcium nodules when treated in combination with Emdogain. Overall, the cytotoxicity and osteogenic/odontogenic potential of HCSCs were similar to that of ProRoot MTA. The addition of the EMD increased the osteogenic and dentinogenic differentiation markers.

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“…The proliferation and differentiation of osteoblasts which requires stimulation from TGF-β, can be promoted by amelogenin. 53,54 But it will be inhibited by the local inflammatory microenvironment (Figure 8e,e1). 52 So the expression of TGF-β improved but not obvious in the hydrogel + amelogenin group.…”

Section: Resultsmentioning

confidence: 99%

Injectable and Self-Healing Hydrogels with Double-Dynamic Bond Tunable Mechanical, Gel–Sol Transition and Drug Delivery Properties for Promoting Periodontium Regeneration in Periodontitis

Guo

Huang

Yang

et al. 2021

ACS Appl. Mater. Interfaces

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Injection of a hydrogel loaded with drugs with simultaneous anti-inflammatory and tissue regenerating properties can be an effective treatment for promoting periodontal regeneration in periodontitis. Nevertheless, the design and preparation of an injectable hydrogel with self-healing properties for tunable sustained drug release is still highly desired. In this work, polysaccharidebased hydrogels were formed by a dynamic cross-linked network of dynamic Schiff base bonds and dynamic coordination bonds. The hydrogels showed a quick gelation process, injectability, and excellent self-healing properties. In particular, the hydrogels formed by a double-dynamic network would undergo a gel−sol transition process without external stimuli. And the gel−sol transition time could be tuned by the double-dynamic network structure for in situ stimuli involving a change in its own molecular structure. Moreover, the drug delivery properties were also tunable owing to the gel−sol transition process. Sustained drug release characteristics, which were ascribed to a diffusion process, were observed during the first stage of drug release, and complete drug release owing to the gel−sol transition process was achieved. The sustained drug release time could be tuned according to the double-dynamic bonds in the hydrogel. The CCK-8 assay was used to evaluate the cytotoxicity, and the result showed no cytotoxicity, indicating that the injectable and self-healing hydrogels with double-dynamic bond tunable gel−sol transition could be safely used in controlled drug delivery for periodontal disease therapy. Finally, the promotion of periodontal regeneration in periodontitis in vivo was investigated using hydrogels loaded with ginsenoside Rg1 and amelogenin. Micro-CT and histological analyses indicated that the hydrogels were promising candidates for addressing the practical needs of a tunable drug delivery method for promoting periodontal regeneration in periodontitis.

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Amelogenin Exon 5 Peptide Promotes Cell Proliferation and Osteogenic Differentiation in Human Dental Pulp Stem Cells

Cited by 4 publications

References 38 publications

Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System

The Combined Effects of Hydraulic Calcium Silicate Cement and Enamel Matrix Derivative Regarding Osteogenic and Dentinogenic Differentiation on Human Dental Pulp Stem Cells

Injectable and Self-Healing Hydrogels with Double-Dynamic Bond Tunable Mechanical, Gel–Sol Transition and Drug Delivery Properties for Promoting Periodontium Regeneration in Periodontitis

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