Amperometric Determination of Quercetin in Some Foods by Magnetic Core/Shell Fe3O4@ZnO Nanoparticles Modified Glassy Carbon Electrode

Arvand, Majid; Chaibakhsh, Naz; Daneshvar, Samaneh

doi:10.1007/s12161-014-0080-8

Cited by 17 publications

(3 citation statements)

References 53 publications

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“…For calculating the LOD of PCR‐coupled CRISPR‐BLI, we first obtained a linear calibration curve by performing a logarithmic operation to the input DNA amount and output signal (log[DNA] vs log[BLI signal]). The linear range of PCR‐coupled CRISPR‐BLI was 20 to 20 000 pg ( R 2 =0.9963; Figure 6e), and the LOD was 1.34 pg according to the equation: [45]

true S / N = 3

…”

Section: Resultsmentioning

confidence: 99%

Construction of a CRISPR‐Biolayer Interferometry Platform for Real‐Time, Sensitive, and Specific DNA Detection

Qiao

Liu

et al. 2021

ChemBioChem

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The clustered regularly interspaced short palindromic repeats (CRISPR) technology has been widely applied for nucleic acid detection because of its high specificity. By using the highly specific and irreversible bond between HaloTag and its alkane chlorine ligand, we modified dCas9 (deactivated CRISPR/Cas9) with biotin as a biosensor to detect nucleic acids. The CRISPR biosensor was facilely prepared to adequately maintain its DNA‐recognition capability. Furthermore, by coupling biolayer interferometry (BLI) with the CRISPR biosensor, a real‐time, sensitive, and rapid digital system called CRISPR‐BLI was established for the detection of double‐stranded DNA. The CRISPR biosensor immobilised on the biolayer could recruit the target DNA onto the biosensor surface and change its optical thickness, resulting in a shift in the interference pattern and responding signal of the BLI. The CRISPR‐BLI system was further applied to detect the ALP gene of Escherichia coli DH5α combined with a polymerase chain reaction, which demonstrated a linear range from 20 to 20 000 pg and a low detection limit (1.34 pg). The CRISPR‐BLI system is a promising approach for rapid and sensitive detection of target DNA analytes.

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true S / N = 3

…”

Section: Resultsmentioning

confidence: 99%

Construction of a CRISPR‐Biolayer Interferometry Platform for Real‐Time, Sensitive, and Specific DNA Detection

Qiao

Liu

et al. 2021

ChemBioChem

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“…Previous studies have shown that electrochemical detection is very suitable for the detection of Qu [17,18]. For example, Arvand et al [19] successfully prepared an amperometric determination Micromachines 2021, 12, 1519 2 of 10 sensor and used magnetic Core/Shell Fe 3 O 4 @ZnO nanoparticles modified on GCE to detect Qu by means of cyclic voltammetry (CV), with a detection limit of 0.16 µmol•L −1 . Ma et al [20] used three-dimensional reduced graphene oxide aerogel modified on electrodes to construct a sensor to realize the detection of Qu by means of differential pulse voltammetry (DPV), with a detection limit of 0.065 µmol•L −1 .…”

Section: Introductionmentioning

confidence: 99%

Convenient Heme Nanorod Modified Electrode for Quercetin Sensing by Two Common Electrochemical Methods

et al. 2021

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Quercetin (Qu) is one of the most abundant flavonoids in the human diet. High concentrations of Qu can easily cause adverse effects and induce inflammation, joint pain and stiffness. In this study, Heme was used as a sensitive element and deposited and formed nanorods on a glassy carbon electrode (GCE) for the detection of Qu. The Heme/GCE sensor was characterized using scanning electron microscopy (SEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimized conditions, the developed sensor presented a linear concentration ranging from 0.1 to 700 μmol·L−1 according to the CV and DPV methods. The detection limit for the sensor was 0.134 μmol·L−1 and its sensitivity was 0.12 μA·μM−1·cm−2, which were obtained from CV analysis. Through DPV analysis we obtained a detection limit of 0.063 μmol·L−1 and a sensitivity of 0.09 μA·μM−1·cm−2. Finally, this sensor was used to detect the Qu concentration in loquat leaf powder extract, with recovery between 98.55–102.89% and total R.S.D. lower than 3.70%. The constructed electrochemical sensor showed good anti-interference, repeatability and stability, indicating that it is also usable for the rapid detection of Qu in actual samples.

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“…[1][2][3][4] Flavonols belong to the family of flavonoids and are mainly divided into quercetin, kaempferol and myricetin. 5 Quercetin (QR, 3,3′,4′,5,7pentahydroxylflavone) is the most daily consumption dietary flavonol. As a result of its biological activities, for example inhibition of cell degradation and damage, treatment of endocrine diseases and chronic bronchitis, protection of cardiovascular, prevention of cataract, regulation of the immune system and so on, quercetin is often added as the main ingredient in many kinds of drugs.…”

mentioning

confidence: 99%

An Excellent Electrochemical Sensor Based on Highly Porous Gold Film Modified Gold Electrode for Detecting Quercetin in Food and Medicine

Liu

Xie

Yang

et al. 2020

J. Electrochem. Soc.

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Quercetin is one of the most important nutrients in human daily diet, the accurate and efficient analytical approaches for quercetin is highly required as it's closely related to many biological activities. In this paper, the three-dimensional highly porous gold film (hp-Au) modified gold electrode (Au) was prepared by a facile two steps self-templating method, it was found that hp-Au possess high surface-to-volume ratio and excellent conductivity. Combining with the multiple merits of hp-Au, the hp-Au/Au showed an excellent detection performance towards quercetin compared with bare electrode and Au nanoparticles modified gold electrode (AuNPs/Au), the detection limit was 3.9 nM in a wide concentration range from 20 nM to 100 μM. Meantime hp-Au/Au also showed decent stability, satisfied selectivity and desirable reproducibility. Moreover, the practical application of this sensor was verified by detecting QR in drink or food and medicine samples and the satisfactory recoveries of 97.43% to 101.59% and low RSD value (n = 3) no more than 3% were obtained.

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Amperometric Determination of Quercetin in Some Foods by Magnetic Core/Shell Fe3O4@ZnO Nanoparticles Modified Glassy Carbon Electrode

Cited by 17 publications

References 53 publications

Construction of a CRISPR‐Biolayer Interferometry Platform for Real‐Time, Sensitive, and Specific DNA Detection

Construction of a CRISPR‐Biolayer Interferometry Platform for Real‐Time, Sensitive, and Specific DNA Detection

Convenient Heme Nanorod Modified Electrode for Quercetin Sensing by Two Common Electrochemical Methods

An Excellent Electrochemical Sensor Based on Highly Porous Gold Film Modified Gold Electrode for Detecting Quercetin in Food and Medicine

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