2006
DOI: 10.1016/j.msec.2005.10.031
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Amperometric biosensor for ethanol detection based on alcohol oxidase immobilised within electrochemically deposited Resydrol film

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Cited by 64 publications
(27 citation statements)
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“…Electrochemical deposition of the polymer film has been achieved by applying the potentiostatic pulse profile consisting of 20 consecutive pulses at +1900 mV for 0.3 s and at −300 mV for 5 s. The minimal detection limit was 3.5.10 −2 (%, v/v) of ethanol. The biosensor developed has showed its potential for ethanol detection in alcoholic beverages (Shkotova et al 2006).…”
Section: Ethanolmentioning
confidence: 99%
“…Electrochemical deposition of the polymer film has been achieved by applying the potentiostatic pulse profile consisting of 20 consecutive pulses at +1900 mV for 0.3 s and at −300 mV for 5 s. The minimal detection limit was 3.5.10 −2 (%, v/v) of ethanol. The biosensor developed has showed its potential for ethanol detection in alcoholic beverages (Shkotova et al 2006).…”
Section: Ethanolmentioning
confidence: 99%
“…It is a new technology of proteins immobilization based on electrochemical precipitation into the polyanionic polymer matrix owing to its solubility change during pH-modulation. Commercial resin Resydrol is a mixture of acrylic copolymers, protonisation and solubility of which depend on pH value [22,23].…”
Section: Go Immobilization By Electrochemical Deposition In the Polymmentioning
confidence: 99%
“…Primary tension (+1900 mV during 1 s) initiated water oxidation. Further tension impulses (−300 mV during 5 s) caused transient pH-modulation and subsequent binding of enzyme molecules in the Resydrol polyanionic matrix [23].…”
Section: Go Immobilization By Electrochemical Deposition In the Polymmentioning
confidence: 99%
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“…Yeast alcohol oxidase (AOX; alcohol:O 2 oxidoreductase, EC 1.1.3.13) has been extensively used for the determination of lower primary alcohols and formaldehyde (Alpeeva et al, 2005;Shkotova et. al., 2006).…”
Section: Development An Amperometric Biosensor Based On Mutated Alcohmentioning
confidence: 99%