<i> Annonaa muricata </i> Linn Leaf Induce Apoptosis in Cancer Cause Virus

Abstract

Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in humans was included the loss of p53 protein function due to mutations in the protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24 well plate micro-cultures. HeLa cells are as 2 × 10 4 cells in 100 mL in RPMI media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed by the method of calculation tryphan blue dye exclusion. Being fasted for 24 hours in the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these wells is calculated the number of living cells and made the curve of cell number and incubation time. Result: The results showed that HeLa cells are being LC50 partition of leaves Annona muricata Linn in ethyl acetate his cell death rate was higher (2000 µg/ml have 131.89%; 15.625 µg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower (2000 µg/ml have 35.80%; 15.625 µg/ml have 3.97%). Another results showed that HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher (2000 µg/ml have 91.86%; 15.625 µg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 µg/ml have 23.79%; 15.625 µg/ml have 4.69%). Figure regression LC50 of HeLa cell culture treatment with partition or fractionation looks of regression test is the positive regression coefficient. Conclusion: Annona muricata Linn leaf in chloroform is a good candidate for chemoprevention escort chemotherapy for cancer causing viruses.

The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line

“…In the study conducted by Fidaningsih and Handayani, it is found that the best effect of the water extract of soursop leaves to inhibit proliferation is in the dosage of 75 mg/ml with an 88.45% inhibition rate [24]. On the other hand, in another study by Astirin et al, it is found that the dosage needed for alcohol-soluble soursop leaf extract to achieve the same effect is 2 mg/ml with 35.80% apoptotic figure [25]. Another study which assessed the effect of the leaves of Annona muricata against cancer cells described that the leaf extract could induce apoptosis in colon and lung cancer through the mitochondria and has the effect of activation of caspase-3 in leukemia.…”

Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells

“…A. muricata leaves were successively extracted using soxhlet apparatus [19][20][21][22]. A total of 55 g of A. muricata powdered leaves were first extracted with n-hexane until the solution turned colourless.…”

Jatropha Curcas Linn can Reduce the Expression of Hsp70 that will Result in Reduced Errors in Protein Folding and Promotion of Normal Protein Function in Proliferation and Apoptosis

“…If will be used then dissolved in a little phosphate of sodium 5 mM, pH 6.5 without NaCl. A solution of vortexed until late at, centrifuged, the sediment dumped and supernatant which is the fraction of protein included in conical tube [19,20]. Partition done with chloroform and the success of a partition monitored with the methods chromatography thin layer and a top part is taken fractionation of from the upper part of the results of the process of partition is by using liquid chromatography vacuum.…”

“…Subsequently a precipitate during 24hr in culture medium than the Raji cell were grown in microplate with media plus extract with a nonlethal concentration of fractionation J. curcas leaf. of sampling was performed for 24 hrs [20].…”

“…All fractions produced monitored the profile of its chemical content using chromatography thin layer method until fractions have a profile almost the same merged into one faction. CFaction chosen (combined F12 and F13) is who assigns grades the percentage of death cell line that was the greatest [19,20].…”