<i>In Vitro</i> Antioxidant Properties of Phloretin—An Important Phytocompound

Abstract

Reactive oxygen species [ROS] cause oxidative damage to the tissues and protection from such damages is provided by endogenous and exogenous antioxidants. Much research work has been carried out in recent years on the beneficial effect of phenolic compounds which act as natural antioxidants and help to neutralize free radicals. So, this study was aimed to evaluate the in vitro antioxidant capacity of one of the phenolic compounds phloretin. Phloretin was used at four different concentrations like 20, 40, 60 and 80 µg/ml to determine the antioxidant activity by different methods such as total antioxidant capacity, reducing power, DPPH radical scavenging, superoxide anion radical scavenging and metal chelating assays. In addition to that the ascorbic acid was used as reference compound. The results showed that the phloretin displayed potent in vitro antioxidant capacity. It was able to scavenge different in vitro free radicals in all tested concentrations. Among the different concentrations, 80 µg of phloretin has maximum activity when compared to other concentrations in all in vitro antioxidant assays. High antioxidant property and maximum protective effect of phloretin were observed in a concentration dependent manner. The results were expressed as IC50 value. The lowest IC50 value indicates the highest scavenging activity. The reducing power of the phloretin was also found in concentration dependent. According to the results of this study, we concluded that the phloretin possesses antioxidant property. Therefore, phloretin is a powerful antioxidant phytocompound which can protect biological systems against the oxidative stress. From this study, we suggest that the phloretin may be used as a dietary natural antioxidant supplement for preventing free radical related diseases.

Gc-MS Profiling, Chemical Characterization, Antioxidant, Α-Amylase and Α-Glucosidase Inhibition of Selected Seaweeds From Southeast Coast of India: An in Vitro Approach

“…Presently, the GC-MS analysis of SWEA revealed the presence of 8 active compounds viz; Phloretin, Heptadecanoic acid, Oleic acid, 5-p-coumaroylquinic acid, Phloretinrhamnosyl(1→6) glucoside, Quercetin-3-Oarabinpyranoside, Caffeic acid hexoside and Squalene. Among them, Phloretin was found to have antioxidant activity as reported earlier 46 . The previous research on GC-MS analysis of Grateloupia elliptica revealed the presence of squalene that has been reported to have antioxidant, anticancer and anti-infectant effects.…”

Lactoperoxidase inhibition of some natural phenolic compounds: Kinetics and molecular docking studies

“…Hydrogen bond is represented with black dashed line, π interaction is represented with green dashed line, and salt bridge is represented with pink dashed line had great interest with its beneficial effects. It is a dihydrochalcone flavonoid that shows a large spectrum of biological activities including antioxidant, antitumor, antibiosis, and antidiabetic effects (Nithiya & Udayakumar, 2016).…”

“…It is known that there are more than 8,000 polyphenols, up to now. It is a dihydrochalcone flavonoid that shows a large spectrum of biological activities including antioxidant, antitumor, antibiosis, and antidiabetic effects (Nithiya & Udayakumar, 2016). Phenolic acids are defined by having a benzene ring, a carboxyl group and one or more hydroxyl and/or methoxy groups in the molecule (Bravo, 1998).…”

Effect of phloretin on growth performance, serum biochemical parameters and antioxidant profile in heat-stressed broilers

“…Consistent with these results, the present study showed that heat stress significantly reduced the expression of serum antioxidant factors in broilers, while phloretin can increase the concentration of these factors. The ability of phloretin to relieve the body's oxidative stress is related to its effect on inducing changes in the redox state of cells ( Nithiya and Udayakumar 2016 ; Han et al., 2020 ). Huang et al.…”