Amnion membrane hydrogel and amnion membrane powder accelerate wound healing in a full thickness porcine skin wound model

Abstract

There is a need for effective wound treatments that retain the bioactivity of a cellular treatment, but without the high costs and complexities associated with manufacturing, storing, and applying living biological products. Previously, we developed an amnion membrane-derived hydrogel and evaluated its wound healing properties using a mouse wound model. In this study, we used a full thickness porcine skin wound model to evaluate the wound-healing efficacy of the amnion hydrogel and a less-processed amnion product comprising a lyophilized amnion membrane powder. These products were compared with commercially available amnion and nonamnion wound healing products. We found that the amnion hydrogel and amnion powder treatments demonstrated significant and rapid wound healing, driven primarily by new epithelialization versus closure by contraction. Histological analysis demonstrated that these treatments promote the formation of a mature epidermis and dermis with similar composition to healthy skin. The positive skin regenerative outcomes using amnion hydrogel and amnion powder treatments in a large animal model further demonstrate their potential translational value for human wound treatments.

Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions

“…Regenerative solutions for complex CMF defects will likely require the use of biomaterials that promote osteogenesis and modulate the inflammatory response to accelerate healing. Herein, we address the challenge of inflammation by utilizing the amniotic membrane, which has been known to be anti-inflammatory (27)(28)(29)(30)(31). Modulating the inflammatory response of implants can increase the likelihood of success, by preventing fibrous encapsulation of implants which can occur due to persistent inflammation (5).…”

“…The addition of mineral to collagen scaffolds has been shown to promote osteogenesis and mineral formation in vitro and in vivo, even in the absence of osteogenic media and BMP-2 supplements to enhance bone formation (15,26). Recently, the amniotic membrane derived from placentas has been investigated as a natural extracellular matrix that could be used in biomaterial implants for its anti-inflammatory properties (27)(28)(29)(30)(31). In addition, amniotic membrane extracts have been shown to provide growth factors for osteogenic differentiation and repair bone defects (32)(33)(34)(35)(36).…”

A Novel Nanofiber Hydrogel Loaded with Platelet-Rich Plasma Promotes Wound Healing Through Enhancing the Survival of Fibroblasts

“…SAPNS represents an advancement in biological materials, due to its ability to absorb moisture and insoluble 3-dimensional polymer hydrogels. Currently, sNAG hydrogels have been used in clinical practice as biological scaffolds [20,21]. Recent studies have reported that hydrogels can be encapsulated mesoporous silicon coated gold nanoshells as the only drug transporter [22], achieving outstanding clinical efficacy.…”

Recombinant Treponema pallidum protein Tp0136 promotes fibroblast migration by modulating MCP‐1/CCR2 through TLR4

“…Wound healing involves a variety of mechanisms . Among them, one of the most important mechanisms is the migration of fibroblasts to fill the injured lesion .…”

“…Wound healing involves a variety of mechanisms. 4,5 Among them, one of the most important mechanisms is the migration of fibroblasts to fill the injured lesion. 6 The migration of fibroblasts is regulated by a variety of cytokines, including monocyte chemotactic protein-1 (MCP-1), interleukin (IL)-6 and matrix metalloproteinase (MMP)-9.…”