Ammonium and phosphate limitation in 1,3-propanediol production by Klebsiella pneumoniae

Zheng, Zongming; Xu, Yun‐Zhen; Wang, Tipeng; Dong, Changqing; Yang, Yongping; Liu, Dehua

doi:10.1007/s10529-009-0150-y

Cited by 10 publications

(5 citation statements)

References 15 publications

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“…The exact mechanism involved in the effect of ammonium ion on the production of PDO is still unknown. However, the evidence for the impact of ammonia limited media on the glycerol dissimilation to produce PDO has also been provided by Zheng et al [ 16 ]. Furthermore, H. hydrogeniformans is an anaerobic bacteria and requires a reducing environment.…”

Section: Discussionmentioning

confidence: 99%

The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans

Kalia

Trager

Sitton

et al. 2016

Life

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In recent years, biodiesel, a substitute for fossil fuels, has led to the excessive production of crude glycerol. The resulting crude glycerol can possess a high concentration of salts and an alkaline pH. Moreover, current crude glycerol purification methods are expensive, rendering this former commodity a waste product. However, Halanaerobium hydrogeniformans, a haloalkaliphilic bacterium, possesses the metabolic capability to convert glycerol into 1,3-propanediol, a valuable commodity compound, without the need for salt dilution or adjusting pH when grown on this waste. Experiments were performed with different combinations of 24 medium components to determine their impact on the production of 1,3-propanediol by using a fractional factorial design. Tested medium components were selected based on data from the organism’s genome. Analysis of HPLC data revealed enhanced production of 1,3-propanediol with additional glycerol, pH, vitamin B12, ammonium ions, sodium sulfide, cysteine, iron, and cobalt. However, other selected components; nitrate ions, phosphate ions, sulfate ions, sodium:potassium ratio, chloride, calcium, magnesium, silicon, manganese, zinc, borate, nickel, molybdenum, tungstate, copper and aluminum, did not enhance 1,3-propanediol production. The use of a fractional factorial design enabled the quick and efficient assessment of the impact of 24 different medium components on 1,3-propanediol production from glycerol from a haloalkaliphilic bacterium.

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Section: Discussionmentioning

confidence: 99%

The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans

Kalia

Trager

Sitton

et al. 2016

Life

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“…Excessively vigorous stirring has been found to decrease 1,3-PD production, probably due to over-aeration of the medium [40], or to have a negative effect on bacterial growth [33]. Excessively low stirring rate, on the other hand, led to the accumulation of 3-HPA [16,38]. The value of 80 rpm was selected as the optimal stirring rate, as it prevents foaming and additional, unjustified power demand.…”

Section: Impact Of Stirring Rate On Products Formationmentioning

confidence: 99%

“…However, the accumulation of 3-HPA during the microbial production of 1,3-PD leads to negative consequences which include cell growth limitation and premature cessation of the process. There are numerous reports on this phenomenon [1,3,[16][17][18][19]. It is known that 3-HPA induces oxidative stress in the cells, and its dimer inhibits the synthesis of DNA [18,20].…”

Section: Introductionmentioning

confidence: 98%

The Impact of Process Parameters on 1,3-Propanediol Production and 3-Hydroxypropionaldehyde Accumulation in Fed-Batch Fermentation of Glycerol with Citrobacter freundii AD119

et al. 2023

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Microbial production of 1,3-propanediol (1,3-PD) has attracted the interest of scientists for decades. Its product offers an environmentally friendly and sustainable alternative to fossil-based raw materials for chemical synthesis. Citrobacter freundii is one of the natural producers of 1,3-PD known for its ability to yield it in significant titers. An efficient bioprocess requires an in-depth understanding of the factors that influence the performance of its biocatalyst. The effects of pH, temperature, stirring rate, and substrate concentration on glycerol fermentation in fed-batch cultures of C. freundii AD119 were investigated in this study. In addition to monitoring the kinetics of substrate utilization and the formation of the final products, the concentration of 3-hydroxypropionaldehyde (3-HPA), an inhibitory intermediate of glycerol bioconversion, was analyzed. When the optimal working conditions were used (pH 7.0, temperature 30 °C, stirring rate of 80 rpm, and glycerol concentration below 15 g/L during the fed-batch phase), 53.44 g/L of 1,3-PD were obtained. When the process was performed at temperatures of 33 °C or higher or in acidic pH (6.5), an elevated concentration of 3-HPA was observed and the process halted prematurely.

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“…The most significant factors, affecting the fermentation are the medium content, the aeration regime, and pH. The first two influencing factors were a subject of detailed research by a number of scientific experiments [4][5][6][7] and were optimized to a great extend. In contrast, pH requirements for predominant 1,3-PD production and low by-products receiving at the same time, still to be obscure until now [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations

Petrov

Stoyanov

2011

Bioprocess Biosyst Eng

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1,3-Propanediol (1,3-PD) is a bivalent alcohol, used in a number of chemical syntheses. It could be produced from glycerol in course of microbial fermentation by Klebsiella pneumoniae along with more than five minor liquid products. With the purpose to enhance 1,3-PD production and to eliminate by-products formation, principally new pH control on the process was applied. The method, named "forced pH fluctuations" was realized by consecutive raisings of pH with definite ΔpH amplitude (ranging from 1.0 to 2.0) at time intervals between 2 and 4 h, during a series of fed batch processes. The fermentation performed by forced pH fluctuations with ΔpH = 1.0, risen at every 3 h was evaluated as the most successful. Increase by 10% of the maximal amount of 1,3-PD (g/l), 22% higher productivity [g/(l h)], and 29% increase in 1,3-PD molar yield were achieved, compared to the referent fed batch (with constant pH = 7.0). In addition, significant decrease in by-products formation was obtained. The most important reduction was observed in the lactic and acetic acids yields, where 50 and 70% decrease were reached. The results suggested the potential of pH to manage the share and quantity of product spectrum in mixed diols-acids fermentations. The application of "forced pH fluctuations method" achieves the desirable increase in 1,3-PD formation and decrease in by-products accumulation at the same time by a comparatively simple approach by adjustment of one bioprocess parameter only.

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Ammonium and phosphate limitation in 1,3-propanediol production by Klebsiella pneumoniae

Cited by 10 publications

References 15 publications

The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans

The Use of a Fractional Factorial Design to Determine the Factors That Impact 1,3-Propanediol Production from Glycerol by Halanaerobium hydrogeniformans

The Impact of Process Parameters on 1,3-Propanediol Production and 3-Hydroxypropionaldehyde Accumulation in Fed-Batch Fermentation of Glycerol with Citrobacter freundii AD119

Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations

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