Aminoglycosides–Loaded Glucose-Conjugated Chitosan Nanoparticles for In vitro Antimicrobial and Antibiofilm Screening on Klebsiella pneumonia

Pourebrahim, Moslem; Nejabatdoust, Armin; Mirmiran, Seyyed Danial; Daemi, Hakimeh Baghaei; Meftahpour, Haniye; Salehzadeh, Ali

doi:10.1007/s12668-021-00878-7

Cited by 6 publications

(8 citation statements)

References 30 publications

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“…Consequently, the expression of biofilm genes is diminished. Based on the findings, it can be inferred that thymol-CsNG has the potential to be a good candidate for improving antibacterial and anti-biofilm properties ( Pourebrahim et al, 2021 ; Piri-Gharaghie et al, 2022 ).…”

Section: Thymolmentioning

confidence: 98%

“…In this regard, Piri-Gharaghie et al also fabricated thymol-loaded chitosan nanogels. These nanogels can be used as a drug delivery system due to their low cytotoxicity, biodegradability, and biocompatibility ( Pourebrahim et al, 2021 ). The antibacterial activity test showed that the thymol-loaded chitosan nanogel (thymol -CsNG) decreased the MIC by 4–6 times compared to free thymol.…”

Section: Thymolmentioning

confidence: 99%

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Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Shariati,

Noei,

Askarinia

et al. 2024

Front. Pharmacol.

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Pseudomonas aeruginosa biofilm is a community of bacteria that adhere to live or non-living surfaces and are encapsulated by an extracellular polymeric substance. Unlike individual planktonic cells, biofilms possess a notable inherent resistance to sanitizers and antibiotics. Overcoming this resistance is a substantial barrier in the medical and food industries. Hence, while antibiotics are ineffective in eradicating P. aeruginosa biofilm, scientists have explored alternate strategies, including the utilization of natural compounds as a novel treatment option. To this end, curcumin, carvacrol, thymol, eugenol, cinnamaldehyde, coumarin, catechin, terpinene-4-ol, linalool, pinene, linoleic acid, saponin, and geraniol are the major natural compounds extensively utilized for the management of the P. aeruginosa biofilm community. Noteworthy, the exact interaction of natural compounds and the biofilm of this bacterium is not elucidated yet; however, the interference with the quorum sensing system and the inhibition of autoinducer production in P. aeruginosa are the main possible mechanisms. Noteworthy, the use of different drug platforms can overcome some drawbacks of natural compounds, such as insolubility in water, limited oral bioavailability, fast metabolism, and degradation. Additionally, drug platforms can deliver different antibiofilm agents simultaneously, which enhances the antibiofilm potential of natural compounds. This article explores many facets of utilizing natural compounds to inhibit and eradicate P. aeruginosa biofilms. It also examines the techniques and protocols employed to enhance the effectiveness of these compounds.

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Section: Thymolmentioning

confidence: 98%

Section: Thymolmentioning

confidence: 99%

Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Shariati,

Noei,

Askarinia

et al. 2024

Front. Pharmacol.

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“…Therefore, given the widespread drug resistance and therapeutic failure that is being reported daily around the world to commonly used antibiotics, the need to nd an alternative way to maximize the effectiveness of antibiotics is more needed than ever to review (https://www.who.int/publications/i/item/9789240021303). Chitosan (Cs) contain β-1,4-linked 2-amino-2-deoxy-β-D-glucose (deacetylated D-glucosamine) and N-acetyl-D-glucosamine units are biocompatible, biodegradable, and non-toxic compounds which are derived from chitin, so that it can be used in numerous medical applications as antitumor, immunoenhancing, antifungal, antimicrobial, antioxidant and wound healing biomaterials (Shariatinia, 2019;Pourebrahim et al, 2021). Structurally, chitosan nanoparticles are obtained using chitosan and sodium tripolyphosphate (STPP) by the ionic gelation method.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the interaction of chitosan with the bacterial cell, it has been suggested that positive charges on the surface of chitosan make it possible to bind to the negative part of the bacterial cell membrane (Pourebrahim et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effective Release of Ciprofloxacin and Rifampicin Antibiotics from Alginate-Chitosan Complex and Its Application Against Clinical Strains of Staphylococcus aureus

Nejabatdoust,

Mirmiran,

Salehzadeh

et al. 2023

BioNanoSci.

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This study aimed to characterize the antimicrobial and anti-bio lm activity of cipro oxacin (CIP) and rifampicin (RMP) loaded on the calcium alginate (CaAlg) backbone through the electrostatic interaction of CaAlg/antibiotic (s) and chitosan-TPP nanoparticles (Cs-TPP NPs). The shape and functional groups were identi ed by scanning electron microscope (SEM), Fourier transform infrared (FT-IR) spectroscopy. SEM images show that the nanoparticles are spherical and granular with an average size of about 100-500 nm. FT-IR results showed bonds corresponding to the Cs-TPP NPs and CaAlg-Antibiotics (CaAlg-CIP, CaAlg-RMP) after the loading process. The tube adherence method (TM) was used to screen potent bio lm producers. The antibio lm capacity of Cs/CaAlg-CIP and Cs/CaAlg-RMP, along with free antibiotics was used against clinical strains in the tube method and results showed that the use of sub-MICs of nano-drugs ( 1 / 2 MIC) inhibited bio lm formation in the tested strains compared to free CIP and RMP (P < 0.05). The potential antimicrobial effect of Cs/CaAlg-CIP and Cs/CaAlg-RMP NPs against clinical strains was also evaluated using minimum inhibitory concentration (MICs) and a time-depending killing method. A robust inhibitory effect on bacterial growth even at low concentrations of Cs/CaAlg-CIP and Cs/CaAlg-RMP NPs (CONC 4 and 2 µg.mL ─1 ) was observed compared with free drugs (CIP at 32 and RMP at 8.0 µg.mL ─1 ), (P < 0.05). The time-kill kinetics assay showed that Cs/CaAlg-CIP and Cs/CaAlg-RMP NPs had faster and more lasting antibacterial effects than the free drug (s) on the tested strains. This study shows that loading CIP and RMP into CaAlg scaffolds and complexes with chitosan-TPP nanoparticles using the emulsi cation/internal gelation method increases the antimicrobial activity of antibiotics against clinical strains of S. aureus and thus might serve as a new type of antimicrobial compounds for the treatment of bacterial infections.

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“…[15] Chitosan nanogels can be used as a drug delivery system due to their low cytotoxicity, biodegradability and biocompatibility. [16] Chitosan is a degradable nanocarrier with the ability to bind to microbial cells, which makes it an antimicrobial nanocarrier. [17] In this study, thymol was encapsulated in chitosan nanogel as a novel pharmaceutical formulation studied in terms of encapsulation efficiency, in vitro drug release pattern, and stability properties.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Thymol‐Loaded Chitosan Nanogels: Improved Antibacterial and Anti‐Biofilm Activities with Negligible Cytotoxicity

Gharaghie

Beiranvand

Riahi

et al. 2022

Chemistry & Biodiversity

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Thymol is a monoterpene phenolic derivative extracted from the Thymus vulgaris which has antimicrobial effects. In the present study, thymol-loaded chitosan nanogels were prepared and their physicochemical properties were characterized. The encapsulation efficiency of thymol into chitosan and its stability were determined. The in vitro antimicrobial and anti-biofilm activities of thymol-loaded chitosan nanogel (Ty-CsNG), free thymol (Ty), and free chitosan nanogel (CsNG) were evaluated against both Gram-negative and Grampositive multidrug-resistant (MDR) bacteria including Staphylococcus aureus, Acinetobacter baumanii, and Pseudomonas aeruginosa strains using the broth microdilution and crystal violet assay, respectively. After treatment of MDR strains with sub-minimum inhibitory concentration (Sub-MIC) of Ty-CsNG, free Ty and CsNG, biofilm gene expression analysis was studied. Moreover, cytotoxicity of Ty-CsNG, free Ty, and CsNG against HEK-293 normal cell line was determined using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The average size of Ty-CsNG was 82.71 � 9.6 nm, encapsulation efficiency was 76.54 � 0.62 % with stability up to 60 days at 4 °C. Antibacterial activity test revealed that Ty-CsNG reduced the MIC by 4 -6 times in comparison to free thymol. In addition, the expression of biofilm-related genes including ompA, and pgaB were significantly down-regulated after treatment of strains with Ty-CsNG (P < 0.05). In addition, free CsNG displayed negligible cytotoxicity against HEK-293 normal cell lines and presented a biocompatible nanoscale delivery system. Based on the results, it can be concluded that Ty-CsNG can be considered a promising candidate for enhancing antimicrobial and anti-biofilm activities.

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Aminoglycosides–Loaded Glucose-Conjugated Chitosan Nanoparticles for In vitro Antimicrobial and Antibiofilm Screening on Klebsiella pneumonia

Cited by 6 publications

References 30 publications

Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community

Effective Release of Ciprofloxacin and Rifampicin Antibiotics from Alginate-Chitosan Complex and Its Application Against Clinical Strains of Staphylococcus aureus

Fabrication and Characterization of Thymol‐Loaded Chitosan Nanogels: Improved Antibacterial and Anti‐Biofilm Activities with Negligible Cytotoxicity

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