Amine Plasma-Polymerization of 3D Polycaprolactone/β-Tricalcium Phosphate Scaffold to Improving Osteogenic Differentiation In Vitro

Kim, Hee-Yeon; Kim, Byung–Hoon; Kim, Myung‐Sun

doi:10.3390/ma15010366

Cited by 15 publications

(16 citation statements)

References 67 publications

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“…Figure 1(a) depicts the fabrication process, while figure 1(b) shows the appearance of the scaffold. These PCL scaffolds were white and displayed excellent structural stability, in line with previous research [33]. The uniform texture across the surfaces confirmed successful fabrication of the intended designs.…”

Section: Preparation Of Scaffoldssupporting

confidence: 86%

“…Cell proliferation was assessed using the cell counting kit-8 (Boster, China). At specified intervals [33] (1, 3, and 5 d), a 10% CCK-8 solution was added to each well for a 2-hour incubation period. The supernatant was then transferred to a new 96-well plate, and the optical density (OD) was measured at 450 nm using a spectrophotometer (Spectrallax Plus 384, Germany).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

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3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration

Wang,

Fu,

Luo

et al. 2024

Biomed. Mater.

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Macrophage-mediated bone immune responses significantly influence the repair of bone defects when utilizing tissue-engineered scaffolds. Notably, the scaffolds' physical structure critically impacts macrophage polarization. The optimal pore size for facilitating bone repair remains a topic of debate due to the imprecision of traditional methods in controlling scaffold pore dimensions and spatial architecture. In this investigation, we utilized fused deposition modeling (FDM) technology to fabricate high-precision porous polycaprolactone (PCL) scaffolds, aiming to elucidate the impact of pore size on macrophage polarization. We assessed the scaffolds' mechanical attributes and biocompatibility. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression levels of macrophage-related genes, and enzyme linked immunosorbent assay (ELISA) for cytokine secretion levels. In vitro osteogenic capacity was determined through alkaline phosphatase (ALP) and alizarin red staining (ARS). Our findings indicated that macroporous scaffolds enhanced macrophage adhesion and drove their differentiation towards the M2 phenotype. This led to the increased production of anti-inflammatory factors and a reduction in pro-inflammatory agents, highlighting the scaffolds' immunomodulatory capabilities. Moreover, conditioned media from macrophages cultured on these macroporous scaffolds bolstered the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), exhibiting superior osteogenic differentiation potential. Consequently, FDM-fabricated PCL scaffolds, with precision-controlled pore sizes, present promising prospects as superior materials for bone tissue engineering, leveraging the regulation of macrophage polarization.

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Section: Preparation Of Scaffoldssupporting

confidence: 86%

Section: Cell Proliferation Assaymentioning

confidence: 99%

3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration

Wang,

Fu,

Luo

et al. 2024

Biomed. Mater.

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“…Other studies include the following: An electrospun nanofiber from synthetic poly (ethylene terephthalate) (PET) mats with an amine-rich thin plasma-polymerised coating was studied to improve HUVEC cell adhesion, reduce thrombosis and provide stable endothelial lining for vascular grafts [ 45 ]. Amine plasma polymerisation of a 3D bioscaffold from polycaprolactone and beta-tricalcium phosphate was also investigated to improve osteogenic differentiation [ 46 ]. A nanofiber from bacterial-sourced polyhydroxy butyrate (PHB) was modified by plasma polymerisation using polyethylene glycol (PEG) and ethylenediamine (EDA), and indicated differences in defending oxidative stress and cell attachment [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Plasma-Polymerised Antibacterial Coating of Ovine Tendon Collagen Type I (OTC) Crosslinked with Genipin (GNP) and Dehydrothermal-Crosslinked (DHT) as a Cutaneous Substitute for Wound Healing

et al. 2023

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Tissue engineering products have grown in popularity as a therapeutic approach for chronic wounds and burns. However, some drawbacks include additional steps and a lack of antibacterial capacities, both of which need to be addressed to treat wounds effectively. This study aimed to develop an acellular, ready-to-use ovine tendon collagen type I (OTC-I) bioscaffold with an antibacterial coating for the immediate treatment of skin wounds and to prevent infection post-implantation. Two types of crosslinkers, 0.1% genipin (GNP) and dehydrothermal treatment (DHT), were explored to optimise the material strength and biodegradability compared with a non-crosslinked (OTC) control. Carvone plasma polymerisation (ppCar) was conducted to deposit an antibacterial protective coating. Various parameters were performed to investigate the physicochemical properties, mechanical properties, microstructures, biodegradability, thermal stability, surface wettability, antibacterial activity and biocompatibility of the scaffolds on human skin cells between the different crosslinkers, with and without plasma polymerisation. GNP is a better crosslinker than DHT because it demonstrated better physicochemical properties (27.33 ± 5.69% vs. 43 ± 7.64% shrinkage), mechanical properties (0.15 ± 0.15 MPa vs. 0.07 ± 0.08 MPa), swelling (2453 ± 419.2% vs. 1535 ± 392.9%), biodegradation (0.06 ± 0.06 mg/h vs. 0.15 ± 0.16 mg/h), microstructure and biocompatibility. Similarly, its ppCar counterpart, GNPppCar, presents promising results as a biomaterial with enhanced antibacterial properties. Plasma-polymerised carvone on a crosslinked collagen scaffold could also support human skin cell proliferation and viability while preventing infection. Thus, GNPppCar has potential for the rapid treatment of healing wounds.

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“…The contact angle of PCL-HAp decreased significantly from 71.59 + 2.20° to 63.55 + 2.22°, 65.39 + 2.33°, and 57.15 + 1.87° by reinforcement with collagen, gelatin, and chitosan, respectively. Generally, the surface wettability of the scaffold is increased by the introduction of the polar functional groups to the surfaces of polymers (Kim et al, 2022). The HAp-PCL-Ch composite showed maximum wettability amongst all samples, suggesting the high contribution of positively charged deacetylated amine groups to composite wettability.…”

Section: O N L I N E F I R S Tmentioning

confidence: 98%

A facile technique for overcoming seeding barriers of hydrophobic polycaprolactone/hydroxyapatite-based nanofibers for bone tissue engineering

Nareswari¹,

Juniatik²,

Aminatun³

et al. 2022

J App Pharm Sc

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Matrix hydrophobicity hinders cell attachment in tissue engineered scaffolds. We proposed a facile seeding method to optimize cell presentation in nanofibrous mats formulated in a mixture of hydrophobic polycaprolactone (PCL), hydroxyapatite (HAp) mimicking bone surface roughness, and a ligand-supporting biopolymer. High-density osteoblast suspensions in serum-deficient media were seeded into composites arranged on a glass carrier sandwiched between cylindrical supports (seeding constructs) for 4 hours initial seeding and subsequently statically cultured in a complete medium for 7 days. Cell behavior and growth were analyzed by viability assays, LIVE/DEAD fluorescence labeled imaging, and electron microscopy. The zeta potential and contact angle of PCL-HAp nanofibers were altered by the addition of biopolymers, which directed cell attachment and proliferation. Modified seeding proved the benefit of collagen reinforcement to mediate cell-matrix interactions, which was demonstrated by enhancing cell spread with nearly twofold substantial growth during culture. The addition of gelatin showed a lower level of increased cell adhesion than collagen. Interestingly, clusters of spheroid cells were found in the chitosan composite with high cell adhesion on the first day, but the cells did not grow further until the end of the culture. In contrast, poor cell adhesion and inconsistent growth were found after conventional seeding and thus emphasized the potential role of modified seeding in supporting matrix performance as a cell carrier.

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Amine Plasma-Polymerization of 3D Polycaprolactone/β-Tricalcium Phosphate Scaffold to Improving Osteogenic Differentiation In Vitro

Cited by 15 publications

References 67 publications

3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration

3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration

Plasma-Polymerised Antibacterial Coating of Ovine Tendon Collagen Type I (OTC) Crosslinked with Genipin (GNP) and Dehydrothermal-Crosslinked (DHT) as a Cutaneous Substitute for Wound Healing

A facile technique for overcoming seeding barriers of hydrophobic polycaprolactone/hydroxyapatite-based nanofibers for bone tissue engineering

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